The antimicrobial peptide LL-37 facilitates the formation of neutrophil extracellular traps
(2014) In The Biochemical journal 464(1). p.3-11- Abstract
NETs (neutrophil extracellular traps) have been described as a fundamental innate immune defence mechanism. During formation of NETs, the nuclear membrane is disrupted by an as-yet unknown mechanism. In the present study we investigated the role of human cathelicidin LL-37 in nuclear membrane disruption and formation of NETs. Immunofluorescence microscopy revealed that 5 μM LL-37 significantly facilitated NET formation by primary human blood-derived neutrophils alone, in the presence of the classical chemical NET inducer PMA or in the presence of Staphylococcus aureus. Parallel assays with a random LL-37 fragment library indicated that the NET induction is mediated by the hydrophobic character of the peptide. The trans-localization of... (More)
NETs (neutrophil extracellular traps) have been described as a fundamental innate immune defence mechanism. During formation of NETs, the nuclear membrane is disrupted by an as-yet unknown mechanism. In the present study we investigated the role of human cathelicidin LL-37 in nuclear membrane disruption and formation of NETs. Immunofluorescence microscopy revealed that 5 μM LL-37 significantly facilitated NET formation by primary human blood-derived neutrophils alone, in the presence of the classical chemical NET inducer PMA or in the presence of Staphylococcus aureus. Parallel assays with a random LL-37 fragment library indicated that the NET induction is mediated by the hydrophobic character of the peptide. The trans-localization of LL-37 towards the nucleus and the disruption of the nuclear membrane were visualized using confocal fluorescence microscopy. In conclusion, the present study demonstrates a novel role for LL-37 in the formation of NETs.
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- author
- Neumann, Ariane LU ; Berends, Evelien T. M. ; Nerlich, Andreas ; Molhoek, E. Margo ; Gallo, Richard L. ; Meerloo, Timo ; Nizet, Victor ; Naim, Hassan Y. and von Köckritz-Blickwede, Maren
- publishing date
- 2014-11-15
- type
- Contribution to journal
- publication status
- published
- keywords
- Amino Acid Sequence, Animals, Cathelicidins, Extracellular Traps, Humans, Male, Mice, Mice, Inbred C57BL, Molecular Sequence Data, Neutrophils, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't
- in
- The Biochemical journal
- volume
- 464
- issue
- 1
- pages
- 9 pages
- publisher
- Portland Press
- external identifiers
-
- scopus:84908453654
- pmid:25181554
- ISSN
- 0264-6021
- DOI
- 10.1042/BJ20140778
- language
- English
- LU publication?
- no
- id
- cbd66a6f-2967-4537-a20c-aeac98c2631d
- date added to LUP
- 2017-09-19 12:16:07
- date last changed
- 2024-02-13 04:21:26
@article{cbd66a6f-2967-4537-a20c-aeac98c2631d,
abstract = {{<p>NETs (neutrophil extracellular traps) have been described as a fundamental innate immune defence mechanism. During formation of NETs, the nuclear membrane is disrupted by an as-yet unknown mechanism. In the present study we investigated the role of human cathelicidin LL-37 in nuclear membrane disruption and formation of NETs. Immunofluorescence microscopy revealed that 5 μM LL-37 significantly facilitated NET formation by primary human blood-derived neutrophils alone, in the presence of the classical chemical NET inducer PMA or in the presence of Staphylococcus aureus. Parallel assays with a random LL-37 fragment library indicated that the NET induction is mediated by the hydrophobic character of the peptide. The trans-localization of LL-37 towards the nucleus and the disruption of the nuclear membrane were visualized using confocal fluorescence microscopy. In conclusion, the present study demonstrates a novel role for LL-37 in the formation of NETs.</p>}},
author = {{Neumann, Ariane and Berends, Evelien T. M. and Nerlich, Andreas and Molhoek, E. Margo and Gallo, Richard L. and Meerloo, Timo and Nizet, Victor and Naim, Hassan Y. and von Köckritz-Blickwede, Maren}},
issn = {{0264-6021}},
keywords = {{Amino Acid Sequence; Animals; Cathelicidins; Extracellular Traps; Humans; Male; Mice; Mice, Inbred C57BL; Molecular Sequence Data; Neutrophils; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't}},
language = {{eng}},
month = {{11}},
number = {{1}},
pages = {{3--11}},
publisher = {{Portland Press}},
series = {{The Biochemical journal}},
title = {{The antimicrobial peptide LL-37 facilitates the formation of neutrophil extracellular traps}},
url = {{http://dx.doi.org/10.1042/BJ20140778}},
doi = {{10.1042/BJ20140778}},
volume = {{464}},
year = {{2014}},
}