‘Hot Spots’ of creatine kinase localization in brain : Cerebellum, hippocampus and choroid plexus
(1996) In Developmental Neuroscience 18(5-6). p.542-554- Abstract
Creatine kinase (CK) isoenzymes, with emphasis on the mitochondrial CK isoenzymes, were characterized and localized in chicken cerebellum. Chicken cerebellar extracts analyzed by two-dimensional gels, using anti-peptide antibodies specific for sarcomeric muscle-type mitochondrial CK (Mib-CK) and revealed the presence of a Mib-CK variant in avian cerebellum. This CK isoform was localized by immunofluorescence staining exclusively in the Purkinje neurons. The co-expression of this Mib-CK together with cytosolic muscle-type MM-CK, as observed in the same Purkinje neurons, may reflect the specific energy requirements associated with highly fluctuating Ca2+ levels (Ca2+ spiking) in these... (More)
Creatine kinase (CK) isoenzymes, with emphasis on the mitochondrial CK isoenzymes, were characterized and localized in chicken cerebellum. Chicken cerebellar extracts analyzed by two-dimensional gels, using anti-peptide antibodies specific for sarcomeric muscle-type mitochondrial CK (Mib-CK) and revealed the presence of a Mib-CK variant in avian cerebellum. This CK isoform was localized by immunofluorescence staining exclusively in the Purkinje neurons. The co-expression of this Mib-CK together with cytosolic muscle-type MM-CK, as observed in the same Purkinje neurons, may reflect the specific energy requirements associated with highly fluctuating Ca2+ levels (Ca2+ spiking) in these specialized neurons. Ubiquitous brain-type mitochondrial Mia-CK was found together with cytosolic BB-CK mainly in the glomeruli structures of the cerebellar granular layer. BB-CK, but much less so Mia-CK, however, was also very prominent in Bergmann glial cells of the molecular layer. Thus, an isoenzyme-specific differential localization of the two mitochondrial Mi-CK isoenzymes in the chicken cerebellum is demonstrated. Other hot spots of CK localization were the granule and pyramidal cells of the hippocampus in rat. There, a developmental stage-dependent immunofluorescence staining, especially with antibodies against Mia-CK, was noted. Epithelial cells of the choroid plexus were also highly enriched in CK. The possible implications of a CK/PCr circuit at these various cellular locations of the brain are discussed with respect to normal brain physiology and pathology.
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- author
- Kaldis, Philipp LU ; Hemmer, Wolfram ; Zanolla, Else ; Holtzman, David and Wallimann, Theo
- publishing date
- 1996-01-01
- type
- Contribution to journal
- publication status
- published
- keywords
- Bergmann glia, Brain energy metabolism, Cerebellum, Creatine kinase, Epithelial cells of choroid plexus, Granular and pyramidal cells of hippocampus, Immunofluorescence microscopy, Purkinje neurons
- in
- Developmental Neuroscience
- volume
- 18
- issue
- 5-6
- pages
- 542 - 554
- publisher
- Karger
- external identifiers
-
- pmid:8940630
- scopus:0029842655
- ISSN
- 0378-5866
- DOI
- 10.1159/000111452
- language
- English
- LU publication?
- no
- id
- bf531fd3-b519-46d3-ab33-253e34b0fac2
- date added to LUP
- 2019-09-18 14:37:34
- date last changed
- 2024-08-07 06:51:59
@article{bf531fd3-b519-46d3-ab33-253e34b0fac2, abstract = {{<p>Creatine kinase (CK) isoenzymes, with emphasis on the mitochondrial CK isoenzymes, were characterized and localized in chicken cerebellum. Chicken cerebellar extracts analyzed by two-dimensional gels, using anti-peptide antibodies specific for sarcomeric muscle-type mitochondrial CK (Mi<sub>b</sub>-CK) and revealed the presence of a Mi<sub>b</sub>-CK variant in avian cerebellum. This CK isoform was localized by immunofluorescence staining exclusively in the Purkinje neurons. The co-expression of this Mi<sub>b</sub>-CK together with cytosolic muscle-type MM-CK, as observed in the same Purkinje neurons, may reflect the specific energy requirements associated with highly fluctuating Ca<sup>2+</sup> levels (Ca<sup>2+</sup> spiking) in these specialized neurons. Ubiquitous brain-type mitochondrial Mi<sub>a</sub>-CK was found together with cytosolic BB-CK mainly in the glomeruli structures of the cerebellar granular layer. BB-CK, but much less so Mi<sub>a</sub>-CK, however, was also very prominent in Bergmann glial cells of the molecular layer. Thus, an isoenzyme-specific differential localization of the two mitochondrial Mi-CK isoenzymes in the chicken cerebellum is demonstrated. Other hot spots of CK localization were the granule and pyramidal cells of the hippocampus in rat. There, a developmental stage-dependent immunofluorescence staining, especially with antibodies against Mi<sub>a</sub>-CK, was noted. Epithelial cells of the choroid plexus were also highly enriched in CK. The possible implications of a CK/PCr circuit at these various cellular locations of the brain are discussed with respect to normal brain physiology and pathology.</p>}}, author = {{Kaldis, Philipp and Hemmer, Wolfram and Zanolla, Else and Holtzman, David and Wallimann, Theo}}, issn = {{0378-5866}}, keywords = {{Bergmann glia; Brain energy metabolism; Cerebellum; Creatine kinase; Epithelial cells of choroid plexus; Granular and pyramidal cells of hippocampus; Immunofluorescence microscopy; Purkinje neurons}}, language = {{eng}}, month = {{01}}, number = {{5-6}}, pages = {{542--554}}, publisher = {{Karger}}, series = {{Developmental Neuroscience}}, title = {{‘Hot Spots’ of creatine kinase localization in brain : Cerebellum, hippocampus and choroid plexus}}, url = {{http://dx.doi.org/10.1159/000111452}}, doi = {{10.1159/000111452}}, volume = {{18}}, year = {{1996}}, }