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Sponge Phases and Nanoparticle Dispersions in Aqueous Mixtures of Mono- and Diglycerides

Valldeperas Badell, Maria LU ; Wiśniewska, Małgorzata; Ram-On, Maor; Kesselman, Ellina; Danino, Dganit; Nylander, Tommy LU and Barauskas, Justas LU (2016) In Langmuir 32(34). p.8650-8659
Abstract

The lipid liquid crystalline sponge phase (L3) has the advantages that it is a nanoscopically bicontinuous bilayer network able to accommodate large amounts of water and it is easy to manipulate due to its fluidity. This paper reports on the detailed characterization of L3 phases with water channels large enough to encapsulate bioactive macromolecules such as proteins. The aqueous phase behavior of a novel lipid mixture system, consisting of diglycerol monooleate (DGMO), and a mixture of mono-, di- and triglycerides (Capmul GMO-50) was studied. In addition, sponge-like nanoparticles (NPs) stabilized by Polysorbate 80 (P80) were prepared based on the DGMO/GMO-50 system, and their structure was correlated with the... (More)

The lipid liquid crystalline sponge phase (L3) has the advantages that it is a nanoscopically bicontinuous bilayer network able to accommodate large amounts of water and it is easy to manipulate due to its fluidity. This paper reports on the detailed characterization of L3 phases with water channels large enough to encapsulate bioactive macromolecules such as proteins. The aqueous phase behavior of a novel lipid mixture system, consisting of diglycerol monooleate (DGMO), and a mixture of mono-, di- and triglycerides (Capmul GMO-50) was studied. In addition, sponge-like nanoparticles (NPs) stabilized by Polysorbate 80 (P80) were prepared based on the DGMO/GMO-50 system, and their structure was correlated with the phase behavior of the corresponding bulk system. These NPs were characterized by dynamic light scattering (DLS), cryo-transmission electron microscopy (Cryo-TEM) and small angle X-ray scattering (SAXS) to determine their size, shape, and inner structure as a function of the DGMO/GMO-50 ratio. In addition, the effect of P80 as stabilizer was investigated. We found that the NPs have aqueous pores with diameters up to 13 nm, similar to the ones in the bulk phase.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Langmuir
volume
32
issue
34
pages
10 pages
publisher
The American Chemical Society
external identifiers
  • scopus:84984856534
  • wos:000382513900012
ISSN
0743-7463
DOI
10.1021/acs.langmuir.6b01356
language
English
LU publication?
yes
id
c4bc01d7-6f85-429c-973c-e063e38338eb
date added to LUP
2016-10-10 13:51:56
date last changed
2017-03-26 04:45:48
@article{c4bc01d7-6f85-429c-973c-e063e38338eb,
  abstract     = {<p>The lipid liquid crystalline sponge phase (L<sub>3</sub>) has the advantages that it is a nanoscopically bicontinuous bilayer network able to accommodate large amounts of water and it is easy to manipulate due to its fluidity. This paper reports on the detailed characterization of L<sub>3</sub> phases with water channels large enough to encapsulate bioactive macromolecules such as proteins. The aqueous phase behavior of a novel lipid mixture system, consisting of diglycerol monooleate (DGMO), and a mixture of mono-, di- and triglycerides (Capmul GMO-50) was studied. In addition, sponge-like nanoparticles (NPs) stabilized by Polysorbate 80 (P80) were prepared based on the DGMO/GMO-50 system, and their structure was correlated with the phase behavior of the corresponding bulk system. These NPs were characterized by dynamic light scattering (DLS), cryo-transmission electron microscopy (Cryo-TEM) and small angle X-ray scattering (SAXS) to determine their size, shape, and inner structure as a function of the DGMO/GMO-50 ratio. In addition, the effect of P80 as stabilizer was investigated. We found that the NPs have aqueous pores with diameters up to 13 nm, similar to the ones in the bulk phase.</p>},
  author       = {Valldeperas Badell, Maria and Wiśniewska, Małgorzata and Ram-On, Maor and Kesselman, Ellina and Danino, Dganit and Nylander, Tommy and Barauskas, Justas},
  issn         = {0743-7463},
  language     = {eng},
  month        = {08},
  number       = {34},
  pages        = {8650--8659},
  publisher    = {The American Chemical Society},
  series       = {Langmuir},
  title        = {Sponge Phases and Nanoparticle Dispersions in Aqueous Mixtures of Mono- and Diglycerides},
  url          = {http://dx.doi.org/10.1021/acs.langmuir.6b01356},
  volume       = {32},
  year         = {2016},
}