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Highly efficient acoustophoretic single cell-supernatant separation inside nanoliter droplets

Gerlt, Michael LU orcid ; Haidas, Dominik ; Ratschat, Alexandre ; Suter, Philipp ; Dittrich, Petra and Dual, Jürg (2020) 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 In MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences p.260-261
Abstract

This contribution reports a novel method for cell-supernatant separation inside nanoliter droplets with separation efficiencies for single cells of 100% at flow speeds of 2 mm/s. Cells are focused in nanoliter droplets using bulk acoustic waves before the droplet is split at a bifurcation into a supernatant- and a cell-containing droplet. The method enables upconcentration of cells, cell washing, and the separation of supernatant.

Please use this url to cite or link to this publication:
author
; ; ; ; and
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Cell-supernatant separation, Intra droplet manipulation, Single-cell
host publication
MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
series title
MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
pages
2 pages
publisher
Chemical and Biological Microsystems Society
conference name
24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
conference location
Virtual, Online
conference dates
2020-10-04 - 2020-10-09
external identifiers
  • scopus:85098292067
ISBN
9781733419017
language
English
LU publication?
no
additional info
Publisher Copyright: © 2020 CBMS-0001
id
c8dc7938-23a8-4565-9c6f-3efe28ad897c
date added to LUP
2024-07-01 14:18:07
date last changed
2024-07-11 14:51:31
@inproceedings{c8dc7938-23a8-4565-9c6f-3efe28ad897c,
  abstract     = {{<p>This contribution reports a novel method for cell-supernatant separation inside nanoliter droplets with separation efficiencies for single cells of 100% at flow speeds of 2 mm/s. Cells are focused in nanoliter droplets using bulk acoustic waves before the droplet is split at a bifurcation into a supernatant- and a cell-containing droplet. The method enables upconcentration of cells, cell washing, and the separation of supernatant.</p>}},
  author       = {{Gerlt, Michael and Haidas, Dominik and Ratschat, Alexandre and Suter, Philipp and Dittrich, Petra and Dual, Jürg}},
  booktitle    = {{MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences}},
  isbn         = {{9781733419017}},
  keywords     = {{Cell-supernatant separation; Intra droplet manipulation; Single-cell}},
  language     = {{eng}},
  pages        = {{260--261}},
  publisher    = {{Chemical and Biological Microsystems Society}},
  series       = {{MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences}},
  title        = {{Highly efficient acoustophoretic single cell-supernatant separation inside nanoliter droplets}},
  year         = {{2020}},
}