Sound to guide cells
(2024) Medicinteknikdagarna 2024- Abstract
- By combining acoustic fields and liquid flows in microchannels we develop tools to chart and separate
biological particles based on their bio-mechanical properties. I will present an overview of three ongoing
projects where acoustic forces are utilized to separate blood cells.
We studied the packing behavior of whole blood in a sound field. We discovered that cells self-organize
such that red blood cells occupy a region nearest the acoustic pressure node while cancer cells migrate
to the plasma interface. This can be done in a flow-through device and more than 90% of red blood cells
can be removed in continuous flow while recovering >50% of the target cells. [1]
In a second project, we developed a method to... (More) - By combining acoustic fields and liquid flows in microchannels we develop tools to chart and separate
biological particles based on their bio-mechanical properties. I will present an overview of three ongoing
projects where acoustic forces are utilized to separate blood cells.
We studied the packing behavior of whole blood in a sound field. We discovered that cells self-organize
such that red blood cells occupy a region nearest the acoustic pressure node while cancer cells migrate
to the plasma interface. This can be done in a flow-through device and more than 90% of red blood cells
can be removed in continuous flow while recovering >50% of the target cells. [1]
In a second project, we developed a method to separate and measure the acoustic properties of
thousands of single cells by tracking their location in a media that forms a gradient in acoustic properties.
The cells migrate due to the sound field until reaching their point of zero acoustic contrast. From single-
cell properties, we can predict the behavior of cells in any medium and acoustic field and design and
optimize separation schemes. [2]
In another project, we showed that we can enrich clusters of circulating tumor cells from white blood
cells by acoustic separation, Fig 1. We analyzed blood from 12 patients with advanced-stage prostate
cancer and compared that to blood from 20 healthy donors. We found that circulating tumor cell clusters
in most patients but in very few of the control subjects. [3]
Our vision is that we will be able to integrate the different unit operations for cell separation that we have
demonstrated into one process flow, thus building a system that can take whole blood as input and
highly refined sub-populations of cells as output.
References
[1] Soller, in https://acoustofluidics.net/archive/materials/Acoustofluidics_2023_Materials.pdf (2023).
[2] https://doi.org/10.1038/ncomms11556 (2016).
[3] https://doi.org/10.1021/acs.analchem.3c05371 (2024). (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/c399f48a-52d6-4dfd-b747-5e78292b87c3
- author
- Augustsson, Per LU
- organization
- publishing date
- 2024-10-09
- type
- Contribution to conference
- publication status
- published
- subject
- conference name
- Medicinteknikdagarna 2024
- conference location
- Göteborg, Sweden
- conference dates
- 2024-10-09 - 2024-10-10
- project
- Akustisk flödescytometri för cancerdiagnostik
- High Throughput Microfluidic Cell Nanoparticle Handling by Molecular and Thermal Gradient Acoustic Focusing
- language
- English
- LU publication?
- yes
- id
- c399f48a-52d6-4dfd-b747-5e78292b87c3
- date added to LUP
- 2024-12-02 14:24:29
- date last changed
- 2025-04-04 14:15:51
@misc{c399f48a-52d6-4dfd-b747-5e78292b87c3,
abstract = {{By combining acoustic fields and liquid flows in microchannels we develop tools to chart and separate<br/>biological particles based on their bio-mechanical properties. I will present an overview of three ongoing<br/>projects where acoustic forces are utilized to separate blood cells.<br/>We studied the packing behavior of whole blood in a sound field. We discovered that cells self-organize<br/>such that red blood cells occupy a region nearest the acoustic pressure node while cancer cells migrate<br/>to the plasma interface. This can be done in a flow-through device and more than 90% of red blood cells<br/>can be removed in continuous flow while recovering >50% of the target cells. [1]<br/>In a second project, we developed a method to separate and measure the acoustic properties of<br/>thousands of single cells by tracking their location in a media that forms a gradient in acoustic properties.<br/>The cells migrate due to the sound field until reaching their point of zero acoustic contrast. From single-<br/>cell properties, we can predict the behavior of cells in any medium and acoustic field and design and<br/>optimize separation schemes. [2]<br/>In another project, we showed that we can enrich clusters of circulating tumor cells from white blood<br/>cells by acoustic separation, Fig 1. We analyzed blood from 12 patients with advanced-stage prostate<br/>cancer and compared that to blood from 20 healthy donors. We found that circulating tumor cell clusters<br/>in most patients but in very few of the control subjects. [3]<br/>Our vision is that we will be able to integrate the different unit operations for cell separation that we have<br/>demonstrated into one process flow, thus building a system that can take whole blood as input and<br/>highly refined sub-populations of cells as output. <br/>References<br/>[1] Soller, in https://acoustofluidics.net/archive/materials/Acoustofluidics_2023_Materials.pdf (2023).<br/>[2] https://doi.org/10.1038/ncomms11556 (2016).<br/>[3] https://doi.org/10.1021/acs.analchem.3c05371 (2024).}},
author = {{Augustsson, Per}},
language = {{eng}},
month = {{10}},
title = {{Sound to guide cells}},
year = {{2024}},
}