An integrated acousto- and dielectrophoresis device for tumor cell separation, concentration, and single-cell trapping
(2016) 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 p.1657-1658- Abstract
Many microfluidic devices have been developed to separate rare cells, e.g. circulating tumor cells. The collection and analysis of the separated rare cells pose a challenge as it may lead to loss of target cells since extremely small number of cells are usually suspended in a large volume after separation. Here, we present an integrated device that allows acoustophoretic target cell separation and concentration, followed by dielectrophoretic single-cell trapping. We show that the human prostate cancer cell line DU145 were efficiently separated from peripheral blood mononuclear cells (PBMCs), and 81.7% of DU145 were trapped, with a contamination of 1.7% PBMC.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/c1295ebc-f858-4dea-81f9-90243e785eb9
- author
- Antfolk, M. LU ; Kim, S. H. ; Saori, K. ; Kaneda, Shohei ; Fujii, T. and Laurell, T. LU
- organization
- publishing date
- 2016
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Acoustofluidics, Cell separation, CTC, Dielectrophoresis, Integrated device, Single-cell analysis
- host publication
- 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
- pages
- 2 pages
- publisher
- Chemical and Biological Microsystems Society
- conference name
- 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
- conference location
- Dublin, Ireland
- conference dates
- 2016-10-09 - 2016-10-13
- external identifiers
-
- scopus:85014169151
- ISBN
- 9780979806490
- language
- English
- LU publication?
- yes
- id
- c1295ebc-f858-4dea-81f9-90243e785eb9
- date added to LUP
- 2017-03-16 10:18:16
- date last changed
- 2023-10-05 01:45:02
@inproceedings{c1295ebc-f858-4dea-81f9-90243e785eb9, abstract = {{<p>Many microfluidic devices have been developed to separate rare cells, e.g. circulating tumor cells. The collection and analysis of the separated rare cells pose a challenge as it may lead to loss of target cells since extremely small number of cells are usually suspended in a large volume after separation. Here, we present an integrated device that allows acoustophoretic target cell separation and concentration, followed by dielectrophoretic single-cell trapping. We show that the human prostate cancer cell line DU145 were efficiently separated from peripheral blood mononuclear cells (PBMCs), and 81.7% of DU145 were trapped, with a contamination of 1.7% PBMC.</p>}}, author = {{Antfolk, M. and Kim, S. H. and Saori, K. and Kaneda, Shohei and Fujii, T. and Laurell, T.}}, booktitle = {{20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016}}, isbn = {{9780979806490}}, keywords = {{Acoustofluidics; Cell separation; CTC; Dielectrophoresis; Integrated device; Single-cell analysis}}, language = {{eng}}, pages = {{1657--1658}}, publisher = {{Chemical and Biological Microsystems Society}}, title = {{An integrated acousto- and dielectrophoresis device for tumor cell separation, concentration, and single-cell trapping}}, year = {{2016}}, }