Surface complexation of DNA with insoluble monolayers. Influence of divalent counterions
(2005) In Langmuir 21(5). p.1900-1907- Abstract
- DNA interacts with insoluble monolayers made of cationic amphiphiles as well as with monolayers of zwitterionic lipids in the presence of divalent ions. Binding to dioctadecyldimethylammonium bromide (DODAB) or distearoyl-sn-glycero-3-phosphocholine (DSPC) monolayers in the presence of calcium is accompanied by monolayer expansion. For the positively charged DODAB monolayer, this causes a decrease of surface potential, while an increase is observed for the DSPC monolayers. Binding to dipalmitoyl-sn-glycero-3-phosphocholine preserves most of the liquid expanded-liquid condensed coexistence region. The liquid condensed domains adopt an elongated morphology in the presence of DNA, especially in the presence of calcium. The interaction of DNA... (More)
- DNA interacts with insoluble monolayers made of cationic amphiphiles as well as with monolayers of zwitterionic lipids in the presence of divalent ions. Binding to dioctadecyldimethylammonium bromide (DODAB) or distearoyl-sn-glycero-3-phosphocholine (DSPC) monolayers in the presence of calcium is accompanied by monolayer expansion. For the positively charged DODAB monolayer, this causes a decrease of surface potential, while an increase is observed for the DSPC monolayers. Binding to dipalmitoyl-sn-glycero-3-phosphocholine preserves most of the liquid expanded-liquid condensed coexistence region. The liquid condensed domains adopt an elongated morphology in the presence of DNA, especially in the presence of calcium. The interaction of DNA with phospholipid monolayers is ion specific: the presence of calcium leads to a stronger interaction than magnesium and barium. These results were confirmed by bulk complexation studies. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/157477
- author
- McLoughlin, D ; Dias, R ; Lindman, Björn LU ; Cardenas, M ; Nylander, Tommy LU ; Dawson, K ; Miguel, M and Langevin, D
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Langmuir
- volume
- 21
- issue
- 5
- pages
- 1900 - 1907
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- pmid:15723487
- wos:000227193500037
- scopus:14844336901
- pmid:15723487
- ISSN
- 0743-7463
- DOI
- 10.1021/la047700s
- language
- English
- LU publication?
- yes
- id
- c5faf7a4-29ef-4574-bb89-6708c52d0ad5 (old id 157477)
- date added to LUP
- 2016-04-01 12:37:29
- date last changed
- 2022-03-06 02:05:45
@article{c5faf7a4-29ef-4574-bb89-6708c52d0ad5, abstract = {{DNA interacts with insoluble monolayers made of cationic amphiphiles as well as with monolayers of zwitterionic lipids in the presence of divalent ions. Binding to dioctadecyldimethylammonium bromide (DODAB) or distearoyl-sn-glycero-3-phosphocholine (DSPC) monolayers in the presence of calcium is accompanied by monolayer expansion. For the positively charged DODAB monolayer, this causes a decrease of surface potential, while an increase is observed for the DSPC monolayers. Binding to dipalmitoyl-sn-glycero-3-phosphocholine preserves most of the liquid expanded-liquid condensed coexistence region. The liquid condensed domains adopt an elongated morphology in the presence of DNA, especially in the presence of calcium. The interaction of DNA with phospholipid monolayers is ion specific: the presence of calcium leads to a stronger interaction than magnesium and barium. These results were confirmed by bulk complexation studies.}}, author = {{McLoughlin, D and Dias, R and Lindman, Björn and Cardenas, M and Nylander, Tommy and Dawson, K and Miguel, M and Langevin, D}}, issn = {{0743-7463}}, language = {{eng}}, number = {{5}}, pages = {{1900--1907}}, publisher = {{The American Chemical Society (ACS)}}, series = {{Langmuir}}, title = {{Surface complexation of DNA with insoluble monolayers. Influence of divalent counterions}}, url = {{http://dx.doi.org/10.1021/la047700s}}, doi = {{10.1021/la047700s}}, volume = {{21}}, year = {{2005}}, }