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Histologic characterization of stem cells in mammary epithelium and stroma

Isfoss, Björn LU (2017)
Abstract (Swedish)
It is unknown whether native cells in benign breast tissue mediate risk of cancer.
We therefore aimed to identify cell types in benign human mammary tissue
immunohistologically using markers of stemness and differentiation, and to
investigate their possible associations with clinical risk factors for breast cancer.
We found that cells that are integrated in benign epithelium and are
immunoreactive for the stem cell marker ALDH1 are negative for markers of
glandular differentiation, proliferation, and estrogen receptor. High numbers of
these cells were associated with the risk factors family history of breast cancer,
BRCA1/2 carrier status, low parity, and hormone use.
Stromal ALDH1+ cells are either... (More)
It is unknown whether native cells in benign breast tissue mediate risk of cancer.
We therefore aimed to identify cell types in benign human mammary tissue
immunohistologically using markers of stemness and differentiation, and to
investigate their possible associations with clinical risk factors for breast cancer.
We found that cells that are integrated in benign epithelium and are
immunoreactive for the stem cell marker ALDH1 are negative for markers of
glandular differentiation, proliferation, and estrogen receptor. High numbers of
these cells were associated with the risk factors family history of breast cancer,
BRCA1/2 carrier status, low parity, and hormone use.
Stromal ALDH1+ cells are either spindle-shaped/polygonal (s/p) or round/oval
(r/o), and the latter are positive for the contractile protein marker SMMHC.
ALDH1+ s/p cells are positive for the stellate cell marker vinculin. Low numbers
of these cells were associated with family history, and when negative for the stem
cell marker CD44 they were associated with nulliparity. Low numbers of
ALDH1+ CD44+ CD24– r/o cells were associated with family history. ALDH1+
r/o cells were positively or negatively associated with low parity depending on the
patients' BRCA1/2 status.
High numbers of r/o cells that are positive for the mesenchymal stem cell
marker SSEA3 were associated with low parity, family history, and hormone use
after menopause.
This study describes novel stromal cells in breast that were associated with risk
factors for breast cancer. If confirmed in independent cohorts, they may be used
for stratification of women with regard to risk of breast cancer. (Less)
Please use this url to cite or link to this publication:
author
supervisor
opponent
  • professor Isola, Jorma, Tampere, Finland
organization
publishing date
type
Thesis
publication status
published
pages
59 pages
publisher
Lund University, Faculty of Medicine
defense location
Segerfalksalen, Biomedicinskt centrum, Sölvegatan 17, Lund
defense date
2017-04-28 09:00
ISBN
978-91-7619-439-3
language
English
LU publication?
yes
id
beb356f5-9799-4e4b-aaba-02fba13fae93
date added to LUP
2017-04-07 22:41:25
date last changed
2017-05-02 10:40:27
@phdthesis{beb356f5-9799-4e4b-aaba-02fba13fae93,
  abstract     = {It is unknown whether native cells in benign breast tissue mediate risk of cancer.<br/>We therefore aimed to identify cell types in benign human mammary tissue<br/>immunohistologically using markers of stemness and differentiation, and to<br/>investigate their possible associations with clinical risk factors for breast cancer.<br/>We found that cells that are integrated in benign epithelium and are<br/>immunoreactive for the stem cell marker ALDH1 are negative for markers of<br/>glandular differentiation, proliferation, and estrogen receptor. High numbers of<br/>these cells were associated with the risk factors family history of breast cancer,<br/>BRCA1/2 carrier status, low parity, and hormone use.<br/>Stromal ALDH1+ cells are either spindle-shaped/polygonal (s/p) or round/oval<br/>(r/o), and the latter are positive for the contractile protein marker SMMHC.<br/>ALDH1+ s/p cells are positive for the stellate cell marker vinculin. Low numbers<br/>of these cells were associated with family history, and when negative for the stem<br/>cell marker CD44 they were associated with nulliparity. Low numbers of<br/>ALDH1+ CD44+ CD24– r/o cells were associated with family history. ALDH1+<br/>r/o cells were positively or negatively associated with low parity depending on the<br/>patients' BRCA1/2 status.<br/>High numbers of r/o cells that are positive for the mesenchymal stem cell<br/>marker SSEA3 were associated with low parity, family history, and hormone use<br/>after menopause.<br/>This study describes novel stromal cells in breast that were associated with risk<br/>factors for breast cancer. If confirmed in independent cohorts, they may be used<br/>for stratification of women with regard to risk of breast cancer.},
  author       = {Isfoss, Björn},
  isbn         = {978-91-7619-439-3},
  language     = {eng},
  pages        = {59},
  publisher    = {Lund University, Faculty of Medicine},
  school       = {Lund University},
  title        = {Histologic characterization of stem cells in mammary epithelium and stroma},
  year         = {2017},
}