Characterization of Gluconobacter oxydans immobilized in calcium alginate
(1985) In Applied Microbiology and Biotechnology 22(1). p.1-7- Abstract
Gluconobacter oxydans cells were immobilized in calcium alginate and the preparation was used for the oxidation of glycerol to dihydroxyacetone. The characterization was done according to the guidelines given by the Working Party on Immobilized Biocatalysts of the European Federation of Biotechnology. The pH optimum of the preparation was found to be 5.0 and the temperature optimum was 40°C. However, the operational stability was better at 30°C. The glycerol concentration required to obtain half the maximal reaction rate was about 5 mM for both immobilized and free cells. At low concentrations of glycerol and high concentrations of dihydroxyacetone a slight inhibition was noted. No loss of activity of the immobilized preparation was... (More)
Gluconobacter oxydans cells were immobilized in calcium alginate and the preparation was used for the oxidation of glycerol to dihydroxyacetone. The characterization was done according to the guidelines given by the Working Party on Immobilized Biocatalysts of the European Federation of Biotechnology. The pH optimum of the preparation was found to be 5.0 and the temperature optimum was 40°C. However, the operational stability was better at 30°C. The glycerol concentration required to obtain half the maximal reaction rate was about 5 mM for both immobilized and free cells. At low concentrations of glycerol and high concentrations of dihydroxyacetone a slight inhibition was noted. No loss of activity of the immobilized preparation was observed after storage for 68 days at +4°C. Investigation of the operational stability revealed a half-life of 5 days. Studies of the influence of particle size and cell densities as well as that of oxygen concentration revealed that the oxygen supply was the rate limiting step.
(Less)
- author
- Adlercreutz, Patrick LU ; Holst, Olle LU and Mattiasson, Bo LU
- organization
- publishing date
- 1985-05-01
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Applied Microbiology and Biotechnology
- volume
- 22
- issue
- 1
- pages
- 7 pages
- publisher
- Springer
- external identifiers
-
- scopus:0021979025
- ISSN
- 0175-7598
- DOI
- 10.1007/BF00252148
- language
- English
- LU publication?
- yes
- id
- ca605beb-d797-4e14-bb4f-3b141ae21be9
- date added to LUP
- 2019-06-22 19:03:09
- date last changed
- 2021-10-10 03:27:41
@article{ca605beb-d797-4e14-bb4f-3b141ae21be9, abstract = {{<p>Gluconobacter oxydans cells were immobilized in calcium alginate and the preparation was used for the oxidation of glycerol to dihydroxyacetone. The characterization was done according to the guidelines given by the Working Party on Immobilized Biocatalysts of the European Federation of Biotechnology. The pH optimum of the preparation was found to be 5.0 and the temperature optimum was 40°C. However, the operational stability was better at 30°C. The glycerol concentration required to obtain half the maximal reaction rate was about 5 mM for both immobilized and free cells. At low concentrations of glycerol and high concentrations of dihydroxyacetone a slight inhibition was noted. No loss of activity of the immobilized preparation was observed after storage for 68 days at +4°C. Investigation of the operational stability revealed a half-life of 5 days. Studies of the influence of particle size and cell densities as well as that of oxygen concentration revealed that the oxygen supply was the rate limiting step.</p>}}, author = {{Adlercreutz, Patrick and Holst, Olle and Mattiasson, Bo}}, issn = {{0175-7598}}, language = {{eng}}, month = {{05}}, number = {{1}}, pages = {{1--7}}, publisher = {{Springer}}, series = {{Applied Microbiology and Biotechnology}}, title = {{Characterization of Gluconobacter oxydans immobilized in calcium alginate}}, url = {{http://dx.doi.org/10.1007/BF00252148}}, doi = {{10.1007/BF00252148}}, volume = {{22}}, year = {{1985}}, }