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GABPA inhibits invasion/metastasis in papillary thyroid carcinoma by regulating DICER1 expression

Yuan, Xiaotian; Mu, Ninni; Wang, Na; Strååt, Klas; Sofiadis, Anastasios; Guo, Yanxia; Stenman, Adam; Li, Kailin; Cheng, Guanghui and Zhang, Lu, et al. (2019) In Oncogene 38(7). p.965-979
Abstract

The ETS family transcription factor GABPA is suggested as an oncogenic element, which is further supported by the recent reporting of it as the sole ETS member to activate the mutant TERT promoter in thyroid carcinomas (TC). However, it remains unclear how GABPA contributes to TC pathogenesis. The present study is designed to address this issue. TERT expression was significantly diminished in TERT promoter-mutated TC cells upon GABPA inhibition. Surprisingly, GABPA depletion led to robustly increased cellular invasion independently of TERT promoter mutations and TERT expression. DICER1, a component of the microRNA machinery, was identified as a downstream effector of GABPA. GABPA facilitated Dicer1 transcription while its depletion... (More)

The ETS family transcription factor GABPA is suggested as an oncogenic element, which is further supported by the recent reporting of it as the sole ETS member to activate the mutant TERT promoter in thyroid carcinomas (TC). However, it remains unclear how GABPA contributes to TC pathogenesis. The present study is designed to address this issue. TERT expression was significantly diminished in TERT promoter-mutated TC cells upon GABPA inhibition. Surprisingly, GABPA depletion led to robustly increased cellular invasion independently of TERT promoter mutations and TERT expression. DICER1, a component of the microRNA machinery, was identified as a downstream effector of GABPA. GABPA facilitated Dicer1 transcription while its depletion reduced Dicer1 expression. The mutation of the GABPA binding site in the DICER1 promoter led to diminished basal levels of DICER1 promoter activity and abolishment of GABPA-stimulated promoter activity as well. The forced DICER1 expression abrogated the invasiveness of GABPA-depleted TC cells. Consistently, the analyses of 93 patients with papillary thyroid carcinoma (PTC) revealed a positive correlation between GABPA and DICER1 expression. GABPA expression was negatively associated with TERT expression and promoter mutations, in contrast to published observations in cancer cell lines. Lower GABPA expression was associated with distant metastasis and shorter overall/disease-free survival in PTC patients. Similar results were obtained for PTC cases in the TCGA dataset. In addition, a positive correlation between GABPA and DICER1 expression was seen in multiple types of malignancies. Taken together, despite its stimulatory effect on the mutant TERT promoter and telomerase activation, GABPA may itself act as a tumor suppressor rather than an oncogenic factor to inhibit invasion/metastasis in TCs and be a useful predictor for patient outcomes.

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Oncogene
volume
38
issue
7
pages
965 - 979
publisher
Nature Publishing Group
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  • scopus:85053402095
ISSN
0950-9232
DOI
10.1038/s41388-018-0483-x
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English
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yes
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c1367f33-fc68-4afd-8345-e291e98a7e00
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2018-10-24 11:56:26
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2019-08-14 04:25:53
@article{c1367f33-fc68-4afd-8345-e291e98a7e00,
  abstract     = {<p>The ETS family transcription factor GABPA is suggested as an oncogenic element, which is further supported by the recent reporting of it as the sole ETS member to activate the mutant TERT promoter in thyroid carcinomas (TC). However, it remains unclear how GABPA contributes to TC pathogenesis. The present study is designed to address this issue. TERT expression was significantly diminished in TERT promoter-mutated TC cells upon GABPA inhibition. Surprisingly, GABPA depletion led to robustly increased cellular invasion independently of TERT promoter mutations and TERT expression. DICER1, a component of the microRNA machinery, was identified as a downstream effector of GABPA. GABPA facilitated Dicer1 transcription while its depletion reduced Dicer1 expression. The mutation of the GABPA binding site in the DICER1 promoter led to diminished basal levels of DICER1 promoter activity and abolishment of GABPA-stimulated promoter activity as well. The forced DICER1 expression abrogated the invasiveness of GABPA-depleted TC cells. Consistently, the analyses of 93 patients with papillary thyroid carcinoma (PTC) revealed a positive correlation between GABPA and DICER1 expression. GABPA expression was negatively associated with TERT expression and promoter mutations, in contrast to published observations in cancer cell lines. Lower GABPA expression was associated with distant metastasis and shorter overall/disease-free survival in PTC patients. Similar results were obtained for PTC cases in the TCGA dataset. In addition, a positive correlation between GABPA and DICER1 expression was seen in multiple types of malignancies. Taken together, despite its stimulatory effect on the mutant TERT promoter and telomerase activation, GABPA may itself act as a tumor suppressor rather than an oncogenic factor to inhibit invasion/metastasis in TCs and be a useful predictor for patient outcomes.</p>},
  author       = {Yuan, Xiaotian and Mu, Ninni and Wang, Na and Strååt, Klas and Sofiadis, Anastasios and Guo, Yanxia and Stenman, Adam and Li, Kailin and Cheng, Guanghui and Zhang, Lu and Kong, Feng and Ekblad, Lars and Wennerberg, Johan and Nilsson, Inga Lena and Juhlin, C. Christofer and Larsson, Catharina and Xu, Dawei},
  issn         = {0950-9232},
  language     = {eng},
  number       = {7},
  pages        = {965--979},
  publisher    = {Nature Publishing Group},
  series       = {Oncogene},
  title        = {GABPA inhibits invasion/metastasis in papillary thyroid carcinoma by regulating DICER1 expression},
  url          = {http://dx.doi.org/10.1038/s41388-018-0483-x},
  volume       = {38},
  year         = {2019},
}