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Characterization of Gluconobacter oxydans immobilized in calcium alginate

Adlercreutz, Patrick LU orcid ; Holst, Olle LU and Mattiasson, Bo LU (1985) In Applied Microbiology and Biotechnology 22(1). p.1-7
Abstract

Gluconobacter oxydans cells were immobilized in calcium alginate and the preparation was used for the oxidation of glycerol to dihydroxyacetone. The characterization was done according to the guidelines given by the Working Party on Immobilized Biocatalysts of the European Federation of Biotechnology. The pH optimum of the preparation was found to be 5.0 and the temperature optimum was 40°C. However, the operational stability was better at 30°C. The glycerol concentration required to obtain half the maximal reaction rate was about 5 mM for both immobilized and free cells. At low concentrations of glycerol and high concentrations of dihydroxyacetone a slight inhibition was noted. No loss of activity of the immobilized preparation was... (More)

Gluconobacter oxydans cells were immobilized in calcium alginate and the preparation was used for the oxidation of glycerol to dihydroxyacetone. The characterization was done according to the guidelines given by the Working Party on Immobilized Biocatalysts of the European Federation of Biotechnology. The pH optimum of the preparation was found to be 5.0 and the temperature optimum was 40°C. However, the operational stability was better at 30°C. The glycerol concentration required to obtain half the maximal reaction rate was about 5 mM for both immobilized and free cells. At low concentrations of glycerol and high concentrations of dihydroxyacetone a slight inhibition was noted. No loss of activity of the immobilized preparation was observed after storage for 68 days at +4°C. Investigation of the operational stability revealed a half-life of 5 days. Studies of the influence of particle size and cell densities as well as that of oxygen concentration revealed that the oxygen supply was the rate limiting step.

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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Applied Microbiology and Biotechnology
volume
22
issue
1
pages
7 pages
publisher
Springer
external identifiers
  • scopus:0021979025
ISSN
0175-7598
DOI
10.1007/BF00252148
language
English
LU publication?
yes
id
ca605beb-d797-4e14-bb4f-3b141ae21be9
date added to LUP
2019-06-22 19:03:09
date last changed
2021-10-10 03:27:41
@article{ca605beb-d797-4e14-bb4f-3b141ae21be9,
  abstract     = {{<p>Gluconobacter oxydans cells were immobilized in calcium alginate and the preparation was used for the oxidation of glycerol to dihydroxyacetone. The characterization was done according to the guidelines given by the Working Party on Immobilized Biocatalysts of the European Federation of Biotechnology. The pH optimum of the preparation was found to be 5.0 and the temperature optimum was 40°C. However, the operational stability was better at 30°C. The glycerol concentration required to obtain half the maximal reaction rate was about 5 mM for both immobilized and free cells. At low concentrations of glycerol and high concentrations of dihydroxyacetone a slight inhibition was noted. No loss of activity of the immobilized preparation was observed after storage for 68 days at +4°C. Investigation of the operational stability revealed a half-life of 5 days. Studies of the influence of particle size and cell densities as well as that of oxygen concentration revealed that the oxygen supply was the rate limiting step.</p>}},
  author       = {{Adlercreutz, Patrick and Holst, Olle and Mattiasson, Bo}},
  issn         = {{0175-7598}},
  language     = {{eng}},
  month        = {{05}},
  number       = {{1}},
  pages        = {{1--7}},
  publisher    = {{Springer}},
  series       = {{Applied Microbiology and Biotechnology}},
  title        = {{Characterization of Gluconobacter oxydans immobilized in calcium alginate}},
  url          = {{http://dx.doi.org/10.1007/BF00252148}},
  doi          = {{10.1007/BF00252148}},
  volume       = {{22}},
  year         = {{1985}},
}