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An integrated acousto- and dielectrophoresis device for tumor cell separation, concentration, and single-cell trapping

Antfolk, M. LU ; Kim, S. H.; Saori, K.; Kaneda, Shohei; Fujii, T. and Laurell, T. LU (2016) 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 p.1657-1658
Abstract

Many microfluidic devices have been developed to separate rare cells, e.g. circulating tumor cells. The collection and analysis of the separated rare cells pose a challenge as it may lead to loss of target cells since extremely small number of cells are usually suspended in a large volume after separation. Here, we present an integrated device that allows acoustophoretic target cell separation and concentration, followed by dielectrophoretic single-cell trapping. We show that the human prostate cancer cell line DU145 were efficiently separated from peripheral blood mononuclear cells (PBMCs), and 81.7% of DU145 were trapped, with a contamination of 1.7% PBMC.

Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Acoustofluidics, Cell separation, CTC, Dielectrophoresis, Integrated device, Single-cell analysis
in
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
pages
2 pages
publisher
Chemical and Biological Microsystems Society
conference name
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
external identifiers
  • scopus:85014169151
ISBN
9780979806490
language
English
LU publication?
yes
id
c1295ebc-f858-4dea-81f9-90243e785eb9
date added to LUP
2017-03-16 10:18:16
date last changed
2017-03-20 09:48:09
@inproceedings{c1295ebc-f858-4dea-81f9-90243e785eb9,
  abstract     = {<p>Many microfluidic devices have been developed to separate rare cells, e.g. circulating tumor cells. The collection and analysis of the separated rare cells pose a challenge as it may lead to loss of target cells since extremely small number of cells are usually suspended in a large volume after separation. Here, we present an integrated device that allows acoustophoretic target cell separation and concentration, followed by dielectrophoretic single-cell trapping. We show that the human prostate cancer cell line DU145 were efficiently separated from peripheral blood mononuclear cells (PBMCs), and 81.7% of DU145 were trapped, with a contamination of 1.7% PBMC.</p>},
  author       = {Antfolk, M. and Kim, S. H. and Saori, K. and Kaneda, Shohei and Fujii, T. and Laurell, T.},
  booktitle    = {20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016},
  isbn         = {9780979806490},
  keyword      = {Acoustofluidics,Cell separation,CTC,Dielectrophoresis,Integrated device,Single-cell analysis},
  language     = {eng},
  pages        = {1657--1658},
  publisher    = {Chemical and Biological Microsystems Society},
  title        = {An integrated acousto- and dielectrophoresis device for tumor cell separation, concentration, and single-cell trapping},
  year         = {2016},
}