Advanced

Neutron spectroscopy on protein solutions employing backscattering with an increased energy range

Beck, Christian; Appel, Markus; Grimaldo, Marco; Roosen-Runge, Felix LU ; Zhang, Fajun LU ; Frick, Bernhard; Schreiber, Frank and Seydel, Tilo (2019) In Physica B: Condensed Matter 562. p.31-35
Abstract

Novel cold neutron backscattering spectrometers contribute substantially to the understanding of the diffusive dynamics of proteins in dense aqueous suspensions. Such suspensions are fundamentally interesting for instance in terms of the so-called macromolecular crowding, protein cluster formation, gelation, and self-assembly. Notably, backscattering spectrometers with the highest flux can simultaneously access the center-of-mass diffusion of the proteins and the superimposed internal molecular diffusive motions. The nearly complete absence of protein-protein collisions on the accessible nanosecond observation time scale even in dense protein suspensions implies that neutron backscattering accesses the so-called short-time limit for the... (More)

Novel cold neutron backscattering spectrometers contribute substantially to the understanding of the diffusive dynamics of proteins in dense aqueous suspensions. Such suspensions are fundamentally interesting for instance in terms of the so-called macromolecular crowding, protein cluster formation, gelation, and self-assembly. Notably, backscattering spectrometers with the highest flux can simultaneously access the center-of-mass diffusion of the proteins and the superimposed internal molecular diffusive motions. The nearly complete absence of protein-protein collisions on the accessible nanosecond observation time scale even in dense protein suspensions implies that neutron backscattering accesses the so-called short-time limit for the center-of-mass diffusion. This limit is particularly interesting in terms of a theoretical understanding by concepts from colloid physics. Here we briefly review recent progress in studying protein dynamics achieved with the latest generation of backscattering spectrometers. We illustrate this progress by the first data from a protein solution using the backscattering-and-time-of-flight option BATS on IN16B at the ILL and we outline future perspectives.

(Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Aqueous protein solutions, Cold neutron backscattering, Self-diffusion
in
Physica B: Condensed Matter
volume
562
pages
5 pages
publisher
Elsevier
external identifiers
  • scopus:85062953084
ISSN
0921-4526
DOI
10.1016/j.physb.2018.11.058
language
English
LU publication?
yes
id
cbefabb4-6060-4dcf-bfbf-a2d2401f3e90
date added to LUP
2019-03-25 12:24:42
date last changed
2019-04-23 04:47:20
@article{cbefabb4-6060-4dcf-bfbf-a2d2401f3e90,
  abstract     = {<p>Novel cold neutron backscattering spectrometers contribute substantially to the understanding of the diffusive dynamics of proteins in dense aqueous suspensions. Such suspensions are fundamentally interesting for instance in terms of the so-called macromolecular crowding, protein cluster formation, gelation, and self-assembly. Notably, backscattering spectrometers with the highest flux can simultaneously access the center-of-mass diffusion of the proteins and the superimposed internal molecular diffusive motions. The nearly complete absence of protein-protein collisions on the accessible nanosecond observation time scale even in dense protein suspensions implies that neutron backscattering accesses the so-called short-time limit for the center-of-mass diffusion. This limit is particularly interesting in terms of a theoretical understanding by concepts from colloid physics. Here we briefly review recent progress in studying protein dynamics achieved with the latest generation of backscattering spectrometers. We illustrate this progress by the first data from a protein solution using the backscattering-and-time-of-flight option BATS on IN16B at the ILL and we outline future perspectives.</p>},
  author       = {Beck, Christian and Appel, Markus and Grimaldo, Marco and Roosen-Runge, Felix and Zhang, Fajun and Frick, Bernhard and Schreiber, Frank and Seydel, Tilo},
  issn         = {0921-4526},
  keyword      = {Aqueous protein solutions,Cold neutron backscattering,Self-diffusion},
  language     = {eng},
  pages        = {31--35},
  publisher    = {Elsevier},
  series       = {Physica B: Condensed Matter},
  title        = {Neutron spectroscopy on protein solutions employing backscattering with an increased energy range},
  url          = {http://dx.doi.org/10.1016/j.physb.2018.11.058},
  volume       = {562},
  year         = {2019},
}