Reduction of proliferating non-neuronal cells in dried nerve segments partly impairs nerve regeneration
Widerberg, Annika LU and Dahlin, Lars LU
(2000)
In NeuroReport
11(12).
p.2707-2707
- Abstract
- To determine whether drying of nerve grafts can affect axonal outgrowth and proliferation of non-neuronal cells, nerve segments dried for 0-60 min were used as nerve grafts to bridge a gap in transected rat sciatic nerves. Axonal outgrowth was measured by pinch reflex test and confirmed by immunocytochemical staining of neurofilaments. The proliferation of non-neuronal cells was measured by incorporation of BrdU in the dried nerve segments. Drying of the nerve segment for 60 min reduced the length of axonal outgrowth to 66 and 76% 3 and 6 days, respectively, after the grafting procedure. At that time point the number of proliferating cells was reduced by 51%. It is concluded that the number of proliferating non-neuronal cells is reduced in... (More)
- To determine whether drying of nerve grafts can affect axonal outgrowth and proliferation of non-neuronal cells, nerve segments dried for 0-60 min were used as nerve grafts to bridge a gap in transected rat sciatic nerves. Axonal outgrowth was measured by pinch reflex test and confirmed by immunocytochemical staining of neurofilaments. The proliferation of non-neuronal cells was measured by incorporation of BrdU in the dried nerve segments. Drying of the nerve segment for 60 min reduced the length of axonal outgrowth to 66 and 76% 3 and 6 days, respectively, after the grafting procedure. At that time point the number of proliferating cells was reduced by 51%. It is concluded that the number of proliferating non-neuronal cells is reduced in dried nerve segments which only partly impairs axonal outgrowth. Factors other than Schwann cells are probably important for an optimal mileue for regeneration in nerve grafts. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1117725
- author
- Widerberg, Annika
LU
and Dahlin, Lars
LU
- organization
- publishing date
- 2000
- type
- Contribution to journal
- publication status
- published
- subject
- in
- NeuroReport
- volume
- 11
- issue
- 12
- pages
- 2707 - 2707
- publisher
- Lippincott Williams & Wilkins
- external identifiers
-
- pmid:10976948
- scopus:0034698976
- ISSN
- 1473-558X
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Hand Surgery Research Group (013241910), Reconstructive Surgery (013240300)
- id
- be87604d-82d5-4c5a-8088-566e30ef6d19 (old id 1117725)
- date added to LUP
- 2016-04-01 12:25:49
- date last changed
- 2022-01-27 03:38:30
@article{be87604d-82d5-4c5a-8088-566e30ef6d19,
abstract = {{To determine whether drying of nerve grafts can affect axonal outgrowth and proliferation of non-neuronal cells, nerve segments dried for 0-60 min were used as nerve grafts to bridge a gap in transected rat sciatic nerves. Axonal outgrowth was measured by pinch reflex test and confirmed by immunocytochemical staining of neurofilaments. The proliferation of non-neuronal cells was measured by incorporation of BrdU in the dried nerve segments. Drying of the nerve segment for 60 min reduced the length of axonal outgrowth to 66 and 76% 3 and 6 days, respectively, after the grafting procedure. At that time point the number of proliferating cells was reduced by 51%. It is concluded that the number of proliferating non-neuronal cells is reduced in dried nerve segments which only partly impairs axonal outgrowth. Factors other than Schwann cells are probably important for an optimal mileue for regeneration in nerve grafts.}},
author = {{Widerberg, Annika and Dahlin, Lars}},
issn = {{1473-558X}},
language = {{eng}},
number = {{12}},
pages = {{2707--2707}},
publisher = {{Lippincott Williams & Wilkins}},
series = {{NeuroReport}},
title = {{Reduction of proliferating non-neuronal cells in dried nerve segments partly impairs nerve regeneration}},
volume = {{11}},
year = {{2000}},
}