Evaluation of recombinant human IGF‑1/IGFBP‑3 on intraventricular hemorrhage prevention and survival in the preterm rabbit pup model
(2023) In Scientific Reports 13. p.1-12- Abstract
- Insulin-like growth factor-1 (IGF-1) is essential for normal brain development and regulates processes
of vascular maturation. The pathogenesis of intraventricular hemorrhage (IVH) relates to the fragility
of the immature capillaries in the germinal matrix, and its inability to resist fluctuations in cerebral
blood flow. In this work, using different experimental setups, we aimed to (i) establish an optimal
time-point for glycerol-induction of IVH in relation to time-point of recombinant human (rh) IGF-1/
rhIGFBP-3 administration, and (ii) to evaluate the effects of a physiologic replacement dose of
rhIGF-1/rhIGFBP-3 on prevention of IVH and survival in the preterm rabbit pup. The presence of IVH
was evaluated... (More) - Insulin-like growth factor-1 (IGF-1) is essential for normal brain development and regulates processes
of vascular maturation. The pathogenesis of intraventricular hemorrhage (IVH) relates to the fragility
of the immature capillaries in the germinal matrix, and its inability to resist fluctuations in cerebral
blood flow. In this work, using different experimental setups, we aimed to (i) establish an optimal
time-point for glycerol-induction of IVH in relation to time-point of recombinant human (rh) IGF-1/
rhIGFBP-3 administration, and (ii) to evaluate the effects of a physiologic replacement dose of
rhIGF-1/rhIGFBP-3 on prevention of IVH and survival in the preterm rabbit pup. The presence of IVH
was evaluated using high-frequency ultrasound and post-mortem examinations. In the first part
of the study, the highest incidence of IVH (> 60%), occurred when glycerol was administered at the
earliest timepoint, e.g., 6 h after birth. At later time-points (18 and 24 h) the incidence decreased
substantially. In the second part of the study, the incidence of IVH and mortality rate following rhIGF-1/rhIGFBP-3 administration was not statistically different compared to vehicle treated animals.
To evaluate the importance of maintaining intrauterine serum levels of IGF-1 following preterm birth,
as reported in human interventional studies, additional studies are needed to further characterize and
establish the potential of rhIGF-1/rhIGFBP-3 in reducing the prevalence of IVH and improving survival
in the preterm rabbit pup. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/c8037fea-16ab-4c6f-b7ea-7b0df0cc9499
- author
- organization
- publishing date
- 2023
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Scientific Reports
- volume
- 13
- article number
- 19847
- pages
- 1 - 12
- publisher
- Nature Publishing Group
- external identifiers
-
- pmid:37963901
- scopus:85176401894
- ISSN
- 2045-2322
- DOI
- 10.1038/s41598-023-46611-0
- language
- English
- LU publication?
- yes
- id
- c8037fea-16ab-4c6f-b7ea-7b0df0cc9499
- date added to LUP
- 2023-12-01 09:22:56
- date last changed
- 2023-12-02 04:06:40
@article{c8037fea-16ab-4c6f-b7ea-7b0df0cc9499, abstract = {{Insulin-like growth factor-1 (IGF-1) is essential for normal brain development and regulates processes<br/>of vascular maturation. The pathogenesis of intraventricular hemorrhage (IVH) relates to the fragility<br/>of the immature capillaries in the germinal matrix, and its inability to resist fluctuations in cerebral<br/>blood flow. In this work, using different experimental setups, we aimed to (i) establish an optimal<br/>time-point for glycerol-induction of IVH in relation to time-point of recombinant human (rh) IGF-1/<br/>rhIGFBP-3 administration, and (ii) to evaluate the effects of a physiologic replacement dose of<br/>rhIGF-1/rhIGFBP-3 on prevention of IVH and survival in the preterm rabbit pup. The presence of IVH<br/>was evaluated using high-frequency ultrasound and post-mortem examinations. In the first part<br/>of the study, the highest incidence of IVH (> 60%), occurred when glycerol was administered at the<br/>earliest timepoint, e.g., 6 h after birth. At later time-points (18 and 24 h) the incidence decreased<br/>substantially. In the second part of the study, the incidence of IVH and mortality rate following rhIGF-1/rhIGFBP-3 administration was not statistically different compared to vehicle treated animals.<br/>To evaluate the importance of maintaining intrauterine serum levels of IGF-1 following preterm birth,<br/>as reported in human interventional studies, additional studies are needed to further characterize and<br/>establish the potential of rhIGF-1/rhIGFBP-3 in reducing the prevalence of IVH and improving survival<br/>in the preterm rabbit pup.}}, author = {{Ekström, Claes and Ortenlöf, Niklas and Kristiansson, Amanda and Holmqvist, Bo and Jungner, Åsa and Vallius, Suvi and Wang, Xiaoyang and Hellström, Ann and Barton, Norman W. and Carey, Galen and Ley, David and Gram, Magnus}}, issn = {{2045-2322}}, language = {{eng}}, pages = {{1--12}}, publisher = {{Nature Publishing Group}}, series = {{Scientific Reports}}, title = {{Evaluation of recombinant human IGF‑1/IGFBP‑3 on intraventricular hemorrhage prevention and survival in the preterm rabbit pup model}}, url = {{http://dx.doi.org/10.1038/s41598-023-46611-0}}, doi = {{10.1038/s41598-023-46611-0}}, volume = {{13}}, year = {{2023}}, }