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Deep tissue optical imaging of upconverting nanoparticles enabled by exploiting higher intrinsic quantum yield through use of millisecond single pulse excitation with high peak power.

Liu, Haichun LU ; Xu, Can LU ; Dumlupinar, Gökhan; Jensen, Ole B; Andersen, Peter E and Andersson-Engels, Stefan LU (2013) In Nanoscale 5(20). p.10034-10040
Abstract
We have accomplished deep tissue optical imaging of upconverting nanoparticles at 800 nm, using millisecond single pulse excitation with high peak power. This is achieved by carefully choosing the pulse parameters, derived from time-resolved rate-equation analysis, which result in higher intrinsic quantum yield that is utilized by upconverting nanoparticles for generating this near infrared upconversion emission. The pulsed excitation approach thus promises previously unreachable imaging depths and shorter data acquisition times compared with continuous wave excitation, while simultaneously keeping the possible thermal side-effects of the excitation light moderate. These key results facilitate means to break through the general shallow... (More)
We have accomplished deep tissue optical imaging of upconverting nanoparticles at 800 nm, using millisecond single pulse excitation with high peak power. This is achieved by carefully choosing the pulse parameters, derived from time-resolved rate-equation analysis, which result in higher intrinsic quantum yield that is utilized by upconverting nanoparticles for generating this near infrared upconversion emission. The pulsed excitation approach thus promises previously unreachable imaging depths and shorter data acquisition times compared with continuous wave excitation, while simultaneously keeping the possible thermal side-effects of the excitation light moderate. These key results facilitate means to break through the general shallow depth limit of upconverting-nanoparticle-based fluorescence techniques, necessary for a range of biomedical applications, including diffuse optical imaging, photodynamic therapy and remote activation of biomolecules in deep tissues. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Nanoscale
volume
5
issue
20
pages
10034 - 10040
publisher
Royal Society of Chemistry
external identifiers
  • wos:000325005500081
  • pmid:23963319
  • scopus:84884895935
ISSN
2040-3372
DOI
10.1039/c3nr01917a
language
English
LU publication?
yes
id
bdfd8bbf-2a24-435d-bcd7-4386db821c18 (old id 4005471)
date added to LUP
2013-09-10 22:10:59
date last changed
2019-02-27 01:56:29
@article{bdfd8bbf-2a24-435d-bcd7-4386db821c18,
  abstract     = {We have accomplished deep tissue optical imaging of upconverting nanoparticles at 800 nm, using millisecond single pulse excitation with high peak power. This is achieved by carefully choosing the pulse parameters, derived from time-resolved rate-equation analysis, which result in higher intrinsic quantum yield that is utilized by upconverting nanoparticles for generating this near infrared upconversion emission. The pulsed excitation approach thus promises previously unreachable imaging depths and shorter data acquisition times compared with continuous wave excitation, while simultaneously keeping the possible thermal side-effects of the excitation light moderate. These key results facilitate means to break through the general shallow depth limit of upconverting-nanoparticle-based fluorescence techniques, necessary for a range of biomedical applications, including diffuse optical imaging, photodynamic therapy and remote activation of biomolecules in deep tissues.},
  author       = {Liu, Haichun and Xu, Can and Dumlupinar, Gökhan and Jensen, Ole B and Andersen, Peter E and Andersson-Engels, Stefan},
  issn         = {2040-3372},
  language     = {eng},
  number       = {20},
  pages        = {10034--10040},
  publisher    = {Royal Society of Chemistry},
  series       = {Nanoscale},
  title        = {Deep tissue optical imaging of upconverting nanoparticles enabled by exploiting higher intrinsic quantum yield through use of millisecond single pulse excitation with high peak power.},
  url          = {http://dx.doi.org/10.1039/c3nr01917a},
  volume       = {5},
  year         = {2013},
}