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Ten-color 15-antibody flow cytometry panel for immunophenotyping of lymphocyte population

Rajab, Amr; Axler, Olof LU ; Leung, Jessie; Wozniak, Malgorzata and Porwit, Anna LU (2017) In International Journal of Laboratory Hematology 39. p.76-85
Abstract

We have developed a lymphoproliferative disorder screening tube (LPD-ST) with the aim to provide comprehensive immunophenotyping of lymphocyte subsets with minimal need for additional testing. The LPD-ST consists of CD4/kappa FITC, CD8/lambda PE, CD3/CD14ECD, CD38PC5.5, CD20/CD56PC7, CD10APC, CD19APC-A700, CD5APC-A750, CD57/CD23PB and CD45KO. The LPD-ST was validated against previously used lymphocyte subset panels in Canada (n=60) and in Sweden (n=43) and against the OneFlow LST (n=60). The LPD-ST panel was then implemented in clinical practice using dried monoclonal antibody reagents (Duraclone®) on 649 patient samples in Sweden. In 204 of 649 samples (31%), a monotypic B-cell population was found. Of these... (More)

We have developed a lymphoproliferative disorder screening tube (LPD-ST) with the aim to provide comprehensive immunophenotyping of lymphocyte subsets with minimal need for additional testing. The LPD-ST consists of CD4/kappa FITC, CD8/lambda PE, CD3/CD14ECD, CD38PC5.5, CD20/CD56PC7, CD10APC, CD19APC-A700, CD5APC-A750, CD57/CD23PB and CD45KO. The LPD-ST was validated against previously used lymphocyte subset panels in Canada (n=60) and in Sweden (n=43) and against the OneFlow LST (n=60). The LPD-ST panel was then implemented in clinical practice using dried monoclonal antibody reagents (Duraclone®) on 649 patient samples in Sweden. In 204 of 649 samples (31%), a monotypic B-cell population was found. Of these cases, a final diagnosis could be rendered in 106 cases (52%), and in the remainder, additional B-cell immunophenotyping was performed. In 20 (3%) samples, an aberrant T-cell population was confirmed by additional testing. Of 425 samples diagnosed as normal/reactive lymphoid tissue, 50 (12%) required additional immunophenotyping, mostly due to an abnormal CD4/CD8 ratio. The LPD-ST tube significantly minimizes the need for additional testing, improves the turn-around time, and reduces the cost of LPD immunophenotyping. It is also suitable for investigating paucicellular samples such as cerebrospinal fluid or fine needle aspirates.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
lymphocyte, lymphoma, multiparameter flow cytometry, screening
in
International Journal of Laboratory Hematology
volume
39
pages
10 pages
publisher
Wiley-Blackwell
external identifiers
  • scopus:85018677412
  • wos:000400189400013
ISSN
1751-5521
DOI
10.1111/ijlh.12678
language
English
LU publication?
yes
id
c46f6624-e00b-4d5a-bdc8-103248bfbb33
date added to LUP
2017-06-08 14:49:45
date last changed
2017-09-18 11:39:44
@article{c46f6624-e00b-4d5a-bdc8-103248bfbb33,
  abstract     = {<p>We have developed a lymphoproliferative disorder screening tube (LPD-ST) with the aim to provide comprehensive immunophenotyping of lymphocyte subsets with minimal need for additional testing. The LPD-ST consists of CD4/kappa FITC, CD8/lambda PE, CD3/CD14ECD, CD38PC5.5, CD20/CD56PC7, CD10APC, CD19APC-A700, CD5APC-A750, CD57/CD23PB and CD45KO. The LPD-ST was validated against previously used lymphocyte subset panels in Canada (n=60) and in Sweden (n=43) and against the OneFlow<sup>™</sup> LST (n=60). The LPD-ST panel was then implemented in clinical practice using dried monoclonal antibody reagents (Duraclone<sup>®</sup>) on 649 patient samples in Sweden. In 204 of 649 samples (31%), a monotypic B-cell population was found. Of these cases, a final diagnosis could be rendered in 106 cases (52%), and in the remainder, additional B-cell immunophenotyping was performed. In 20 (3%) samples, an aberrant T-cell population was confirmed by additional testing. Of 425 samples diagnosed as normal/reactive lymphoid tissue, 50 (12%) required additional immunophenotyping, mostly due to an abnormal CD4/CD8 ratio. The LPD-ST tube significantly minimizes the need for additional testing, improves the turn-around time, and reduces the cost of LPD immunophenotyping. It is also suitable for investigating paucicellular samples such as cerebrospinal fluid or fine needle aspirates.</p>},
  author       = {Rajab, Amr and Axler, Olof and Leung, Jessie and Wozniak, Malgorzata and Porwit, Anna},
  issn         = {1751-5521},
  keyword      = {lymphocyte,lymphoma,multiparameter flow cytometry,screening},
  language     = {eng},
  month        = {05},
  pages        = {76--85},
  publisher    = {Wiley-Blackwell},
  series       = {International Journal of Laboratory Hematology},
  title        = {Ten-color 15-antibody flow cytometry panel for immunophenotyping of lymphocyte population},
  url          = {http://dx.doi.org/10.1111/ijlh.12678},
  volume       = {39},
  year         = {2017},
}