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The use of a recombinant lentiviral vector for ex vivo gene transfer into the rat CNS

Englund, Ulrica LU ; Ericson, Cecilia LU ; Rosenblad, Carl LU ; Mandel, Ronald J.; Trono, Didier; Wictorin, Klas LU and Lundberg, Cecilia LU (2000) In NeuroReport 11(18). p.3973-3977
Abstract

A major obstacle in ex vivo gene transfer has been the loss of transgene expression soon after implantation of the grafted transduced cells. Recently, a lentiviral vector system has been developed which has proven to express high levels of transgenes in vivo after direct injection into the tissue. In this study, we have investigated the use of such a vector for ex vivo gene transfer to the brain. A number of neural cell types were found to be permissive to transduction by the lentiviral vector in vitro and a majority of them expressed the transgene after transplantation to the rat brain. Transgene expression was detected up to 8 weeks post-grafting. These findings suggest that recombinant lentiviral vectors may be used for further... (More)

A major obstacle in ex vivo gene transfer has been the loss of transgene expression soon after implantation of the grafted transduced cells. Recently, a lentiviral vector system has been developed which has proven to express high levels of transgenes in vivo after direct injection into the tissue. In this study, we have investigated the use of such a vector for ex vivo gene transfer to the brain. A number of neural cell types were found to be permissive to transduction by the lentiviral vector in vitro and a majority of them expressed the transgene after transplantation to the rat brain. Transgene expression was detected up to 8 weeks post-grafting. These findings suggest that recombinant lentiviral vectors may be used for further development of ex vivo gene therapy protocols to the CNS. © 2000 Lippincott Williams & Wilkins.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Ex vivo gene transfer, Green fluorescent protein, Lentiviral vector
in
NeuroReport
volume
11
issue
18
pages
5 pages
publisher
Lippincott Williams & Wilkins
external identifiers
  • scopus:0034524787
ISSN
0959-4965
language
English
LU publication?
yes
id
c554bdf1-82f3-4f8f-bd29-0e1b5d4ac9bc
date added to LUP
2016-11-14 12:22:17
date last changed
2017-01-01 08:39:32
@article{c554bdf1-82f3-4f8f-bd29-0e1b5d4ac9bc,
  abstract     = {<p>A major obstacle in ex vivo gene transfer has been the loss of transgene expression soon after implantation of the grafted transduced cells. Recently, a lentiviral vector system has been developed which has proven to express high levels of transgenes in vivo after direct injection into the tissue. In this study, we have investigated the use of such a vector for ex vivo gene transfer to the brain. A number of neural cell types were found to be permissive to transduction by the lentiviral vector in vitro and a majority of them expressed the transgene after transplantation to the rat brain. Transgene expression was detected up to 8 weeks post-grafting. These findings suggest that recombinant lentiviral vectors may be used for further development of ex vivo gene therapy protocols to the CNS. © 2000 Lippincott Williams &amp; Wilkins.</p>},
  author       = {Englund, Ulrica and Ericson, Cecilia and Rosenblad, Carl and Mandel, Ronald J. and Trono, Didier and Wictorin, Klas and Lundberg, Cecilia},
  issn         = {0959-4965},
  keyword      = {Ex vivo gene transfer,Green fluorescent protein,Lentiviral vector},
  language     = {eng},
  number       = {18},
  pages        = {3973--3977},
  publisher    = {Lippincott Williams & Wilkins},
  series       = {NeuroReport},
  title        = {The use of a recombinant lentiviral vector for ex vivo gene transfer into the rat CNS},
  volume       = {11},
  year         = {2000},
}