The absence of aldehyde dehydrogenase 1 A1-positive cells in benign mammary stroma is associated with risk factors for breast cancer
Isfoss, Björn Logi LU ; Holmqvist, Bo LU ; Jernström, Helena LU ; Alm, Per LU and Olsson, Håkan LU
(2016)
In Breast Cancer: Targets and Therapy
8.
p.117-124
- Abstract
In this study, aldehyde dehydrogenase 1 (ALDH1)-expressing cells in stroma of histologically normal breast tissue from premenopausal women were investigated in situ regarding cellular morphology, cell distribution, and relation to the additional stem cell markers, CD44 (+) and CD24 (-). These results were correlated with hormonal and genetic risk factors for breast cancer. Triple immunofluorescence labeling was performed on tissues from premenopausal women with a family history of breast cancer, and breast reduction specimens from premenopausal women with no family history of breast cancer were used as a control group. The majority of ALDH1-immunoreactive cells in stroma were spindle-shaped or polygonal, and such cells that were... (More)
In this study, aldehyde dehydrogenase 1 (ALDH1)-expressing cells in stroma of histologically normal breast tissue from premenopausal women were investigated in situ regarding cellular morphology, cell distribution, and relation to the additional stem cell markers, CD44 (+) and CD24 (-). These results were correlated with hormonal and genetic risk factors for breast cancer. Triple immunofluorescence labeling was performed on tissues from premenopausal women with a family history of breast cancer, and breast reduction specimens from premenopausal women with no family history of breast cancer were used as a control group. The majority of ALDH1-immunoreactive cells in stroma were spindle-shaped or polygonal, and such cells that were CD44- and CD24- were absent in the breast stroma of a significantly larger number of nulliparous than parous women. A less common morphological type of ALDH1-positive cells in stroma was round or oval in shape, and such cells that were CD44+ and CD24- were absent in a significant number of women with a family history of breast cancer. The CD44+/CD24- immunophenotype is consistent with stem cells, and the round/oval morphology suggests mesenchymal cells. This study demonstrates that there are two morphologically distinct types of ALDH1-positive cells in histologically benign mammary stroma, and the absence of these cells is correlated with clinical risk factors for breast cancer in premenopausal women.
(Less)
- author
- Isfoss, Björn Logi
LU
; Holmqvist, Bo
LU
; Jernström, Helena
LU
; Alm, Per
LU
and Olsson, Håkan
LU
- organization
- publishing date
- 2016-05-27
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- BRCA1 gene, Human, Immunohistochemistry, Mammary glands, Neoplasms, Stem cells
- in
- Breast Cancer: Targets and Therapy
- volume
- 8
- pages
- 8 pages
- publisher
- Dove Medical Press Ltd.
- external identifiers
-
- scopus:84975270486
- pmid:27313475
- wos:000376621500002
- ISSN
- 1179-1314
- DOI
- 10.2147/BCTT.S106097
- language
- English
- LU publication?
- yes
- id
- 33536639-f998-4674-8bc4-96a6d97f9e50
- date added to LUP
- 2016-07-06 14:34:20
- date last changed
- 2024-06-28 12:08:47
@article{33536639-f998-4674-8bc4-96a6d97f9e50,
abstract = {{<p>In this study, aldehyde dehydrogenase 1 (ALDH1)-expressing cells in stroma of histologically normal breast tissue from premenopausal women were investigated in situ regarding cellular morphology, cell distribution, and relation to the additional stem cell markers, CD44 (+) and CD24 (-). These results were correlated with hormonal and genetic risk factors for breast cancer. Triple immunofluorescence labeling was performed on tissues from premenopausal women with a family history of breast cancer, and breast reduction specimens from premenopausal women with no family history of breast cancer were used as a control group. The majority of ALDH1-immunoreactive cells in stroma were spindle-shaped or polygonal, and such cells that were CD44<sup>-</sup> and CD24<sup>-</sup> were absent in the breast stroma of a significantly larger number of nulliparous than parous women. A less common morphological type of ALDH1-positive cells in stroma was round or oval in shape, and such cells that were CD44<sup>+</sup> and CD24<sup>-</sup> were absent in a significant number of women with a family history of breast cancer. The CD44<sup>+</sup>/CD24<sup>-</sup> immunophenotype is consistent with stem cells, and the round/oval morphology suggests mesenchymal cells. This study demonstrates that there are two morphologically distinct types of ALDH1-positive cells in histologically benign mammary stroma, and the absence of these cells is correlated with clinical risk factors for breast cancer in premenopausal women.</p>}},
author = {{Isfoss, Björn Logi and Holmqvist, Bo and Jernström, Helena and Alm, Per and Olsson, Håkan}},
issn = {{1179-1314}},
keywords = {{BRCA1 gene; Human; Immunohistochemistry; Mammary glands; Neoplasms; Stem cells}},
language = {{eng}},
month = {{05}},
pages = {{117--124}},
publisher = {{Dove Medical Press Ltd.}},
series = {{Breast Cancer: Targets and Therapy}},
title = {{The absence of aldehyde dehydrogenase 1 A1-positive cells in benign mammary stroma is associated with risk factors for breast cancer}},
url = {{http://dx.doi.org/10.2147/BCTT.S106097}},
doi = {{10.2147/BCTT.S106097}},
volume = {{8}},
year = {{2016}},
}