Fully automated microchip system for the detection of quantal exocytosis from single and small ensembles of cells
(2008) In Lab on a Chip 2(8). p.323-329- Abstract
- A lab-on-a-chip device that enables positioning of single or small ensembles of cells on an aperture in close proximity to a mercaptopropionic acid (MPA) modified sensing electrode has been developed and characterized. The microchip was used for the detection of Ca(2+)-dependent quantal catecholamine exocytosis from single as well as small assemblies of rat pheochromocytoma (PC12) cells. The frequency of events increased considerably upon depolarization of the PC12 cell membrane using a high extracelluar concentration of potassium. The number of recorded events could be correlated with the number of cells immobilized on the electrode. Quantal characteristics, such as the number of released molecules per recorded event, are equivalent to... (More)
- A lab-on-a-chip device that enables positioning of single or small ensembles of cells on an aperture in close proximity to a mercaptopropionic acid (MPA) modified sensing electrode has been developed and characterized. The microchip was used for the detection of Ca(2+)-dependent quantal catecholamine exocytosis from single as well as small assemblies of rat pheochromocytoma (PC12) cells. The frequency of events increased considerably upon depolarization of the PC12 cell membrane using a high extracelluar concentration of potassium. The number of recorded events could be correlated with the number of cells immobilized on the electrode. Quantal characteristics, such as the number of released molecules per recorded event, are equivalent to data obtained using conventional carbon fiber microelectrodes. The detection sensitivity of the device allows for the detection of less than 10 000 dopamine molecules in a quantal release. The distribution of peak rise-time and full width at half maximum was constant during measurement periods of several minutes demonstrating the stability of the MPA modified surface. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1042528
- author
- Spegel, Christer LU ; Heiskanen, Arto LU ; Pedersen, Simon ; Emnéus, Jenny LU ; Ruzgas, Tautgirdas LU and Taboryski, Rafael
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Lab on a Chip
- volume
- 2
- issue
- 8
- pages
- 323 - 329
- publisher
- Royal Society of Chemistry
- external identifiers
-
- wos:000252758100017
- scopus:38849090885
- ISSN
- 1473-0189
- DOI
- 10.1039/b715107a
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- cb665ba9-3d40-4ef9-a1f7-336a2a985dac (old id 1042528)
- date added to LUP
- 2016-04-01 12:14:20
- date last changed
- 2022-03-13 07:12:26
@article{cb665ba9-3d40-4ef9-a1f7-336a2a985dac,
abstract = {{A lab-on-a-chip device that enables positioning of single or small ensembles of cells on an aperture in close proximity to a mercaptopropionic acid (MPA) modified sensing electrode has been developed and characterized. The microchip was used for the detection of Ca(2+)-dependent quantal catecholamine exocytosis from single as well as small assemblies of rat pheochromocytoma (PC12) cells. The frequency of events increased considerably upon depolarization of the PC12 cell membrane using a high extracelluar concentration of potassium. The number of recorded events could be correlated with the number of cells immobilized on the electrode. Quantal characteristics, such as the number of released molecules per recorded event, are equivalent to data obtained using conventional carbon fiber microelectrodes. The detection sensitivity of the device allows for the detection of less than 10 000 dopamine molecules in a quantal release. The distribution of peak rise-time and full width at half maximum was constant during measurement periods of several minutes demonstrating the stability of the MPA modified surface.}},
author = {{Spegel, Christer and Heiskanen, Arto and Pedersen, Simon and Emnéus, Jenny and Ruzgas, Tautgirdas and Taboryski, Rafael}},
issn = {{1473-0189}},
language = {{eng}},
number = {{8}},
pages = {{323--329}},
publisher = {{Royal Society of Chemistry}},
series = {{Lab on a Chip}},
title = {{Fully automated microchip system for the detection of quantal exocytosis from single and small ensembles of cells}},
url = {{http://dx.doi.org/10.1039/b715107a}},
doi = {{10.1039/b715107a}},
volume = {{2}},
year = {{2008}},
}