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An integrated acousto- and dielectrophoresis device for tumor cell separation, concentration, and single-cell trapping

Antfolk, M. LU ; Kim, S. H.; Saori, K.; Kaneda, Shohei; Fujii, T. and Laurell, T. LU (2016) 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 p.1657-1658
Abstract

Many microfluidic devices have been developed to separate rare cells, e.g. circulating tumor cells. The collection and analysis of the separated rare cells pose a challenge as it may lead to loss of target cells since extremely small number of cells are usually suspended in a large volume after separation. Here, we present an integrated device that allows acoustophoretic target cell separation and concentration, followed by dielectrophoretic single-cell trapping. We show that the human prostate cancer cell line DU145 were efficiently separated from peripheral blood mononuclear cells (PBMCs), and 81.7% of DU145 were trapped, with a contamination of 1.7% PBMC.

Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Acoustofluidics, Cell separation, CTC, Dielectrophoresis, Integrated device, Single-cell analysis
host publication
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
pages
2 pages
publisher
Chemical and Biological Microsystems Society
conference name
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
conference location
Dublin, Ireland
conference dates
2016-10-09 - 2016-10-13
external identifiers
  • scopus:85014169151
ISBN
9780979806490
language
English
LU publication?
yes
id
c1295ebc-f858-4dea-81f9-90243e785eb9
date added to LUP
2017-03-16 10:18:16
date last changed
2019-01-06 13:02:51
@inproceedings{c1295ebc-f858-4dea-81f9-90243e785eb9,
  abstract     = {<p>Many microfluidic devices have been developed to separate rare cells, e.g. circulating tumor cells. The collection and analysis of the separated rare cells pose a challenge as it may lead to loss of target cells since extremely small number of cells are usually suspended in a large volume after separation. Here, we present an integrated device that allows acoustophoretic target cell separation and concentration, followed by dielectrophoretic single-cell trapping. We show that the human prostate cancer cell line DU145 were efficiently separated from peripheral blood mononuclear cells (PBMCs), and 81.7% of DU145 were trapped, with a contamination of 1.7% PBMC.</p>},
  author       = {Antfolk, M. and Kim, S. H. and Saori, K. and Kaneda, Shohei and Fujii, T. and Laurell, T.},
  isbn         = {9780979806490},
  keyword      = {Acoustofluidics,Cell separation,CTC,Dielectrophoresis,Integrated device,Single-cell analysis},
  language     = {eng},
  location     = {Dublin, Ireland},
  pages        = {1657--1658},
  publisher    = {Chemical and Biological Microsystems Society},
  title        = {An integrated acousto- and dielectrophoresis device for tumor cell separation, concentration, and single-cell trapping},
  year         = {2016},
}