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Analysis of Anthocyanin-Less 2 Diversity in Barley Reveals a Specific Allele to Cause Purple-Colored Grains

Shoeva, Olesya Y. ; Zedgenizova, Varvara D. ; Egorova, Anastasiya A. ; Gerasimova, Sophia V. ; Kukoeva, Tatjana V. ; Vasiliev, Gennady V. ; Kovaleva, Olga N. ; Zakhrabekova, Shakhira LU ; Hansson, Mats LU and Hertig, Christian W. , et al. (2025) In Journal of Agricultural and Food Chemistry 73(40). p.25491-25504
Abstract

The purple color of the barley (Hordeum vulgare L.) grain is attributed to anthocyanins which are beneficial for human health. Synthesis of these pigments in both grain and vegetative tissues is controlled by ANT1 and ANT2 that belong to the families R2R3-MYB and bHLH of transcription factors, respectively. Here, we investigated the role of the Ant2 gene in pigmentation of grains compared to vegetative tissue. After screening of 504 barley accessions, six relevant alleles of Ant2 were identified. These involve distinct insertions in the promoter and/or in intron 6. Allele Ant2.l was found in all purple-grained barley accessions and was more strongly expressed compared to the alleles present in the other lines. The crucial role of Ant2.l... (More)

The purple color of the barley (Hordeum vulgare L.) grain is attributed to anthocyanins which are beneficial for human health. Synthesis of these pigments in both grain and vegetative tissues is controlled by ANT1 and ANT2 that belong to the families R2R3-MYB and bHLH of transcription factors, respectively. Here, we investigated the role of the Ant2 gene in pigmentation of grains compared to vegetative tissue. After screening of 504 barley accessions, six relevant alleles of Ant2 were identified. These involve distinct insertions in the promoter and/or in intron 6. Allele Ant2.l was found in all purple-grained barley accessions and was more strongly expressed compared to the alleles present in the other lines. The crucial role of Ant2.l in grain pigmentation was validated by targeted mutagenesis using RNA-guided endonuclease Cas9. It is further shown that the other Ant2 alleles do not affect grain pigmentation while being essential for pigmentation of vegetative tissues.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
CRISPR/cas, genome editing, Hordeum vulgare L., mutant
in
Journal of Agricultural and Food Chemistry
volume
73
issue
40
pages
14 pages
publisher
The American Chemical Society (ACS)
external identifiers
  • pmid:40997767
  • scopus:105017982946
ISSN
0021-8561
DOI
10.1021/acs.jafc.5c05032
language
English
LU publication?
yes
id
c801c43e-4bca-4d17-9340-e75de7bae029
date added to LUP
2025-11-24 14:54:30
date last changed
2025-12-08 16:17:13
@article{c801c43e-4bca-4d17-9340-e75de7bae029,
  abstract     = {{<p>The purple color of the barley (Hordeum vulgare L.) grain is attributed to anthocyanins which are beneficial for human health. Synthesis of these pigments in both grain and vegetative tissues is controlled by ANT1 and ANT2 that belong to the families R2R3-MYB and bHLH of transcription factors, respectively. Here, we investigated the role of the Ant2 gene in pigmentation of grains compared to vegetative tissue. After screening of 504 barley accessions, six relevant alleles of Ant2 were identified. These involve distinct insertions in the promoter and/or in intron 6. Allele Ant2.l was found in all purple-grained barley accessions and was more strongly expressed compared to the alleles present in the other lines. The crucial role of Ant2.l in grain pigmentation was validated by targeted mutagenesis using RNA-guided endonuclease Cas9. It is further shown that the other Ant2 alleles do not affect grain pigmentation while being essential for pigmentation of vegetative tissues.</p>}},
  author       = {{Shoeva, Olesya Y. and Zedgenizova, Varvara D. and Egorova, Anastasiya A. and Gerasimova, Sophia V. and Kukoeva, Tatjana V. and Vasiliev, Gennady V. and Kovaleva, Olga N. and Zakhrabekova, Shakhira and Hansson, Mats and Hertig, Christian W. and Hoffie, Iris and Kumlehn, Jochen and Khlestkina, Elena}},
  issn         = {{0021-8561}},
  keywords     = {{CRISPR/cas; genome editing; Hordeum vulgare L.; mutant}},
  language     = {{eng}},
  number       = {{40}},
  pages        = {{25491--25504}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{Journal of Agricultural and Food Chemistry}},
  title        = {{Analysis of Anthocyanin-Less 2 Diversity in Barley Reveals a Specific Allele to Cause Purple-Colored Grains}},
  url          = {{http://dx.doi.org/10.1021/acs.jafc.5c05032}},
  doi          = {{10.1021/acs.jafc.5c05032}},
  volume       = {{73}},
  year         = {{2025}},
}