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Maintaining multipotent trunk neural crest stem cells as self-renewing crestospheres

Mohlin, Sofie LU orcid ; Kunttas, Ezgi ; Persson, Camilla U. LU ; Abdel-Haq, Reem ; Castillo, Aldo ; Murko, Christina ; Bronner, Marianne E. and Kerosuo, Laura (2019) In Developmental Biology 447(2). p.137-146
Abstract

Neural crest cells have broad migratory and differentiative ability that differs according to their axial level of origin. However, their transient nature has limited understanding of their stem cell and self-renewal properties. While an in vitro culture method has made it possible to maintain cranial neural crest cells as self-renewing multipotent crestospheres (Kerosuo et al., 2015), these same conditions failed to preserve trunk neural crest in a stem-like state. Here we optimize culture conditions for maintenance of avian trunk crestospheres, comprised of both neural crest stem and progenitor cells. Our trunk-derived crestospheres are multipotent and display self-renewal capacity over several weeks. Trunk crestospheres display... (More)

Neural crest cells have broad migratory and differentiative ability that differs according to their axial level of origin. However, their transient nature has limited understanding of their stem cell and self-renewal properties. While an in vitro culture method has made it possible to maintain cranial neural crest cells as self-renewing multipotent crestospheres (Kerosuo et al., 2015), these same conditions failed to preserve trunk neural crest in a stem-like state. Here we optimize culture conditions for maintenance of avian trunk crestospheres, comprised of both neural crest stem and progenitor cells. Our trunk-derived crestospheres are multipotent and display self-renewal capacity over several weeks. Trunk crestospheres display elevated expression of neural crest cell markers as compared to those characteristic of ventrolateral neural tube or mesodermal fates. Moreover, trunk crestospheres express increased levels of trunk neural crest-enriched markers as compared to cranial crestospheres. Finally, we use lentiviral transduction as a tool to manipulate gene expression in trunk crestospheres. Taken together, this method enables long-term in vitro maintenance and manipulation of multipotent trunk neural crest cells in a premigratory stem or early progenitor state. Trunk crestospheres are a valuable resource for probing mechanisms underlying neural crest stemness and lineage decisions as well as accompanying diseases.

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author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Crestospheres, Multipotency, self-renewal, Neural crest stem cells, Stem cell maintenance, Trunk neural crest
in
Developmental Biology
volume
447
issue
2
pages
137 - 146
publisher
Elsevier
external identifiers
  • pmid:30664880
  • scopus:85060227797
ISSN
0012-1606
DOI
10.1016/j.ydbio.2019.01.010
language
English
LU publication?
yes
id
bfaea09a-11cc-43f5-ba18-24f9639a7f3f
date added to LUP
2019-02-01 11:09:09
date last changed
2024-01-15 13:11:58
@article{bfaea09a-11cc-43f5-ba18-24f9639a7f3f,
  abstract     = {{<p>Neural crest cells have broad migratory and differentiative ability that differs according to their axial level of origin. However, their transient nature has limited understanding of their stem cell and self-renewal properties. While an in vitro culture method has made it possible to maintain cranial neural crest cells as self-renewing multipotent crestospheres (Kerosuo et al., 2015), these same conditions failed to preserve trunk neural crest in a stem-like state. Here we optimize culture conditions for maintenance of avian trunk crestospheres, comprised of both neural crest stem and progenitor cells. Our trunk-derived crestospheres are multipotent and display self-renewal capacity over several weeks. Trunk crestospheres display elevated expression of neural crest cell markers as compared to those characteristic of ventrolateral neural tube or mesodermal fates. Moreover, trunk crestospheres express increased levels of trunk neural crest-enriched markers as compared to cranial crestospheres. Finally, we use lentiviral transduction as a tool to manipulate gene expression in trunk crestospheres. Taken together, this method enables long-term in vitro maintenance and manipulation of multipotent trunk neural crest cells in a premigratory stem or early progenitor state. Trunk crestospheres are a valuable resource for probing mechanisms underlying neural crest stemness and lineage decisions as well as accompanying diseases.</p>}},
  author       = {{Mohlin, Sofie and Kunttas, Ezgi and Persson, Camilla U. and Abdel-Haq, Reem and Castillo, Aldo and Murko, Christina and Bronner, Marianne E. and Kerosuo, Laura}},
  issn         = {{0012-1606}},
  keywords     = {{Crestospheres; Multipotency, self-renewal; Neural crest stem cells; Stem cell maintenance; Trunk neural crest}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{2}},
  pages        = {{137--146}},
  publisher    = {{Elsevier}},
  series       = {{Developmental Biology}},
  title        = {{Maintaining multipotent trunk neural crest stem cells as self-renewing crestospheres}},
  url          = {{http://dx.doi.org/10.1016/j.ydbio.2019.01.010}},
  doi          = {{10.1016/j.ydbio.2019.01.010}},
  volume       = {{447}},
  year         = {{2019}},
}