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Massive intein content in Anaeramoeba reveals aspects of intein mobility in eukaryotes

Gallot-Lavallée, Lucie ; Jerlström-Hultqvist, Jon ; Zegarra-Vidarte, Paula ; Salas-Leiva, Dayana E ; Stairs, Courtney W LU orcid ; Čepička, Ivan ; Roger, Andrew J and Archibald, John M (2023) In Proceedings of the National Academy of Sciences of the United States of America 120(49).
Abstract

Inteins are self-splicing protein elements found in viruses and all three domains of life. How the DNA encoding these selfish elements spreads within and between genomes is poorly understood, particularly in eukaryotes where inteins are scarce. Here, we show that the nuclear genomes of three strains of
Anaeramoeba encode between 45 and 103 inteins, in stark contrast to four found in the most intein-rich eukaryotic genome described previously. The
Anaeramoeba inteins reside in a wide range of proteins, only some of which correspond to intein-containing proteins in other eukaryotes, prokaryotes, and viruses. Our data also suggest that viruses have contributed to the spread of inteins in
Anaeramoeba and the colonization... (More)

Inteins are self-splicing protein elements found in viruses and all three domains of life. How the DNA encoding these selfish elements spreads within and between genomes is poorly understood, particularly in eukaryotes where inteins are scarce. Here, we show that the nuclear genomes of three strains of
Anaeramoeba encode between 45 and 103 inteins, in stark contrast to four found in the most intein-rich eukaryotic genome described previously. The
Anaeramoeba inteins reside in a wide range of proteins, only some of which correspond to intein-containing proteins in other eukaryotes, prokaryotes, and viruses. Our data also suggest that viruses have contributed to the spread of inteins in
Anaeramoeba and the colonization of new alleles. The persistence of
Anaeramoeba inteins might be partly explained by intragenomic movement of intein-encoding regions from gene to gene. Our intein dataset greatly expands the spectrum of intein-containing proteins and provides insights into the evolution of inteins in eukaryotes.

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author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Inteins/genetics, Protein Splicing, Eukaryota/genetics, Proteins/genetics, Genome
in
Proceedings of the National Academy of Sciences of the United States of America
volume
120
issue
49
article number
e2306381120
pages
9 pages
publisher
National Academy of Sciences
external identifiers
  • scopus:85178212433
  • pmid:38019867
ISSN
1091-6490
DOI
10.1073/pnas.2306381120
language
English
LU publication?
yes
id
cc2ae07f-89b0-4394-9028-ce33eae50394
date added to LUP
2023-12-03 10:27:09
date last changed
2024-04-21 21:35:07
@article{cc2ae07f-89b0-4394-9028-ce33eae50394,
  abstract     = {{<p>Inteins are self-splicing protein elements found in viruses and all three domains of life. How the DNA encoding these selfish elements spreads within and between genomes is poorly understood, particularly in eukaryotes where inteins are scarce. Here, we show that the nuclear genomes of three strains of <br>
 Anaeramoeba encode between 45 and 103 inteins, in stark contrast to four found in the most intein-rich eukaryotic genome described previously. The <br>
 Anaeramoeba inteins reside in a wide range of proteins, only some of which correspond to intein-containing proteins in other eukaryotes, prokaryotes, and viruses. Our data also suggest that viruses have contributed to the spread of inteins in <br>
 Anaeramoeba and the colonization of new alleles. The persistence of <br>
 Anaeramoeba inteins might be partly explained by intragenomic movement of intein-encoding regions from gene to gene. Our intein dataset greatly expands the spectrum of intein-containing proteins and provides insights into the evolution of inteins in eukaryotes.<br>
 </p>}},
  author       = {{Gallot-Lavallée, Lucie and Jerlström-Hultqvist, Jon and Zegarra-Vidarte, Paula and Salas-Leiva, Dayana E and Stairs, Courtney W and Čepička, Ivan and Roger, Andrew J and Archibald, John M}},
  issn         = {{1091-6490}},
  keywords     = {{Inteins/genetics; Protein Splicing; Eukaryota/genetics; Proteins/genetics; Genome}},
  language     = {{eng}},
  month        = {{12}},
  number       = {{49}},
  publisher    = {{National Academy of Sciences}},
  series       = {{Proceedings of the National Academy of Sciences of the United States of America}},
  title        = {{Massive intein content in Anaeramoeba reveals aspects of intein mobility in eukaryotes}},
  url          = {{http://dx.doi.org/10.1073/pnas.2306381120}},
  doi          = {{10.1073/pnas.2306381120}},
  volume       = {{120}},
  year         = {{2023}},
}