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Subcritical water extraction and characterization of bioactive compounds from Haematococcus pluvialis microalga

Rodríguez-Meizoso, I. LU ; Jaime, Laura; Santoyo, Susana; Señoráns, F. J.; Cifuentes, Alejandro and Ibáñez, Elena (2010) In Journal of Pharmaceutical and Biomedical Analysis 51(2). p.456-463
Abstract

In this work, extraction and characterization of compounds with antioxidant and antimicrobial activity from Haematococcus pluvialis microalga in red phase have been carried out. To do this, subcritical water extraction (SWE) has been combined with analytical techniques such as HPLC-DAD, HPLC-QqQ-MS and GC-MS and in vitro assays (i.e., for antioxidant and antimicrobial activity). The effect of the extraction temperature (50, 100, 150 and 200 °C) and solvent polarity has been studied in terms of yield and activity of the extracts. Results demonstrate that the extraction temperature has a positive influence in the extraction yield and antioxidant activity. Thus, the extraction yield achieved with this process was higher than 30% of dry... (More)

In this work, extraction and characterization of compounds with antioxidant and antimicrobial activity from Haematococcus pluvialis microalga in red phase have been carried out. To do this, subcritical water extraction (SWE) has been combined with analytical techniques such as HPLC-DAD, HPLC-QqQ-MS and GC-MS and in vitro assays (i.e., for antioxidant and antimicrobial activity). The effect of the extraction temperature (50, 100, 150 and 200 °C) and solvent polarity has been studied in terms of yield and activity of the extracts. Results demonstrate that the extraction temperature has a positive influence in the extraction yield and antioxidant activity. Thus, the extraction yield achieved with this process was higher than 30% of dry weight at 200 °C as extraction temperature. Moreover, the extract obtained at 200 °C presented the highest antioxidant activity by far, while temperature does not seem to significatively affect the antimicrobial activity. Chemical composition was determined by HPLC-DAD, HPLC-QqQ-MS and GC-MS. Short chain fatty acids turned out to be responsible of the antimicrobial activity, whereas the antioxidant activity was correlated to vitamin E (present exclusively in the 200 °C extract), together with simple phenols, caramelization products and possible Maillard reaction products obtained during the extraction at high temperatures.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Antimicrobials, Antioxidants, Bioactive compounds, Chemical characterization, Haematococcus pluvialis, Microalgae, Subcritical water extraction
in
Journal of Pharmaceutical and Biomedical Analysis
volume
51
issue
2
pages
8 pages
publisher
Elsevier
external identifiers
  • scopus:70349814652
ISSN
0731-7085
DOI
10.1016/j.jpba.2009.03.014
language
English
LU publication?
no
id
c5675425-b1d2-4abe-b332-dac900a7b4d5
date added to LUP
2017-04-10 10:26:52
date last changed
2018-12-02 04:49:34
@article{c5675425-b1d2-4abe-b332-dac900a7b4d5,
  abstract     = {<p>In this work, extraction and characterization of compounds with antioxidant and antimicrobial activity from Haematococcus pluvialis microalga in red phase have been carried out. To do this, subcritical water extraction (SWE) has been combined with analytical techniques such as HPLC-DAD, HPLC-QqQ-MS and GC-MS and in vitro assays (i.e., for antioxidant and antimicrobial activity). The effect of the extraction temperature (50, 100, 150 and 200 °C) and solvent polarity has been studied in terms of yield and activity of the extracts. Results demonstrate that the extraction temperature has a positive influence in the extraction yield and antioxidant activity. Thus, the extraction yield achieved with this process was higher than 30% of dry weight at 200 °C as extraction temperature. Moreover, the extract obtained at 200 °C presented the highest antioxidant activity by far, while temperature does not seem to significatively affect the antimicrobial activity. Chemical composition was determined by HPLC-DAD, HPLC-QqQ-MS and GC-MS. Short chain fatty acids turned out to be responsible of the antimicrobial activity, whereas the antioxidant activity was correlated to vitamin E (present exclusively in the 200 °C extract), together with simple phenols, caramelization products and possible Maillard reaction products obtained during the extraction at high temperatures.</p>},
  author       = {Rodríguez-Meizoso, I. and Jaime, Laura and Santoyo, Susana and Señoráns, F. J. and Cifuentes, Alejandro and Ibáñez, Elena},
  issn         = {0731-7085},
  keyword      = {Antimicrobials,Antioxidants,Bioactive compounds,Chemical characterization,Haematococcus pluvialis,Microalgae,Subcritical water extraction},
  language     = {eng},
  month        = {01},
  number       = {2},
  pages        = {456--463},
  publisher    = {Elsevier},
  series       = {Journal of Pharmaceutical and Biomedical Analysis},
  title        = {Subcritical water extraction and characterization of bioactive compounds from Haematococcus pluvialis microalga},
  url          = {http://dx.doi.org/10.1016/j.jpba.2009.03.014},
  volume       = {51},
  year         = {2010},
}