CD11b(+)Ly6C(++)Ly6G(-) cells show distinct function in mice with chronic inflammation or tumor burden
(2012) In BMC Immunology 13.- Abstract
- Background: S100A9 has been shown to be important for the function of so called Myeloid Derived Suppressor Cells (MDSC). Cells with a similar phenotype are also involved in pro-inflammatory processes, and we therefore wanted to investigate the gene expression and function of these cells in animals that were either subjected to chronic inflammation, or inoculated with tumors. Methods: CD11b(+)Ly6C(++) and Ly6G(+) cells were isolated from spleen, tumor tissue or inflammatory granulomas. S100A9, Arginase 1 and iNOS gene expression in the various CD11b(+) cell populations was analyzed using Q-PCR. The suppressive activity of the CD11b(+) cell populations from different donors was studied in co-culture experiments. Results: S100A9 was shown to... (More)
- Background: S100A9 has been shown to be important for the function of so called Myeloid Derived Suppressor Cells (MDSC). Cells with a similar phenotype are also involved in pro-inflammatory processes, and we therefore wanted to investigate the gene expression and function of these cells in animals that were either subjected to chronic inflammation, or inoculated with tumors. Methods: CD11b(+)Ly6C(++) and Ly6G(+) cells were isolated from spleen, tumor tissue or inflammatory granulomas. S100A9, Arginase 1 and iNOS gene expression in the various CD11b(+) cell populations was analyzed using Q-PCR. The suppressive activity of the CD11b(+) cell populations from different donors was studied in co-culture experiments. Results: S100A9 was shown to be expressed mainly in splenic CD11b(+)Ly6C(+)G(+) cells both at the RNA and protein level. Arginase I and iNOS expression could be detected in both CD11b(+)Ly6C(+)Ly6G(+) and CD11b(+)Ly6C(+)G(-)/C(++)G(-) derived from tumors or a site of chronic inflammation, but was very low in the same cell populations isolated from the spleen. CD11b(+) cells isolated from mice with peritoneal chronic inflammation were able to stimulate T lymphocytes, while CD11b(+) cells from mice with peritoneal tumors suppressed T cell growth. Conclusion: An identical CD11b(+)Ly6C(++)G(-) cell population appears to have the ability to adopt immune stimulatory or immune suppressive functions dependent on the presence of a local inflammatory or tumor microenvironment. Thus, there is a functional plasticity in the CD11b(+)Ly6C(++)G(-) cell population that cannot be distinguished with the current molecular markers. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3481637
- author
- Källberg, Eva LU ; Stenström, Martin LU ; Liberg, David LU ; Ivars, Fredrik LU and Leanderson, Tomas LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Tumor, Inflammation, Myeloid cells, T cells, Suppression
- in
- BMC Immunology
- volume
- 13
- article number
- 69
- publisher
- BioMed Central (BMC)
- external identifiers
-
- wos:000313523100001
- scopus:84870892487
- pmid:23234398
- ISSN
- 1471-2172
- DOI
- 10.1186/1471-2172-13-69
- language
- English
- LU publication?
- yes
- id
- be3a06da-2d46-4913-b785-548eae826b5e (old id 3481637)
- date added to LUP
- 2016-04-01 13:46:12
- date last changed
- 2022-01-27 20:59:29
@article{be3a06da-2d46-4913-b785-548eae826b5e, abstract = {{Background: S100A9 has been shown to be important for the function of so called Myeloid Derived Suppressor Cells (MDSC). Cells with a similar phenotype are also involved in pro-inflammatory processes, and we therefore wanted to investigate the gene expression and function of these cells in animals that were either subjected to chronic inflammation, or inoculated with tumors. Methods: CD11b(+)Ly6C(++) and Ly6G(+) cells were isolated from spleen, tumor tissue or inflammatory granulomas. S100A9, Arginase 1 and iNOS gene expression in the various CD11b(+) cell populations was analyzed using Q-PCR. The suppressive activity of the CD11b(+) cell populations from different donors was studied in co-culture experiments. Results: S100A9 was shown to be expressed mainly in splenic CD11b(+)Ly6C(+)G(+) cells both at the RNA and protein level. Arginase I and iNOS expression could be detected in both CD11b(+)Ly6C(+)Ly6G(+) and CD11b(+)Ly6C(+)G(-)/C(++)G(-) derived from tumors or a site of chronic inflammation, but was very low in the same cell populations isolated from the spleen. CD11b(+) cells isolated from mice with peritoneal chronic inflammation were able to stimulate T lymphocytes, while CD11b(+) cells from mice with peritoneal tumors suppressed T cell growth. Conclusion: An identical CD11b(+)Ly6C(++)G(-) cell population appears to have the ability to adopt immune stimulatory or immune suppressive functions dependent on the presence of a local inflammatory or tumor microenvironment. Thus, there is a functional plasticity in the CD11b(+)Ly6C(++)G(-) cell population that cannot be distinguished with the current molecular markers.}}, author = {{Källberg, Eva and Stenström, Martin and Liberg, David and Ivars, Fredrik and Leanderson, Tomas}}, issn = {{1471-2172}}, keywords = {{Tumor; Inflammation; Myeloid cells; T cells; Suppression}}, language = {{eng}}, publisher = {{BioMed Central (BMC)}}, series = {{BMC Immunology}}, title = {{CD11b(+)Ly6C(++)Ly6G(-) cells show distinct function in mice with chronic inflammation or tumor burden}}, url = {{https://lup.lub.lu.se/search/files/3577601/3805904.pdf}}, doi = {{10.1186/1471-2172-13-69}}, volume = {{13}}, year = {{2012}}, }