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Liquid chromatographic determination of diltiazem and its metabolites using trans isomers as internal standards, with dynamic modification of the solid phase by addition of an amine to the mobile phase

Höglund, P LU and Nilsson, L G (1987) In Journal of Chromatography 414(1). p.20-109
Abstract

A selective and sensitive reversed-phase high-performance liquid chromatographic method for simultaneous analysis of diltiazem and its five metabolites known to occur in man is presented. Different C18 columns are compared. The influences of organic co-solvent, pH, ionic strength and the addition of various amines are studied. The use of trans-diltiazem as internal standard is advocated. UV detection at 237 nm is used for plasma analyses. The possibilities of electrochemical detection are also discussed. Before solvent extraction, plasma samples are saturated with sodium chloride and made alkaline. Triethylamine is also added. The use of the method in pharmacokinetic applications is exemplified.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Amines, Biotransformation, Chromatography, High Pressure Liquid, Diltiazem, Humans, Kinetics, Spectrophotometry, Ultraviolet, Stereoisomerism, Journal Article
in
Journal of Chromatography
volume
414
issue
1
pages
20 - 109
publisher
Elsevier
external identifiers
  • scopus:0023153169
ISSN
0021-9673
language
English
LU publication?
yes
id
cad77ac3-3fae-4a96-88d8-21fa843b4922
date added to LUP
2017-09-14 23:51:21
date last changed
2017-09-18 16:26:45
@article{cad77ac3-3fae-4a96-88d8-21fa843b4922,
  abstract     = {<p>A selective and sensitive reversed-phase high-performance liquid chromatographic method for simultaneous analysis of diltiazem and its five metabolites known to occur in man is presented. Different C18 columns are compared. The influences of organic co-solvent, pH, ionic strength and the addition of various amines are studied. The use of trans-diltiazem as internal standard is advocated. UV detection at 237 nm is used for plasma analyses. The possibilities of electrochemical detection are also discussed. Before solvent extraction, plasma samples are saturated with sodium chloride and made alkaline. Triethylamine is also added. The use of the method in pharmacokinetic applications is exemplified.</p>},
  author       = {Höglund, P and Nilsson, L G},
  issn         = {0021-9673},
  keyword      = {Amines,Biotransformation,Chromatography, High Pressure Liquid,Diltiazem,Humans,Kinetics,Spectrophotometry, Ultraviolet,Stereoisomerism,Journal Article},
  language     = {eng},
  month        = {02},
  number       = {1},
  pages        = {20--109},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography},
  title        = {Liquid chromatographic determination of diltiazem and its metabolites using trans isomers as internal standards, with dynamic modification of the solid phase by addition of an amine to the mobile phase},
  volume       = {414},
  year         = {1987},
}