Reactions catalyzed by peg-modified αchymotrypsin in organic solvents. Influence of water content and degree of modification
Ljunger, Gudrun ; Adlercreutz, Patrick LU
and Mattiasson, Bo
LU
(1993)
In Biocatalysis and Biotransformation
7(4).
p.279-288
- Abstract
αChymotrypsin was modified with cyanuric chloride activated monomethoxypolyethylene glycol (MPEG) with molecular weights 1900 and 5000. Using the higher molecular weight MPEG a product that was soluble in benzene at moderate levels of modification was obtained, whereas with MPEG 1900 almost all the enzyme's amino groups had to be modified for dissolving the conjugate. The catalytic activity decreased with increasing degree of substitution. Apparent Vmax was considerably higher for the less modified enzyme preparation than for the more modified one, while Km,app stayed almost constant. The modified enzyme was used for peptide synthesis. The reaction was dependent on the content of dissolved water. Both... (More)
αChymotrypsin was modified with cyanuric chloride activated monomethoxypolyethylene glycol (MPEG) with molecular weights 1900 and 5000. Using the higher molecular weight MPEG a product that was soluble in benzene at moderate levels of modification was obtained, whereas with MPEG 1900 almost all the enzyme's amino groups had to be modified for dissolving the conjugate. The catalytic activity decreased with increasing degree of substitution. Apparent Vmax was considerably higher for the less modified enzyme preparation than for the more modified one, while Km,app stayed almost constant. The modified enzyme was used for peptide synthesis. The reaction was dependent on the content of dissolved water. Both Vmax,app and Km,app increased with increasing water content. It was possible to achieve a process with complete conversion of substrate to dipeptide.
(Less)
- author
- Ljunger, Gudrun
; Adlercreutz, Patrick
LU
and Mattiasson, Bo
LU
- organization
- publishing date
- 1993-01-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Organic media, Peptide synthesis, Polyethylene glycol
- in
- Biocatalysis and Biotransformation
- volume
- 7
- issue
- 4
- pages
- 10 pages
- publisher
- Taylor & Francis
- external identifiers
-
- scopus:2142788032
- ISSN
- 1024-2422
- DOI
- 10.3109/10242429308992100
- language
- English
- LU publication?
- yes
- id
- bf98c4f8-0392-46cd-8d8d-da37996a1c26
- date added to LUP
- 2019-06-22 09:15:51
- date last changed
- 2021-01-03 08:29:54
@article{bf98c4f8-0392-46cd-8d8d-da37996a1c26,
abstract = {{<p>αChymotrypsin was modified with cyanuric chloride activated monomethoxypolyethylene glycol (MPEG) with molecular weights 1900 and 5000. Using the higher molecular weight MPEG a product that was soluble in benzene at moderate levels of modification was obtained, whereas with MPEG 1900 almost all the enzyme's amino groups had to be modified for dissolving the conjugate. The catalytic activity decreased with increasing degree of substitution. Apparent V<sub>max</sub> was considerably higher for the less modified enzyme preparation than for the more modified one, while K<sub>m,app</sub> stayed almost constant. The modified enzyme was used for peptide synthesis. The reaction was dependent on the content of dissolved water. Both V<sub>max,app</sub> and K<sub>m,app</sub> increased with increasing water content. It was possible to achieve a process with complete conversion of substrate to dipeptide.</p>}},
author = {{Ljunger, Gudrun and Adlercreutz, Patrick and Mattiasson, Bo}},
issn = {{1024-2422}},
keywords = {{Organic media; Peptide synthesis; Polyethylene glycol}},
language = {{eng}},
month = {{01}},
number = {{4}},
pages = {{279--288}},
publisher = {{Taylor & Francis}},
series = {{Biocatalysis and Biotransformation}},
title = {{Reactions catalyzed by peg-modified αchymotrypsin in organic solvents. Influence of water content and degree of modification}},
url = {{http://dx.doi.org/10.3109/10242429308992100}},
doi = {{10.3109/10242429308992100}},
volume = {{7}},
year = {{1993}},
}