Massive intein content in Anaeramoeba reveals aspects of intein mobility in eukaryotes
(2023) In Proceedings of the National Academy of Sciences of the United States of America 120(49).- Abstract
Inteins are self-splicing protein elements found in viruses and all three domains of life. How the DNA encoding these selfish elements spreads within and between genomes is poorly understood, particularly in eukaryotes where inteins are scarce. Here, we show that the nuclear genomes of three strains of
Anaeramoeba encode between 45 and 103 inteins, in stark contrast to four found in the most intein-rich eukaryotic genome described previously. The
Anaeramoeba inteins reside in a wide range of proteins, only some of which correspond to intein-containing proteins in other eukaryotes, prokaryotes, and viruses. Our data also suggest that viruses have contributed to the spread of inteins in
Anaeramoeba and the colonization... (More)Inteins are self-splicing protein elements found in viruses and all three domains of life. How the DNA encoding these selfish elements spreads within and between genomes is poorly understood, particularly in eukaryotes where inteins are scarce. Here, we show that the nuclear genomes of three strains of
(Less)
Anaeramoeba encode between 45 and 103 inteins, in stark contrast to four found in the most intein-rich eukaryotic genome described previously. The
Anaeramoeba inteins reside in a wide range of proteins, only some of which correspond to intein-containing proteins in other eukaryotes, prokaryotes, and viruses. Our data also suggest that viruses have contributed to the spread of inteins in
Anaeramoeba and the colonization of new alleles. The persistence of
Anaeramoeba inteins might be partly explained by intragenomic movement of intein-encoding regions from gene to gene. Our intein dataset greatly expands the spectrum of intein-containing proteins and provides insights into the evolution of inteins in eukaryotes.
- author
- Gallot-Lavallée, Lucie ; Jerlström-Hultqvist, Jon ; Zegarra-Vidarte, Paula ; Salas-Leiva, Dayana E ; Stairs, Courtney W LU ; Čepička, Ivan ; Roger, Andrew J and Archibald, John M
- organization
- publishing date
- 2023-12-05
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Inteins/genetics, Protein Splicing, Eukaryota/genetics, Proteins/genetics, Genome
- in
- Proceedings of the National Academy of Sciences of the United States of America
- volume
- 120
- issue
- 49
- article number
- e2306381120
- pages
- 9 pages
- publisher
- National Academy of Sciences
- external identifiers
-
- pmid:38019867
- scopus:85178212433
- ISSN
- 1091-6490
- DOI
- 10.1073/pnas.2306381120
- language
- English
- LU publication?
- yes
- id
- cc2ae07f-89b0-4394-9028-ce33eae50394
- date added to LUP
- 2023-12-03 10:27:09
- date last changed
- 2024-06-17 02:28:24
@article{cc2ae07f-89b0-4394-9028-ce33eae50394, abstract = {{<p>Inteins are self-splicing protein elements found in viruses and all three domains of life. How the DNA encoding these selfish elements spreads within and between genomes is poorly understood, particularly in eukaryotes where inteins are scarce. Here, we show that the nuclear genomes of three strains of <br> Anaeramoeba encode between 45 and 103 inteins, in stark contrast to four found in the most intein-rich eukaryotic genome described previously. The <br> Anaeramoeba inteins reside in a wide range of proteins, only some of which correspond to intein-containing proteins in other eukaryotes, prokaryotes, and viruses. Our data also suggest that viruses have contributed to the spread of inteins in <br> Anaeramoeba and the colonization of new alleles. The persistence of <br> Anaeramoeba inteins might be partly explained by intragenomic movement of intein-encoding regions from gene to gene. Our intein dataset greatly expands the spectrum of intein-containing proteins and provides insights into the evolution of inteins in eukaryotes.<br> </p>}}, author = {{Gallot-Lavallée, Lucie and Jerlström-Hultqvist, Jon and Zegarra-Vidarte, Paula and Salas-Leiva, Dayana E and Stairs, Courtney W and Čepička, Ivan and Roger, Andrew J and Archibald, John M}}, issn = {{1091-6490}}, keywords = {{Inteins/genetics; Protein Splicing; Eukaryota/genetics; Proteins/genetics; Genome}}, language = {{eng}}, month = {{12}}, number = {{49}}, publisher = {{National Academy of Sciences}}, series = {{Proceedings of the National Academy of Sciences of the United States of America}}, title = {{Massive intein content in Anaeramoeba reveals aspects of intein mobility in eukaryotes}}, url = {{http://dx.doi.org/10.1073/pnas.2306381120}}, doi = {{10.1073/pnas.2306381120}}, volume = {{120}}, year = {{2023}}, }