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Regulation of bone sialoprotein mRNA by steroid hormones.

Oldberg, Åke LU ; Jirskog-Hed, B ; Axelsson, Sara LU and Heinegård, Dick LU (1989) In Journal of Cell Biology 109(6 Pt 1). p.3183-3186
Abstract
In this report we demonstrate an increase in the steady-state level of bone sialoprotein (BSP) mRNA in rat calvaria and a rat osteosarcoma cell line (ROS 17/2.8) after treatment with the synthetic glucocorticoid, dexamethasone. In contrast, 1.25-dihydroxyvitamin D3 reduced the amount of BSP mRNA in calvaria and inhibited the dexamethasone induction in ROS 17/2.8 cells. The increase in BSP mRNA is most likely due to an increase in the transcriptional rate. The stability of mRNA was unchanged after dexamethasone treatment with a half-life of approximately 5 h. Nuclear transcription experiments with nuclei isolated from ROS 17/2.8 cells showed an increased BSP mRNA synthesis in cells treated with dexamethasone.
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author
; ; and
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type
Contribution to journal
publication status
published
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keywords
Messenger: genetics, Messenger: drug effects, RNA, Genes: drug effects, Gene Expression Regulation: drug effects, Dexamethasone: pharmacology, Calcitriol: pharmacology, Bone and Bones: drug effects, Bone and Bones: metabolism, Sialoglycoproteins: genetics, Transcription, Genetic: drug effects
in
Journal of Cell Biology
volume
109
issue
6 Pt 1
pages
3183 - 3186
publisher
Rockefeller University Press
external identifiers
  • pmid:2592421
  • scopus:0024850620
ISSN
0021-9525
language
English
LU publication?
yes
id
c180189c-47e0-450a-9edf-f51b971f2acc (old id 3632721)
alternative location
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2115918/
date added to LUP
2016-04-01 11:44:17
date last changed
2021-09-26 05:09:30
@article{c180189c-47e0-450a-9edf-f51b971f2acc,
  abstract     = {{In this report we demonstrate an increase in the steady-state level of bone sialoprotein (BSP) mRNA in rat calvaria and a rat osteosarcoma cell line (ROS 17/2.8) after treatment with the synthetic glucocorticoid, dexamethasone. In contrast, 1.25-dihydroxyvitamin D3 reduced the amount of BSP mRNA in calvaria and inhibited the dexamethasone induction in ROS 17/2.8 cells. The increase in BSP mRNA is most likely due to an increase in the transcriptional rate. The stability of mRNA was unchanged after dexamethasone treatment with a half-life of approximately 5 h. Nuclear transcription experiments with nuclei isolated from ROS 17/2.8 cells showed an increased BSP mRNA synthesis in cells treated with dexamethasone.}},
  author       = {{Oldberg, Åke and Jirskog-Hed, B and Axelsson, Sara and Heinegård, Dick}},
  issn         = {{0021-9525}},
  keywords     = {{Messenger: genetics; Messenger: drug effects; RNA; Genes: drug effects; Gene Expression Regulation: drug effects; Dexamethasone: pharmacology; Calcitriol: pharmacology; Bone and Bones: drug effects; Bone and Bones: metabolism; Sialoglycoproteins: genetics; Transcription; Genetic: drug effects}},
  language     = {{eng}},
  number       = {{6 Pt 1}},
  pages        = {{3183--3186}},
  publisher    = {{Rockefeller University Press}},
  series       = {{Journal of Cell Biology}},
  title        = {{Regulation of bone sialoprotein mRNA by steroid hormones.}},
  url          = {{http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2115918/}},
  volume       = {{109}},
  year         = {{1989}},
}