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Injury-induced hsp27 expression in peripheral nervous tissue is not associated with any alteration in axonal outgrowth after immediate or delayed nerve repair

Stenberg, Lena LU orcid ; Hazer Rosberg, Derya Burcu LU orcid ; Kohyama, Sho LU ; Suganuma, Seigo LU and Dahlin, Lars B. LU orcid (2021) In International Journal of Molecular Sciences 22(16).
Abstract

We investigated injury-induced heat shock protein 27 (HSP27) expression and its association to axonal outgrowth after injury and different nerve repair models in healthy Wistar and diabetic Goto-Kakizaki rats. By immunohistochemistry, expression of HSP27 in sciatic nerves and DRG and axonal outgrowth (neurofilaments) in sciatic nerves were analyzed after no, immediate, and delayed (7-day delay) nerve repairs (7-or 14-day follow-up). An increased HSP27 expression in nerves and in DRG at the uninjured side was associated with diabetes. HSP27 expression in nerves and in DRG increased substantially after the nerve injuries, being higher at the site where axons and Schwann cells interacted. Regression analysis indicated a positive influence... (More)

We investigated injury-induced heat shock protein 27 (HSP27) expression and its association to axonal outgrowth after injury and different nerve repair models in healthy Wistar and diabetic Goto-Kakizaki rats. By immunohistochemistry, expression of HSP27 in sciatic nerves and DRG and axonal outgrowth (neurofilaments) in sciatic nerves were analyzed after no, immediate, and delayed (7-day delay) nerve repairs (7-or 14-day follow-up). An increased HSP27 expression in nerves and in DRG at the uninjured side was associated with diabetes. HSP27 expression in nerves and in DRG increased substantially after the nerve injuries, being higher at the site where axons and Schwann cells interacted. Regression analysis indicated a positive influence of immediate nerve repair compared to an unrepaired injury, but a shortly delayed nerve repair had no impact on axonal outgrowth. Diabetes was associated with a decreased axonal outgrowth. The increased expression of HSP27 in sciatic nerve and DRG did not influence axonal outgrowth. Injured sciatic nerves should appropriately be repaired in healthy and diabetic rats, but a short delay does not influence axonal outgrowth. HSP27 expression in sciatic nerve or DRG, despite an increase after nerve injury with or without a repair, is not associated with any alteration in axonal outgrowth.

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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Diabetes, DRG, HSP27, Nerve injury, Nerve repair
in
International Journal of Molecular Sciences
volume
22
issue
16
article number
8624
publisher
MDPI AG
external identifiers
  • pmid:34445330
  • scopus:85112365782
ISSN
1661-6596
DOI
10.3390/ijms22168624
project
Nerve regeneration in healthy and diabetic rat sciatic nerve - influence by timing, a novel scaffold and the synthetic peptide PLX01
language
English
LU publication?
yes
id
c2d7a6dd-6112-440f-b299-85f6a6dcd69b
date added to LUP
2021-09-20 15:13:13
date last changed
2024-10-20 06:09:46
@article{c2d7a6dd-6112-440f-b299-85f6a6dcd69b,
  abstract     = {{<p>We investigated injury-induced heat shock protein 27 (HSP27) expression and its association to axonal outgrowth after injury and different nerve repair models in healthy Wistar and diabetic Goto-Kakizaki rats. By immunohistochemistry, expression of HSP27 in sciatic nerves and DRG and axonal outgrowth (neurofilaments) in sciatic nerves were analyzed after no, immediate, and delayed (7-day delay) nerve repairs (7-or 14-day follow-up). An increased HSP27 expression in nerves and in DRG at the uninjured side was associated with diabetes. HSP27 expression in nerves and in DRG increased substantially after the nerve injuries, being higher at the site where axons and Schwann cells interacted. Regression analysis indicated a positive influence of immediate nerve repair compared to an unrepaired injury, but a shortly delayed nerve repair had no impact on axonal outgrowth. Diabetes was associated with a decreased axonal outgrowth. The increased expression of HSP27 in sciatic nerve and DRG did not influence axonal outgrowth. Injured sciatic nerves should appropriately be repaired in healthy and diabetic rats, but a short delay does not influence axonal outgrowth. HSP27 expression in sciatic nerve or DRG, despite an increase after nerve injury with or without a repair, is not associated with any alteration in axonal outgrowth.</p>}},
  author       = {{Stenberg, Lena and Hazer Rosberg, Derya Burcu and Kohyama, Sho and Suganuma, Seigo and Dahlin, Lars B.}},
  issn         = {{1661-6596}},
  keywords     = {{Diabetes; DRG; HSP27; Nerve injury; Nerve repair}},
  language     = {{eng}},
  month        = {{08}},
  number       = {{16}},
  publisher    = {{MDPI AG}},
  series       = {{International Journal of Molecular Sciences}},
  title        = {{Injury-induced hsp27 expression in peripheral nervous tissue is not associated with any alteration in axonal outgrowth after immediate or delayed nerve repair}},
  url          = {{http://dx.doi.org/10.3390/ijms22168624}},
  doi          = {{10.3390/ijms22168624}},
  volume       = {{22}},
  year         = {{2021}},
}