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The predominant protein in human seminal coagulate

Lilja, H. LU orcid and Laurell, C. B. LU (1985) In Scandinavian Journal of Clinical and Laboratory Investigation 45(7). p.635-641
Abstract

The predominant protein in human seminal vesicle secretion constitutes the structural protein of coagulated semen. This high molecular weight protein (HMW-SV-protein) is stable in seminal vesicle secretion during in vitro storage at 37 d̀C for at least 20 h, but is rapidly cleaved on mixing with prostatic proteases. Seminal coagulate, washed free of souble components, is dissoluble by 2 to 3 mol/1 of guanidine-HCl. Although dithiothreitol added to seminal coagulate does not liquify the clot, complexes between HMW-SV-proteins are broken up by reduction under denaturing conditions, which suggests that the non-covalent linkages of HMW-SV-proteins are essential in the clot. Prostatic proteases cleave the HMW-SV-protein during liquefaction... (More)

The predominant protein in human seminal vesicle secretion constitutes the structural protein of coagulated semen. This high molecular weight protein (HMW-SV-protein) is stable in seminal vesicle secretion during in vitro storage at 37 d̀C for at least 20 h, but is rapidly cleaved on mixing with prostatic proteases. Seminal coagulate, washed free of souble components, is dissoluble by 2 to 3 mol/1 of guanidine-HCl. Although dithiothreitol added to seminal coagulate does not liquify the clot, complexes between HMW-SV-proteins are broken up by reduction under denaturing conditions, which suggests that the non-covalent linkages of HMW-SV-proteins are essential in the clot. Prostatic proteases cleave the HMW-SV-protein during liquefaction of ejaculated semen to a series of labile proteins. These proteins are further cleaved to peptides of successively decreasing size after completed liquefaction. The cleavage of the HMW-SV-protein is the major cause of the fast shift of the electrophoretic pattern of seminal proteins if semen is stored without protease inhibitors.

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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Inhibin, Prostate, Protease inhibitors, Proteolytic enzymes, Semen
in
Scandinavian Journal of Clinical and Laboratory Investigation
volume
45
issue
7
pages
635 - 641
publisher
Informa Healthcare
external identifiers
  • scopus:0022239505
  • pmid:2866578
ISSN
0036-5513
DOI
10.3109/00365518509155271
language
English
LU publication?
yes
id
c138c476-a2a7-428c-b8ea-f85e564d05cc
date added to LUP
2022-12-06 16:53:07
date last changed
2024-01-18 15:55:46
@article{c138c476-a2a7-428c-b8ea-f85e564d05cc,
  abstract     = {{<p>The predominant protein in human seminal vesicle secretion constitutes the structural protein of coagulated semen. This high molecular weight protein (HMW-SV-protein) is stable in seminal vesicle secretion during in vitro storage at 37 d̀C for at least 20 h, but is rapidly cleaved on mixing with prostatic proteases. Seminal coagulate, washed free of souble components, is dissoluble by 2 to 3 mol/1 of guanidine-HCl. Although dithiothreitol added to seminal coagulate does not liquify the clot, complexes between HMW-SV-proteins are broken up by reduction under denaturing conditions, which suggests that the non-covalent linkages of HMW-SV-proteins are essential in the clot. Prostatic proteases cleave the HMW-SV-protein during liquefaction of ejaculated semen to a series of labile proteins. These proteins are further cleaved to peptides of successively decreasing size after completed liquefaction. The cleavage of the HMW-SV-protein is the major cause of the fast shift of the electrophoretic pattern of seminal proteins if semen is stored without protease inhibitors.</p>}},
  author       = {{Lilja, H. and Laurell, C. B.}},
  issn         = {{0036-5513}},
  keywords     = {{Inhibin; Prostate; Protease inhibitors; Proteolytic enzymes; Semen}},
  language     = {{eng}},
  number       = {{7}},
  pages        = {{635--641}},
  publisher    = {{Informa Healthcare}},
  series       = {{Scandinavian Journal of Clinical and Laboratory Investigation}},
  title        = {{The predominant protein in human seminal coagulate}},
  url          = {{http://dx.doi.org/10.3109/00365518509155271}},
  doi          = {{10.3109/00365518509155271}},
  volume       = {{45}},
  year         = {{1985}},
}