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Molecular and functional definition of the developing human striatum

Onorati, Marco; Castiglioni, Valentina; Biasci, Daniele; Cesana, Elisabetta; Menon, Ramesh; Vuono, Romina; Talpo, Francesca; Laguna Goya, Rocio; Lyons, Paul A and Bulfamante, Gaetano P, et al. (2014) In Nature Neuroscience 17(12). p.15-1804
Abstract

The complexity of the human brain derives from the intricate interplay of molecular instructions during development. Here we systematically investigated gene expression changes in the prenatal human striatum and cerebral cortex during development from post-conception weeks 2 to 20. We identified tissue-specific gene coexpression networks, differentially expressed genes and a minimal set of bimodal genes, including those encoding transcription factors, that distinguished striatal from neocortical identities. Unexpected differences from mouse striatal development were discovered. We monitored 36 determinants at the protein level, revealing regional domains of expression and their refinement, during striatal development. We... (More)

The complexity of the human brain derives from the intricate interplay of molecular instructions during development. Here we systematically investigated gene expression changes in the prenatal human striatum and cerebral cortex during development from post-conception weeks 2 to 20. We identified tissue-specific gene coexpression networks, differentially expressed genes and a minimal set of bimodal genes, including those encoding transcription factors, that distinguished striatal from neocortical identities. Unexpected differences from mouse striatal development were discovered. We monitored 36 determinants at the protein level, revealing regional domains of expression and their refinement, during striatal development. We electrophysiologically profiled human striatal neurons differentiated in vitro and determined their refined molecular and functional properties. These results provide a resource and opportunity to gain global understanding of how transcriptional and functional processes converge to specify human striatal and neocortical neurons during development.

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keywords
Action Potentials, Cell Differentiation, Cells, Cultured, Corpus Striatum, Fetal Development, Gene Regulatory Networks, HEK293 Cells, Humans, Organ Culture Techniques, Journal Article, Research Support, Non-U.S. Gov't
in
Nature Neuroscience
volume
17
issue
12
pages
12 pages
publisher
Nature Publishing Group
external identifiers
  • scopus:84926162823
ISSN
1546-1726
DOI
10.1038/nn.3860
language
English
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no
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c318e465-9c70-4dcd-b65d-79553a416117
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2016-11-24 15:01:35
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2017-11-19 04:35:10
@article{c318e465-9c70-4dcd-b65d-79553a416117,
  abstract     = {<p>The complexity of the human brain derives from the intricate interplay of molecular instructions during development. Here we systematically investigated gene expression changes in the prenatal human striatum and cerebral cortex during development from post-conception weeks 2 to 20. We identified tissue-specific gene coexpression networks, differentially expressed genes and a minimal set of bimodal genes, including those encoding transcription factors, that distinguished striatal from neocortical identities. Unexpected differences from mouse striatal development were discovered. We monitored 36 determinants at the protein level, revealing regional domains of expression and their refinement, during striatal development. We electrophysiologically profiled human striatal neurons differentiated in vitro and determined their refined molecular and functional properties. These results provide a resource and opportunity to gain global understanding of how transcriptional and functional processes converge to specify human striatal and neocortical neurons during development.</p>},
  author       = {Onorati, Marco and Castiglioni, Valentina and Biasci, Daniele and Cesana, Elisabetta and Menon, Ramesh and Vuono, Romina and Talpo, Francesca and Laguna Goya, Rocio and Lyons, Paul A and Bulfamante, Gaetano P and Muzio, Luca and Martino, Gianvito and Toselli, Mauro and Farina, Cinthia and Barker, Roger A and Biella, Gerardo and Cattaneo, Elena},
  issn         = {1546-1726},
  keyword      = {Action Potentials,Cell Differentiation,Cells, Cultured,Corpus Striatum,Fetal Development,Gene Regulatory Networks,HEK293 Cells,Humans,Organ Culture Techniques,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {12},
  pages        = {15--1804},
  publisher    = {Nature Publishing Group},
  series       = {Nature Neuroscience},
  title        = {Molecular and functional definition of the developing human striatum},
  url          = {http://dx.doi.org/10.1038/nn.3860},
  volume       = {17},
  year         = {2014},
}