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Structural basis for activation of plasma-membrane Ca2+-ATPase by calmodulin

Nitsche, Julius ; Josts, Inokentijs ; Heidemann, Johannes ; Mertens, Haydyn D ; Maric, Selma LU ; Moulin, Martine ; Haertlein, Michael ; Busch, Sebastian ; Forsyth, V Trevor and Svergun, Dmitri I , et al. (2018) In Communications Biology 1. p.206-206
Abstract

Plasma-membrane Ca2+-ATPases expel Ca2+ from the cytoplasm and are key regulators of Ca2+ homeostasis in eukaryotes. They are autoinhibited under low Ca2+ concentrations. Calmodulin (CaM)-binding to a unique regulatory domain releases the autoinhibition and activates the pump. However, the structural basis for this activation, including the overall structure of this calcium pump and its complex with calmodulin, is unknown. We previously determined the high-resolution structure of calmodulin in complex with the regulatory domain of the plasma-membrane Ca2+-ATPase ACA8 and revealed a bimodular mechanism of calcium control in eukaryotes. Here we show that activation of ACA8 by CaM involves large conformational changes. Combining advanced... (More)

Plasma-membrane Ca2+-ATPases expel Ca2+ from the cytoplasm and are key regulators of Ca2+ homeostasis in eukaryotes. They are autoinhibited under low Ca2+ concentrations. Calmodulin (CaM)-binding to a unique regulatory domain releases the autoinhibition and activates the pump. However, the structural basis for this activation, including the overall structure of this calcium pump and its complex with calmodulin, is unknown. We previously determined the high-resolution structure of calmodulin in complex with the regulatory domain of the plasma-membrane Ca2+-ATPase ACA8 and revealed a bimodular mechanism of calcium control in eukaryotes. Here we show that activation of ACA8 by CaM involves large conformational changes. Combining advanced modeling of neutron scattering data acquired from stealth nanodiscs and native mass spectrometry with detailed dissection of binding constants, we present a structural model for the full-length ACA8 Ca2+ pump in its calmodulin-activated state illustrating a displacement of the regulatory domain from the core enzyme.

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Communications Biology
volume
1
pages
206 - 206
publisher
Nature Research
external identifiers
  • scopus:85066053657
  • pmid:30511020
ISSN
2399-3642
DOI
10.1038/s42003-018-0203-7
language
English
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no
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c479267a-7530-425e-9865-ba6c98600851
date added to LUP
2019-02-06 10:16:43
date last changed
2020-04-07 05:15:09
@article{c479267a-7530-425e-9865-ba6c98600851,
  abstract     = {<p>Plasma-membrane Ca2+-ATPases expel Ca2+ from the cytoplasm and are key regulators of Ca2+ homeostasis in eukaryotes. They are autoinhibited under low Ca2+ concentrations. Calmodulin (CaM)-binding to a unique regulatory domain releases the autoinhibition and activates the pump. However, the structural basis for this activation, including the overall structure of this calcium pump and its complex with calmodulin, is unknown. We previously determined the high-resolution structure of calmodulin in complex with the regulatory domain of the plasma-membrane Ca2+-ATPase ACA8 and revealed a bimodular mechanism of calcium control in eukaryotes. Here we show that activation of ACA8 by CaM involves large conformational changes. Combining advanced modeling of neutron scattering data acquired from stealth nanodiscs and native mass spectrometry with detailed dissection of binding constants, we present a structural model for the full-length ACA8 Ca2+ pump in its calmodulin-activated state illustrating a displacement of the regulatory domain from the core enzyme.</p>},
  author       = {Nitsche, Julius and Josts, Inokentijs and Heidemann, Johannes and Mertens, Haydyn D and Maric, Selma and Moulin, Martine and Haertlein, Michael and Busch, Sebastian and Forsyth, V Trevor and Svergun, Dmitri I and Uetrecht, Charlotte and Tidow, Henning},
  issn         = {2399-3642},
  language     = {eng},
  pages        = {206--206},
  publisher    = {Nature Research},
  series       = {Communications Biology},
  title        = {Structural basis for activation of plasma-membrane Ca2+-ATPase by calmodulin},
  url          = {http://dx.doi.org/10.1038/s42003-018-0203-7},
  doi          = {10.1038/s42003-018-0203-7},
  volume       = {1},
  year         = {2018},
}