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An integrated acousto- and dielectrophoresis device for tumor cell separation, concentration, and single-cell trapping

Antfolk, M. LU ; Kim, S. H. ; Saori, K. ; Kaneda, Shohei ; Fujii, T. and Laurell, T. LU (2016) 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 p.1657-1658
Abstract

Many microfluidic devices have been developed to separate rare cells, e.g. circulating tumor cells. The collection and analysis of the separated rare cells pose a challenge as it may lead to loss of target cells since extremely small number of cells are usually suspended in a large volume after separation. Here, we present an integrated device that allows acoustophoretic target cell separation and concentration, followed by dielectrophoretic single-cell trapping. We show that the human prostate cancer cell line DU145 were efficiently separated from peripheral blood mononuclear cells (PBMCs), and 81.7% of DU145 were trapped, with a contamination of 1.7% PBMC.

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author
; ; ; ; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Acoustofluidics, Cell separation, CTC, Dielectrophoresis, Integrated device, Single-cell analysis
host publication
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
pages
2 pages
publisher
Chemical and Biological Microsystems Society
conference name
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
conference location
Dublin, Ireland
conference dates
2016-10-09 - 2016-10-13
external identifiers
  • scopus:85014169151
ISBN
9780979806490
language
English
LU publication?
yes
id
c1295ebc-f858-4dea-81f9-90243e785eb9
date added to LUP
2017-03-16 10:18:16
date last changed
2023-10-05 01:45:02
@inproceedings{c1295ebc-f858-4dea-81f9-90243e785eb9,
  abstract     = {{<p>Many microfluidic devices have been developed to separate rare cells, e.g. circulating tumor cells. The collection and analysis of the separated rare cells pose a challenge as it may lead to loss of target cells since extremely small number of cells are usually suspended in a large volume after separation. Here, we present an integrated device that allows acoustophoretic target cell separation and concentration, followed by dielectrophoretic single-cell trapping. We show that the human prostate cancer cell line DU145 were efficiently separated from peripheral blood mononuclear cells (PBMCs), and 81.7% of DU145 were trapped, with a contamination of 1.7% PBMC.</p>}},
  author       = {{Antfolk, M. and Kim, S. H. and Saori, K. and Kaneda, Shohei and Fujii, T. and Laurell, T.}},
  booktitle    = {{20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016}},
  isbn         = {{9780979806490}},
  keywords     = {{Acoustofluidics; Cell separation; CTC; Dielectrophoresis; Integrated device; Single-cell analysis}},
  language     = {{eng}},
  pages        = {{1657--1658}},
  publisher    = {{Chemical and Biological Microsystems Society}},
  title        = {{An integrated acousto- and dielectrophoresis device for tumor cell separation, concentration, and single-cell trapping}},
  year         = {{2016}},
}