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Bacillus subtilis cytochrome oxidase mutants: biochemical analysis and genetic evidence for two aa3-type oxidases

van der Oost, John; von Wachenfeldt, Claes LU ; Hederstedt, Lars LU and Saraste, M (1991) In Molecular Microbiology 5(8). p.2063-2072
Abstract
The ctaBCDEF genes coding for cytochrome c oxidase were found to reside adjacent to a regulatory gene ctaA at 127-degrees on the Bacillus subtilis chromosome. The structural genes for subunits I and II, ctaD and ctaC, were deleted by gene-replacement using a phleomycin-resistance marker. The mutant was unable to oxidize N,N,N',N'-tetramethyl-p-phenylenediamine and oxidized cytochrome c at a significantly lower rate. Absorption spectra of the mutant and wild-type membranes confirmed the presence of two haem A-containing enzymes in B. subtilis. Another mutant, with a spontaneous deletion upstream from ctaC, was found to express neither of these enzymes. Radioactive haem-labelling was used to identify subunit 11, which contains a haem C, and... (More)
The ctaBCDEF genes coding for cytochrome c oxidase were found to reside adjacent to a regulatory gene ctaA at 127-degrees on the Bacillus subtilis chromosome. The structural genes for subunits I and II, ctaD and ctaC, were deleted by gene-replacement using a phleomycin-resistance marker. The mutant was unable to oxidize N,N,N',N'-tetramethyl-p-phenylenediamine and oxidized cytochrome c at a significantly lower rate. Absorption spectra of the mutant and wild-type membranes confirmed the presence of two haem A-containing enzymes in B. subtilis. Another mutant, with a spontaneous deletion upstream from ctaC, was found to express neither of these enzymes. Radioactive haem-labelling was used to identify subunit 11, which contains a haem C, and cytochrome c-550 among the membrane-bound c-type cytochromes of B. subtilis. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Molecular Microbiology
volume
5
issue
8
pages
2063 - 2072
publisher
Wiley-Blackwell
ISSN
1365-2958
DOI
10.1111/j.1365-2958.1991.tb00829.x
language
English
LU publication?
yes
id
c9936668-1503-4a1e-8f4e-5f3ff34174bb
date added to LUP
2017-07-18 10:36:36
date last changed
2017-08-23 14:57:23
@article{c9936668-1503-4a1e-8f4e-5f3ff34174bb,
  abstract     = {The ctaBCDEF genes coding for cytochrome c oxidase were found to reside adjacent to a regulatory gene ctaA at 127-degrees on the Bacillus subtilis chromosome. The structural genes for subunits I and II, ctaD and ctaC, were deleted by gene-replacement using a phleomycin-resistance marker. The mutant was unable to oxidize N,N,N',N'-tetramethyl-p-phenylenediamine and oxidized cytochrome c at a significantly lower rate. Absorption spectra of the mutant and wild-type membranes confirmed the presence of two haem A-containing enzymes in B. subtilis. Another mutant, with a spontaneous deletion upstream from ctaC, was found to express neither of these enzymes. Radioactive haem-labelling was used to identify subunit 11, which contains a haem C, and cytochrome c-550 among the membrane-bound c-type cytochromes of B. subtilis.},
  author       = {van der Oost, John and von Wachenfeldt, Claes and Hederstedt, Lars and Saraste, M},
  issn         = {1365-2958},
  language     = {eng},
  number       = {8},
  pages        = {2063--2072},
  publisher    = {Wiley-Blackwell},
  series       = {Molecular Microbiology},
  title        = {<em>Bacillus subtilis</em> cytochrome oxidase mutants: biochemical analysis and genetic evidence for two aa<sub>3</sub>-type oxidases},
  url          = {http://dx.doi.org/10.1111/j.1365-2958.1991.tb00829.x},
  volume       = {5},
  year         = {1991},
}