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Solid-phase extraction of the alcohol abuse biomarker phosphatidylethanol using newly synthesized polymeric sorbent materials containing quaternary heterocyclic groups

Nogueira Duarte, Mariana LU ; Jagadeesan, Kishore Kumar LU ; Billing, Johan LU ; Yilmaz, Ecevit LU ; Laurell, Thomas LU and Ekström, Simon LU (2017) In Journal of Chromatography A 1519. p.1-8
Abstract

Phosphatidylethanol (PEth) is an interesting biomarker finding increased use for detecting long term alcohol abuse with high specificity and sensitivity. Prior to detection, sample preparation is an unavoidable step in the work-flow of PEth analysis and new protocols may facilitate it. Solid-phase extraction (SPE) is a versatile sample preparation method widely spread in biomedical laboratories due to its simplicity of use and the possibility of automation. In this work, SPE was used for the first time to directly extract PEth from spiked human plasma and spiked human blood. A library of polymeric SPE materials with different surface functionalities was screened for PEth extraction in order to identify the surface characteristics that... (More)

Phosphatidylethanol (PEth) is an interesting biomarker finding increased use for detecting long term alcohol abuse with high specificity and sensitivity. Prior to detection, sample preparation is an unavoidable step in the work-flow of PEth analysis and new protocols may facilitate it. Solid-phase extraction (SPE) is a versatile sample preparation method widely spread in biomedical laboratories due to its simplicity of use and the possibility of automation. In this work, SPE was used for the first time to directly extract PEth from spiked human plasma and spiked human blood. A library of polymeric SPE materials with different surface functionalities was screened for PEth extraction in order to identify the surface characteristics that control PEth retention and recovery. The plasma samples were diluted 1:10 (v/v) in water and spiked at different concentrations ranging from 0.3 to 5 μM. The library of SPE materials was then evaluated using the proposed SPE method and detection was done by LC–MS/MS. One SPE material efficiently retained and recovered PEth from spiked human plasma. With this insight, four new SPE materials were formulated and synthesized based on the surface characteristics of the best SPE material found in the first screening. These new materials were tested with spiked human blood, to better mimic a real clinical sample. All the newly synthetized materials outperformed the pre-existing commercially available materials. Recovery values for the new SPE materials were found between 29.5% and 48.6% for the extraction of PEth in spiked blood. A material based on quaternized 1-vinylimidazole with a poly(trimethylolpropane trimethacrylate) backbone was found suitable for PEth extraction in spiked blood showing the highest analyte recovery in this experiment, 48.6% ± 6.4%.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Ion-exchangers, Phosphatidylethanol, Polymer adsorbents, Screening, Solid-phase extraction
in
Journal of Chromatography A
volume
1519
pages
8 pages
publisher
Elsevier
external identifiers
  • scopus:85030837866
  • wos:000413376700001
ISSN
0021-9673
DOI
10.1016/j.chroma.2017.08.051
language
English
LU publication?
yes
id
bfc48739-77ab-4bae-b84f-8ddc32b793b6
date added to LUP
2017-10-18 07:54:27
date last changed
2018-01-16 13:23:32
@article{bfc48739-77ab-4bae-b84f-8ddc32b793b6,
  abstract     = {<p>Phosphatidylethanol (PEth) is an interesting biomarker finding increased use for detecting long term alcohol abuse with high specificity and sensitivity. Prior to detection, sample preparation is an unavoidable step in the work-flow of PEth analysis and new protocols may facilitate it. Solid-phase extraction (SPE) is a versatile sample preparation method widely spread in biomedical laboratories due to its simplicity of use and the possibility of automation. In this work, SPE was used for the first time to directly extract PEth from spiked human plasma and spiked human blood. A library of polymeric SPE materials with different surface functionalities was screened for PEth extraction in order to identify the surface characteristics that control PEth retention and recovery. The plasma samples were diluted 1:10 (v/v) in water and spiked at different concentrations ranging from 0.3 to 5 μM. The library of SPE materials was then evaluated using the proposed SPE method and detection was done by LC–MS/MS. One SPE material efficiently retained and recovered PEth from spiked human plasma. With this insight, four new SPE materials were formulated and synthesized based on the surface characteristics of the best SPE material found in the first screening. These new materials were tested with spiked human blood, to better mimic a real clinical sample. All the newly synthetized materials outperformed the pre-existing commercially available materials. Recovery values for the new SPE materials were found between 29.5% and 48.6% for the extraction of PEth in spiked blood. A material based on quaternized 1-vinylimidazole with a poly(trimethylolpropane trimethacrylate) backbone was found suitable for PEth extraction in spiked blood showing the highest analyte recovery in this experiment, 48.6% ± 6.4%.</p>},
  author       = {Nogueira Duarte, Mariana and Jagadeesan, Kishore Kumar and Billing, Johan and Yilmaz, Ecevit and Laurell, Thomas and Ekström, Simon},
  issn         = {0021-9673},
  keyword      = {Ion-exchangers,Phosphatidylethanol,Polymer adsorbents,Screening,Solid-phase extraction},
  language     = {eng},
  month        = {10},
  pages        = {1--8},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography A},
  title        = {Solid-phase extraction of the alcohol abuse biomarker phosphatidylethanol using newly synthesized polymeric sorbent materials containing quaternary heterocyclic groups},
  url          = {http://dx.doi.org/10.1016/j.chroma.2017.08.051},
  volume       = {1519},
  year         = {2017},
}