Increased perfusion pressure enhances the expression of endothelin (ETB) and angiotensin II (AT1, AT2) receptors in rat mesenteric artery smooth muscle cells.
(2009) In Blood Pressure 18(1). p.78-85- Abstract
- In the present study, we hypothesized that changes in perfusion pressure result in altered expression of mRNA and protein encoding for the ETA-, ETB-, AT1- and AT2-receptors in rat mesenteric vessels. Segments of the rat mesenteric artery were cannulated with glass micropipettes, pressurized and luminally perfused in a perfusion chamber. After either exposure to no ("organ culture" (0 mmHg)), normal (85/75 mmHg) or high pressure (160/150 mmHg) at constant flow for 1-17 h, the vessel segments were snap frozen and real-time polymerase chain reaction was performed to quantify the ET- and AT-receptor mRNA content, or immersed in a fixative solution, dehydrated, frozen, cut in a cryostat and immunohistology stained for ET- and AT-receptor... (More)
- In the present study, we hypothesized that changes in perfusion pressure result in altered expression of mRNA and protein encoding for the ETA-, ETB-, AT1- and AT2-receptors in rat mesenteric vessels. Segments of the rat mesenteric artery were cannulated with glass micropipettes, pressurized and luminally perfused in a perfusion chamber. After either exposure to no ("organ culture" (0 mmHg)), normal (85/75 mmHg) or high pressure (160/150 mmHg) at constant flow for 1-17 h, the vessel segments were snap frozen and real-time polymerase chain reaction was performed to quantify the ET- and AT-receptor mRNA content, or immersed in a fixative solution, dehydrated, frozen, cut in a cryostat and immunohistology stained for ET- and AT-receptor protein. The mRNA expressions of ETB and of AT2 were significantly enhanced in vessels exposed to high perfusion pressure, compared with normal and no perfusion pressure at 4 h. In concordance, AT1-, AT2- and ETB-receptor proteins were up-regulated at 17 h of high perfusion pressure. In conclusion, the results from our rat perfusion model suggest a more important role of shear stress than pure pressure alone and may serve as a surrogate model for studies designed to investigate hypertension mechanisms. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1392291
- author
- Lindstedt, Isak ; Xu, Cang-Bao LU ; Zhang, Yaping LU and Edvinsson, Lars LU
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Blood Pressure
- volume
- 18
- issue
- 1
- pages
- 78 - 85
- publisher
- Taylor & Francis
- external identifiers
-
- wos:000265291000013
- pmid:19353416
- scopus:67650124133
- pmid:19353416
- ISSN
- 0803-7051
- DOI
- 10.1080/08037050902850184
- language
- English
- LU publication?
- yes
- id
- c7127739-4110-4c6b-8dd7-334ec6c3219a (old id 1392291)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/19353416?dopt=Abstract
- date added to LUP
- 2016-04-04 07:27:22
- date last changed
- 2024-10-12 14:27:42
@article{c7127739-4110-4c6b-8dd7-334ec6c3219a,
abstract = {{In the present study, we hypothesized that changes in perfusion pressure result in altered expression of mRNA and protein encoding for the ETA-, ETB-, AT1- and AT2-receptors in rat mesenteric vessels. Segments of the rat mesenteric artery were cannulated with glass micropipettes, pressurized and luminally perfused in a perfusion chamber. After either exposure to no ("organ culture" (0 mmHg)), normal (85/75 mmHg) or high pressure (160/150 mmHg) at constant flow for 1-17 h, the vessel segments were snap frozen and real-time polymerase chain reaction was performed to quantify the ET- and AT-receptor mRNA content, or immersed in a fixative solution, dehydrated, frozen, cut in a cryostat and immunohistology stained for ET- and AT-receptor protein. The mRNA expressions of ETB and of AT2 were significantly enhanced in vessels exposed to high perfusion pressure, compared with normal and no perfusion pressure at 4 h. In concordance, AT1-, AT2- and ETB-receptor proteins were up-regulated at 17 h of high perfusion pressure. In conclusion, the results from our rat perfusion model suggest a more important role of shear stress than pure pressure alone and may serve as a surrogate model for studies designed to investigate hypertension mechanisms.}},
author = {{Lindstedt, Isak and Xu, Cang-Bao and Zhang, Yaping and Edvinsson, Lars}},
issn = {{0803-7051}},
language = {{eng}},
number = {{1}},
pages = {{78--85}},
publisher = {{Taylor & Francis}},
series = {{Blood Pressure}},
title = {{Increased perfusion pressure enhances the expression of endothelin (ETB) and angiotensin II (AT1, AT2) receptors in rat mesenteric artery smooth muscle cells.}},
url = {{http://dx.doi.org/10.1080/08037050902850184}},
doi = {{10.1080/08037050902850184}},
volume = {{18}},
year = {{2009}},
}