Heparan sulfate in perlecan promotes mouse atherosclerosis : Roles in lipid permeability, lipid retention, and smooth muscle cell proliferation
(2008) In Circulation Research 103(1). p.43-52- Abstract
Heparan sulfate (HS) has been proposed to be antiatherogenic through inhibition of lipoprotein retention, inflammation, and smooth muscle cell proliferation. Perlecan is the predominant HS proteoglycan in the artery wall. Here, we investigated the role of perlecan HS chains using apoE null (ApoE0) mice that were cross-bred with mice expressing HS-deficient perlecan (Hspg2 Δ3/Δ3). Morphometry of cross-sections from aortic roots and en face preparations of whole aortas revealed a significant decrease in lesion formation in ApoE0/Hspg2 Δ3/Δ3mice at both 15 and 33 weeks. In vitro, binding of labeled mouse triglyceride-rich lipoproteins and human LDL to total extracellular matrix, as well as to purified proteoglycans,... (More)
Heparan sulfate (HS) has been proposed to be antiatherogenic through inhibition of lipoprotein retention, inflammation, and smooth muscle cell proliferation. Perlecan is the predominant HS proteoglycan in the artery wall. Here, we investigated the role of perlecan HS chains using apoE null (ApoE0) mice that were cross-bred with mice expressing HS-deficient perlecan (Hspg2 Δ3/Δ3). Morphometry of cross-sections from aortic roots and en face preparations of whole aortas revealed a significant decrease in lesion formation in ApoE0/Hspg2 Δ3/Δ3mice at both 15 and 33 weeks. In vitro, binding of labeled mouse triglyceride-rich lipoproteins and human LDL to total extracellular matrix, as well as to purified proteoglycans, prepared from ApoE0/Hspg2 Δ3/Δ3 smooth muscle cells was reduced. In vivo, at 20 minutes influx of human 125I-LDL or mouse triglyceride-rich lipoproteins into the aortic wall was increased in ApoE0/Hspg2 Δ3/Δ3 mice compared to ApoE0 mice. However, at 72 hours accumulation of 125I-LDL was similar in ApoE0/Hspg2 Δ3/Δ3and ApoEO mice. Immunohistochemistry of lesions from ApoE0/Hspg2 Δ3/Δ3 mice showed decreased staining for apoB and increased smooth muscle α-actin content, whereas accumulation of CD68-positive inflammatory cells was unchanged. We conclude that the perlecan HS chains are proatherogenic in mice, possibly through increased lipoprotein retention, altered vascular permeability, or other mechanisms. The ability of HS to inhibit smooth muscle cell growth may also influence development as well as instability of lesions.
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- author
- Tran-Lundmark, Karin LU ; Tran, Phan Kiet LU ; Paulsson-Berne, Gabrielle ; Fridén, Vincent ; Soininen, Raija ; Tryggvason, Karl ; Wight, Thomas N. ; Kinsella, Michael G. ; Borén, Jan and Hedin, Ulf
- publishing date
- 2008-07-03
- type
- Contribution to journal
- publication status
- published
- keywords
- Atherosclerosis, Heparan sulfate, Lipoproteins, Perlecan, Smooth muscle cells
- in
- Circulation Research
- volume
- 103
- issue
- 1
- pages
- 10 pages
- publisher
- American Heart Association
- external identifiers
-
- scopus:48049100574
- pmid:18596265
- ISSN
- 0009-7330
- DOI
- 10.1161/CIRCRESAHA.107.172833
- language
- English
- LU publication?
- no
- id
- c1f3c533-7c74-447b-a548-453355fd1dc0
- date added to LUP
- 2019-07-01 22:16:42
- date last changed
- 2024-06-12 23:41:18
@article{c1f3c533-7c74-447b-a548-453355fd1dc0,
abstract = {{<p>Heparan sulfate (HS) has been proposed to be antiatherogenic through inhibition of lipoprotein retention, inflammation, and smooth muscle cell proliferation. Perlecan is the predominant HS proteoglycan in the artery wall. Here, we investigated the role of perlecan HS chains using apoE null (ApoE0) mice that were cross-bred with mice expressing HS-deficient perlecan (Hspg2 <sup>Δ3/Δ3</sup>). Morphometry of cross-sections from aortic roots and en face preparations of whole aortas revealed a significant decrease in lesion formation in ApoE0/Hspg2 <sup>Δ3/Δ3</sup>mice at both 15 and 33 weeks. In vitro, binding of labeled mouse triglyceride-rich lipoproteins and human LDL to total extracellular matrix, as well as to purified proteoglycans, prepared from ApoE0/Hspg2 <sup>Δ3/Δ3</sup> smooth muscle cells was reduced. In vivo, at 20 minutes influx of human <sup>125</sup>I-LDL or mouse triglyceride-rich lipoproteins into the aortic wall was increased in ApoE0/Hspg2 <sup>Δ3/Δ3</sup> mice compared to ApoE0 mice. However, at 72 hours accumulation of <sup>125</sup>I-LDL was similar in ApoE0/Hspg2 <sup>Δ3/Δ3</sup>and ApoEO mice. Immunohistochemistry of lesions from ApoE0/Hspg2 <sup>Δ3/Δ3</sup> mice showed decreased staining for apoB and increased smooth muscle α-actin content, whereas accumulation of CD68-positive inflammatory cells was unchanged. We conclude that the perlecan HS chains are proatherogenic in mice, possibly through increased lipoprotein retention, altered vascular permeability, or other mechanisms. The ability of HS to inhibit smooth muscle cell growth may also influence development as well as instability of lesions.</p>}},
author = {{Tran-Lundmark, Karin and Tran, Phan Kiet and Paulsson-Berne, Gabrielle and Fridén, Vincent and Soininen, Raija and Tryggvason, Karl and Wight, Thomas N. and Kinsella, Michael G. and Borén, Jan and Hedin, Ulf}},
issn = {{0009-7330}},
keywords = {{Atherosclerosis; Heparan sulfate; Lipoproteins; Perlecan; Smooth muscle cells}},
language = {{eng}},
month = {{07}},
number = {{1}},
pages = {{43--52}},
publisher = {{American Heart Association}},
series = {{Circulation Research}},
title = {{Heparan sulfate in perlecan promotes mouse atherosclerosis : Roles in lipid permeability, lipid retention, and smooth muscle cell proliferation}},
url = {{http://dx.doi.org/10.1161/CIRCRESAHA.107.172833}},
doi = {{10.1161/CIRCRESAHA.107.172833}},
volume = {{103}},
year = {{2008}},
}