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A reduced population of CD103+CD11b+ dendritic cells has a limited impact on oral Salmonella infection

Fernández-Santoscoy, María ; Wenzel, Ulf Alexander ; Persson, Emma LU ; Yrlid, Ulf LU ; Agace, William LU and Wick, Mary Jo LU (2016) In Immunology Letters 176. p.72-80
Abstract

CD103+CD11b+ dendritic cells (DC) are the major migratory DC subset in the small intestine lamina propria (siLP) and their survival is dependent on the transcription factor interferon regulatory factor 4 (IRF4). Mice with a DC-specific deletion of irf4 (CD11c-cre.Irf4 mice) have reduced mucosal CD103+CD11b+ DC and altered T cell differentiation to protein antigen. The influence of CD103+CD11b+ DC on oral infection with the gastrointestinal pathogen Salmonella, however, is poorly understood and is investigated here. We show that, despite being infected with Salmonella, CD11c-cre.Irf4 mice (called Cre+ mice) conserve the reduction in... (More)

CD103+CD11b+ dendritic cells (DC) are the major migratory DC subset in the small intestine lamina propria (siLP) and their survival is dependent on the transcription factor interferon regulatory factor 4 (IRF4). Mice with a DC-specific deletion of irf4 (CD11c-cre.Irf4 mice) have reduced mucosal CD103+CD11b+ DC and altered T cell differentiation to protein antigen. The influence of CD103+CD11b+ DC on oral infection with the gastrointestinal pathogen Salmonella, however, is poorly understood and is investigated here. We show that, despite being infected with Salmonella, CD11c-cre.Irf4 mice (called Cre+ mice) conserve the reduction in CD103+CD11b+ DC observed in naive Cre+ mice, particularly in the mesenteric lymph nodes (MLN) but also in the siLP at day 3 post infection. Moreover, Salmonella-infected Cre+ mice have a similar bacterial burden in intestinal tissues (siLP, MLN and Peyer's patches) as well as the spleen compared to infected Cre- controls. The T cell compartment, including the frequency of IFN-γ and IL-17-producing T cells, is not altered in intestinal tissues of Salmonella-infected Cre+ mice relative to infected Cre- controls. In addition, no difference between infected Cre+ and Cre- mice was observed in either the concentration of IL-6 or IL-17 in whole tissue lysates of siLP, MLN or Peyer's patches or in the serum concentration of Salmonella-specific IgG and IgM. Overall the data suggest that the reduction of CD103+CD11b+ DC in Cre+ mice has little if any impact on Salmonella burden in infected tissues or eliciting effector functions important in host survival at later stages of the infection.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
CD103, Dendritic cells, Infection, IRF4, Mesenteric lymph node, Salmonella
in
Immunology Letters
volume
176
pages
9 pages
publisher
Elsevier
external identifiers
  • pmid:27262930
  • wos:000381324400010
  • scopus:84974715605
ISSN
0165-2478
DOI
10.1016/j.imlet.2016.05.012
language
English
LU publication?
yes
id
cb0d97d8-cedb-4cd7-bb79-a737eaf90837
date added to LUP
2016-07-06 15:01:49
date last changed
2025-01-12 08:32:54
@article{cb0d97d8-cedb-4cd7-bb79-a737eaf90837,
  abstract     = {{<p>CD103<sup>+</sup>CD11b<sup>+</sup> dendritic cells (DC) are the major migratory DC subset in the small intestine lamina propria (siLP) and their survival is dependent on the transcription factor interferon regulatory factor 4 (IRF4). Mice with a DC-specific deletion of irf4 (CD11c-cre.Irf4 mice) have reduced mucosal CD103<sup>+</sup>CD11b<sup>+</sup> DC and altered T cell differentiation to protein antigen. The influence of CD103<sup>+</sup>CD11b<sup>+</sup> DC on oral infection with the gastrointestinal pathogen Salmonella, however, is poorly understood and is investigated here. We show that, despite being infected with Salmonella, CD11c-cre.Irf4 mice (called Cre<sup>+</sup> mice) conserve the reduction in CD103<sup>+</sup>CD11b<sup>+</sup> DC observed in naive Cre<sup>+</sup> mice, particularly in the mesenteric lymph nodes (MLN) but also in the siLP at day 3 post infection. Moreover, Salmonella-infected Cre<sup>+</sup> mice have a similar bacterial burden in intestinal tissues (siLP, MLN and Peyer's patches) as well as the spleen compared to infected Cre<sup>-</sup> controls. The T cell compartment, including the frequency of IFN-γ and IL-17-producing T cells, is not altered in intestinal tissues of Salmonella-infected Cre<sup>+</sup> mice relative to infected Cre<sup>-</sup> controls. In addition, no difference between infected Cre<sup>+</sup> and Cre<sup>-</sup> mice was observed in either the concentration of IL-6 or IL-17 in whole tissue lysates of siLP, MLN or Peyer's patches or in the serum concentration of Salmonella-specific IgG and IgM. Overall the data suggest that the reduction of CD103<sup>+</sup>CD11b<sup>+</sup> DC in Cre<sup>+</sup> mice has little if any impact on Salmonella burden in infected tissues or eliciting effector functions important in host survival at later stages of the infection.</p>}},
  author       = {{Fernández-Santoscoy, María and Wenzel, Ulf Alexander and Persson, Emma and Yrlid, Ulf and Agace, William and Wick, Mary Jo}},
  issn         = {{0165-2478}},
  keywords     = {{CD103; Dendritic cells; Infection; IRF4; Mesenteric lymph node; Salmonella}},
  language     = {{eng}},
  month        = {{08}},
  pages        = {{72--80}},
  publisher    = {{Elsevier}},
  series       = {{Immunology Letters}},
  title        = {{A reduced population of CD103<sup>+</sup>CD11b<sup>+</sup> dendritic cells has a limited impact on oral Salmonella infection}},
  url          = {{http://dx.doi.org/10.1016/j.imlet.2016.05.012}},
  doi          = {{10.1016/j.imlet.2016.05.012}},
  volume       = {{176}},
  year         = {{2016}},
}