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The absence of aldehyde dehydrogenase 1 A1-positive cells in benign mammary stroma is associated with risk factors for breast cancer

Isfoss, Björn Logi LU ; Holmqvist, Bo LU ; Jernström, Helena LU ; Alm, Per LU and Olsson, Håkan LU (2016) In Breast Cancer: Targets and Therapy 8. p.117-124
Abstract

In this study, aldehyde dehydrogenase 1 (ALDH1)-expressing cells in stroma of histologically normal breast tissue from premenopausal women were investigated in situ regarding cellular morphology, cell distribution, and relation to the additional stem cell markers, CD44 (+) and CD24 (-). These results were correlated with hormonal and genetic risk factors for breast cancer. Triple immunofluorescence labeling was performed on tissues from premenopausal women with a family history of breast cancer, and breast reduction specimens from premenopausal women with no family history of breast cancer were used as a control group. The majority of ALDH1-immunoreactive cells in stroma were spindle-shaped or polygonal, and such cells that were... (More)

In this study, aldehyde dehydrogenase 1 (ALDH1)-expressing cells in stroma of histologically normal breast tissue from premenopausal women were investigated in situ regarding cellular morphology, cell distribution, and relation to the additional stem cell markers, CD44 (+) and CD24 (-). These results were correlated with hormonal and genetic risk factors for breast cancer. Triple immunofluorescence labeling was performed on tissues from premenopausal women with a family history of breast cancer, and breast reduction specimens from premenopausal women with no family history of breast cancer were used as a control group. The majority of ALDH1-immunoreactive cells in stroma were spindle-shaped or polygonal, and such cells that were CD44- and CD24- were absent in the breast stroma of a significantly larger number of nulliparous than parous women. A less common morphological type of ALDH1-positive cells in stroma was round or oval in shape, and such cells that were CD44+ and CD24- were absent in a significant number of women with a family history of breast cancer. The CD44+/CD24- immunophenotype is consistent with stem cells, and the round/oval morphology suggests mesenchymal cells. This study demonstrates that there are two morphologically distinct types of ALDH1-positive cells in histologically benign mammary stroma, and the absence of these cells is correlated with clinical risk factors for breast cancer in premenopausal women.

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organization
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type
Contribution to journal
publication status
published
subject
keywords
BRCA1 gene, Human, Immunohistochemistry, Mammary glands, Neoplasms, Stem cells
in
Breast Cancer: Targets and Therapy
volume
8
pages
8 pages
publisher
Dove Medical Press Ltd.
external identifiers
  • scopus:84975270486
  • wos:000376621500002
DOI
10.2147/BCTT.S106097
language
English
LU publication?
yes
id
33536639-f998-4674-8bc4-96a6d97f9e50
date added to LUP
2016-07-06 14:34:20
date last changed
2017-01-01 08:29:54
@article{33536639-f998-4674-8bc4-96a6d97f9e50,
  abstract     = {<p>In this study, aldehyde dehydrogenase 1 (ALDH1)-expressing cells in stroma of histologically normal breast tissue from premenopausal women were investigated in situ regarding cellular morphology, cell distribution, and relation to the additional stem cell markers, CD44 (+) and CD24 (-). These results were correlated with hormonal and genetic risk factors for breast cancer. Triple immunofluorescence labeling was performed on tissues from premenopausal women with a family history of breast cancer, and breast reduction specimens from premenopausal women with no family history of breast cancer were used as a control group. The majority of ALDH1-immunoreactive cells in stroma were spindle-shaped or polygonal, and such cells that were CD44<sup>-</sup> and CD24<sup>-</sup> were absent in the breast stroma of a significantly larger number of nulliparous than parous women. A less common morphological type of ALDH1-positive cells in stroma was round or oval in shape, and such cells that were CD44<sup>+</sup> and CD24<sup>-</sup> were absent in a significant number of women with a family history of breast cancer. The CD44<sup>+</sup>/CD24<sup>-</sup> immunophenotype is consistent with stem cells, and the round/oval morphology suggests mesenchymal cells. This study demonstrates that there are two morphologically distinct types of ALDH1-positive cells in histologically benign mammary stroma, and the absence of these cells is correlated with clinical risk factors for breast cancer in premenopausal women.</p>},
  author       = {Isfoss, Björn Logi and Holmqvist, Bo and Jernström, Helena and Alm, Per and Olsson, Håkan},
  keyword      = {BRCA1 gene,Human,Immunohistochemistry,Mammary glands,Neoplasms,Stem cells},
  language     = {eng},
  month        = {05},
  pages        = {117--124},
  publisher    = {Dove Medical Press Ltd.},
  series       = {Breast Cancer: Targets and Therapy},
  title        = {The absence of aldehyde dehydrogenase 1 A1-positive cells in benign mammary stroma is associated with risk factors for breast cancer},
  url          = {http://dx.doi.org/10.2147/BCTT.S106097},
  volume       = {8},
  year         = {2016},
}