Colloidal Structures in Media Simulating Intestinal Fed State Conditions with and Without Lipolysis Products
(2009) In Pharmaceutical Research 26(2). p.361-374- Abstract
- Purpose. To study the ultrastructure of biorelevant media and digestion products of self-nanoemulsifying drug delivery system (SNEDDS) at high level BS/PL conditions. Methods. Cryogenic transmission electron microscopy (Cryo-TEM) was employed to visualize the colloid structures in the biorelevant media and lipolytic products generated during hydrolysis of a SNEDDS formulation. Their electrical properties were investigated by measuring their zeta-potential values. Results. In the biorelevant media, vesicles (either unilamellar or multilamellar) and bilayer fragments are visualized. Occasionally, vesicles with an internal deformed structure are recognized, suggesting surface tension or uneven lateral stress. Visualization studies of the... (More)
- Purpose. To study the ultrastructure of biorelevant media and digestion products of self-nanoemulsifying drug delivery system (SNEDDS) at high level BS/PL conditions. Methods. Cryogenic transmission electron microscopy (Cryo-TEM) was employed to visualize the colloid structures in the biorelevant media and lipolytic products generated during hydrolysis of a SNEDDS formulation. Their electrical properties were investigated by measuring their zeta-potential values. Results. In the biorelevant media, vesicles (either unilamellar or multilamellar) and bilayer fragments are visualized. Occasionally, vesicles with an internal deformed structure are recognized, suggesting surface tension or uneven lateral stress. Visualization studies of the intermediate colloidal phases produced during digestion of a SNEDDS using the in vitro lipolysis model revealed the formation of similar structures as previously reported. The zeta-potential of the media was negatively charged and decreased from -23 to -35 mV with increasing surfactant/lipid load. Lower zeta-potential values (-16 mV) obtained for the structures formed during the lipid hydrolysis of the SNEDDS were probably due to the presence of calcium, which shields the surface, thereby reducing the charge. Conclusions. The diversity of these vesicles in terms of size, lamellarity, and internal organization advocate their important role during lipid digestion in the gastrointestinal milieu. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1312445
- author
- Fatouros, Dimitrios G. ; Walrand, Isabelle ; Bergenståhl, Björn LU and Mullertz, Anette
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- multilamellar vesicles, micelles, lipolytic products, in vitro digestion lipolysis model, microscopy, cryogenic transmission electron, zeta-potential, biorelevant media, unilamellar, systems, self-nanoemulsifying drug delivery
- in
- Pharmaceutical Research
- volume
- 26
- issue
- 2
- pages
- 361 - 374
- publisher
- Springer
- external identifiers
-
- wos:000262570400011
- scopus:58549106999
- pmid:19003522
- ISSN
- 1573-904X
- DOI
- 10.1007/s11095-008-9750-9
- language
- English
- LU publication?
- yes
- id
- be2aa4be-3787-437e-95d2-6e18dd08b63f (old id 1312445)
- date added to LUP
- 2016-04-01 12:02:00
- date last changed
- 2023-11-25 22:47:26
@article{be2aa4be-3787-437e-95d2-6e18dd08b63f,
abstract = {{Purpose. To study the ultrastructure of biorelevant media and digestion products of self-nanoemulsifying drug delivery system (SNEDDS) at high level BS/PL conditions. Methods. Cryogenic transmission electron microscopy (Cryo-TEM) was employed to visualize the colloid structures in the biorelevant media and lipolytic products generated during hydrolysis of a SNEDDS formulation. Their electrical properties were investigated by measuring their zeta-potential values. Results. In the biorelevant media, vesicles (either unilamellar or multilamellar) and bilayer fragments are visualized. Occasionally, vesicles with an internal deformed structure are recognized, suggesting surface tension or uneven lateral stress. Visualization studies of the intermediate colloidal phases produced during digestion of a SNEDDS using the in vitro lipolysis model revealed the formation of similar structures as previously reported. The zeta-potential of the media was negatively charged and decreased from -23 to -35 mV with increasing surfactant/lipid load. Lower zeta-potential values (-16 mV) obtained for the structures formed during the lipid hydrolysis of the SNEDDS were probably due to the presence of calcium, which shields the surface, thereby reducing the charge. Conclusions. The diversity of these vesicles in terms of size, lamellarity, and internal organization advocate their important role during lipid digestion in the gastrointestinal milieu.}},
author = {{Fatouros, Dimitrios G. and Walrand, Isabelle and Bergenståhl, Björn and Mullertz, Anette}},
issn = {{1573-904X}},
keywords = {{multilamellar vesicles; micelles; lipolytic products; in vitro digestion lipolysis model; microscopy; cryogenic transmission electron; zeta-potential; biorelevant media; unilamellar; systems; self-nanoemulsifying drug delivery}},
language = {{eng}},
number = {{2}},
pages = {{361--374}},
publisher = {{Springer}},
series = {{Pharmaceutical Research}},
title = {{Colloidal Structures in Media Simulating Intestinal Fed State Conditions with and Without Lipolysis Products}},
url = {{http://dx.doi.org/10.1007/s11095-008-9750-9}},
doi = {{10.1007/s11095-008-9750-9}},
volume = {{26}},
year = {{2009}},
}