Measurements of ATP in forest humus
(1991) In Soil Biology & Biochemistry 23(6). p.501-506- Abstract
- The measurement of ATP as an estimate of microbial biomass C in humus was studied. Three different acid extractants, H2SO4, TCA and H3PO4 were compared, as well as two different buffers, Tris and arsenate. No difference in extraction efficiency was found when the light output was converted to ATP content using internal ATP standards, but the different extraction agents and buffers influenced the light output. Several pretreatments and storage conditions were investigated. Incubation at 25°C for 1–7 days gave ca 50% higher ATP values than incubation at 4°C. Sieving did not affect the result. For long-term storage of humus, 4°C is recommended instead of freezing, since freezing reduced the ATP content to ca 80% of the amount found in humus... (More)
- The measurement of ATP as an estimate of microbial biomass C in humus was studied. Three different acid extractants, H2SO4, TCA and H3PO4 were compared, as well as two different buffers, Tris and arsenate. No difference in extraction efficiency was found when the light output was converted to ATP content using internal ATP standards, but the different extraction agents and buffers influenced the light output. Several pretreatments and storage conditions were investigated. Incubation at 25°C for 1–7 days gave ca 50% higher ATP values than incubation at 4°C. Sieving did not affect the result. For long-term storage of humus, 4°C is recommended instead of freezing, since freezing reduced the ATP content to ca 80% of the amount found in humus stored at 4°C after 2 months. Freezing of the humus extract resulted in even larger losses of ATP. Humus from eight coniferous forests in Sweden was investigated with respect to ATP content and microbial biomass, estimated with the substrate induced respiration rate method (SIR). A close linear relationship (r = 0.95) was found between the two types of measurement. Calculated from this relationship the amount of ATP (at 25°C) was 3.2μmg−1 biomass C. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/8046965
- author
- Arnebrant, Kristina LU and Bååth, Erland LU
- organization
- publishing date
- 1991
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Soil Biology & Biochemistry
- volume
- 23
- issue
- 6
- pages
- 501 - 506
- publisher
- Elsevier
- external identifiers
-
- scopus:0000471727
- ISSN
- 0038-0717
- DOI
- 10.1016/0038-0717(91)90106-T
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Microbial Ecology (Closed 2011) (011008001), Lund University Libraries (016520000), MEMEG (432112240)
- id
- c0740956-379a-4277-a23a-e5530d99e338 (old id 8046965)
- date added to LUP
- 2016-04-01 16:19:25
- date last changed
- 2021-01-03 06:31:03
@article{c0740956-379a-4277-a23a-e5530d99e338, abstract = {{The measurement of ATP as an estimate of microbial biomass C in humus was studied. Three different acid extractants, H2SO4, TCA and H3PO4 were compared, as well as two different buffers, Tris and arsenate. No difference in extraction efficiency was found when the light output was converted to ATP content using internal ATP standards, but the different extraction agents and buffers influenced the light output. Several pretreatments and storage conditions were investigated. Incubation at 25°C for 1–7 days gave ca 50% higher ATP values than incubation at 4°C. Sieving did not affect the result. For long-term storage of humus, 4°C is recommended instead of freezing, since freezing reduced the ATP content to ca 80% of the amount found in humus stored at 4°C after 2 months. Freezing of the humus extract resulted in even larger losses of ATP. Humus from eight coniferous forests in Sweden was investigated with respect to ATP content and microbial biomass, estimated with the substrate induced respiration rate method (SIR). A close linear relationship (r = 0.95) was found between the two types of measurement. Calculated from this relationship the amount of ATP (at 25°C) was 3.2μmg−1 biomass C.}}, author = {{Arnebrant, Kristina and Bååth, Erland}}, issn = {{0038-0717}}, language = {{eng}}, number = {{6}}, pages = {{501--506}}, publisher = {{Elsevier}}, series = {{Soil Biology & Biochemistry}}, title = {{Measurements of ATP in forest humus}}, url = {{http://dx.doi.org/10.1016/0038-0717(91)90106-T}}, doi = {{10.1016/0038-0717(91)90106-T}}, volume = {{23}}, year = {{1991}}, }