A Probing Feeding Strategy for E. coli Cultivations
(1999) Abstract book of 217th ACS National Meeting- Abstract
- Accumulation of acetate tends to reduce growth and recombinant protein production in E. coli cultivations. Formation of acetate can be avoided by a proper substrate feeding strategy, but most strategies require considerable process knowledge to work well. A feedback strategy is presented that requires minimal a priori information. The key idea is to exploit a characteristic change in the cell metabolism at the onset of acetate formation. By superimposing short pulses in the substrate feed rate, on-line detection of acetate formation can be made using a standard dissolved oxygen sensor. A simple feedback algorithm is then used to adjust the feed rate to avoid acetate formation while maintaining a high growth rate. The strategy was used in... (More)
- Accumulation of acetate tends to reduce growth and recombinant protein production in E. coli cultivations. Formation of acetate can be avoided by a proper substrate feeding strategy, but most strategies require considerable process knowledge to work well. A feedback strategy is presented that requires minimal a priori information. The key idea is to exploit a characteristic change in the cell metabolism at the onset of acetate formation. By superimposing short pulses in the substrate feed rate, on-line detection of acetate formation can be made using a standard dissolved oxygen sensor. A simple feedback algorithm is then used to adjust the feed rate to avoid acetate formation while maintaining a high growth rate. The strategy was used in fed-batch cultivations of recombinant E. coli. Acetate accumulation was avoided and a good production of recombinant protein could be achieved at the first attempt for each genetic construction. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/8516719
- author
- Axelsson, Jan Peter ; Åkesson, Mats LU and Hagander, Per LU
- organization
- publishing date
- 1999
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- host publication
- Abstract book of 217th ACS National Meeting
- conference name
- Abstract book of 217th ACS National Meeting
- conference dates
- 1999-03-15
- language
- English
- LU publication?
- yes
- id
- c85c97eb-a478-4e81-a789-a1838b0f62c5 (old id 8516719)
- date added to LUP
- 2016-04-04 13:34:35
- date last changed
- 2018-11-21 21:14:53
@inproceedings{c85c97eb-a478-4e81-a789-a1838b0f62c5, abstract = {{Accumulation of acetate tends to reduce growth and recombinant protein production in E. coli cultivations. Formation of acetate can be avoided by a proper substrate feeding strategy, but most strategies require considerable process knowledge to work well. A feedback strategy is presented that requires minimal a priori information. The key idea is to exploit a characteristic change in the cell metabolism at the onset of acetate formation. By superimposing short pulses in the substrate feed rate, on-line detection of acetate formation can be made using a standard dissolved oxygen sensor. A simple feedback algorithm is then used to adjust the feed rate to avoid acetate formation while maintaining a high growth rate. The strategy was used in fed-batch cultivations of recombinant E. coli. Acetate accumulation was avoided and a good production of recombinant protein could be achieved at the first attempt for each genetic construction.}}, author = {{Axelsson, Jan Peter and Åkesson, Mats and Hagander, Per}}, booktitle = {{Abstract book of 217th ACS National Meeting}}, language = {{eng}}, title = {{A Probing Feeding Strategy for <em>E. coli</em> Cultivations}}, year = {{1999}}, }