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L-Arabinose metabolism in Candida arabinofermentans PYCC 5603(T) and Pichia guilliermondii PYCC 3012: influence of sugar and oxygen on product formation

Fonseca, Cesar ; Spencer-Martins, Isabel and Hahn-Hägerdal, Bärbel LU (2007) In Applied Microbiology and Biotechnology 75(2). p.303-310
Abstract
L-Arabinose utilization by the yeasts Candida arabinofermentans PYCC 5603(T) and Pichia guilliermondii PYCC 3012 was investigated in aerobic batch cultures and compared, under similar conditions, to D-glucose and D-xylose metabolism. At high aeration levels, only biomass was formed from all the three sugars. When oxygen became limited, ethanol was produced from D-glucose, demonstrating a fermentative pathway in these yeasts. However, pentoses were essentially respired and, under oxygen limitation, the respective polyols accumulated-arabitol from L-arabinose and xylitol from D-xylose. Different L-arabinose concentrations and oxygen conditions were tested to better understand L-arabinose metabolism. P. guilliermondii PYCC 3012 excreted... (More)
L-Arabinose utilization by the yeasts Candida arabinofermentans PYCC 5603(T) and Pichia guilliermondii PYCC 3012 was investigated in aerobic batch cultures and compared, under similar conditions, to D-glucose and D-xylose metabolism. At high aeration levels, only biomass was formed from all the three sugars. When oxygen became limited, ethanol was produced from D-glucose, demonstrating a fermentative pathway in these yeasts. However, pentoses were essentially respired and, under oxygen limitation, the respective polyols accumulated-arabitol from L-arabinose and xylitol from D-xylose. Different L-arabinose concentrations and oxygen conditions were tested to better understand L-arabinose metabolism. P. guilliermondii PYCC 3012 excreted considerably more arabitol from L-arabinose (and also xylitol from D-xylose) than C arabinofermentans PYCC 5603(T). In contrast to the latter, P guilliermondii PYCC 3012 did not produce any traces of ethanol in complex L-arabinose (80 g/l) medium under oxygen-limited conditions. Neither sustained growth nor active metabolism was observed under anaerobiosis. This study demonstrates, for the first time, the oxygen dependence of metabolite and product formation in L-arabinose-assimilating yeasts. (Less)
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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Hemicellulose, yeast, fermentation conditions, L-Arabinose, pentose, Candida arabinofermentans, Pichia guilliermondii
in
Applied Microbiology and Biotechnology
volume
75
issue
2
pages
303 - 310
publisher
Springer
external identifiers
  • wos:000246842500008
  • scopus:33947254105
ISSN
1432-0614
DOI
10.1007/s00253-006-0830-7
language
English
LU publication?
yes
id
cc837718-f65a-4b5d-878f-d41bd1d8c745 (old id 657593)
date added to LUP
2016-04-01 16:24:53
date last changed
2022-02-12 22:02:44
@article{cc837718-f65a-4b5d-878f-d41bd1d8c745,
  abstract     = {{L-Arabinose utilization by the yeasts Candida arabinofermentans PYCC 5603(T) and Pichia guilliermondii PYCC 3012 was investigated in aerobic batch cultures and compared, under similar conditions, to D-glucose and D-xylose metabolism. At high aeration levels, only biomass was formed from all the three sugars. When oxygen became limited, ethanol was produced from D-glucose, demonstrating a fermentative pathway in these yeasts. However, pentoses were essentially respired and, under oxygen limitation, the respective polyols accumulated-arabitol from L-arabinose and xylitol from D-xylose. Different L-arabinose concentrations and oxygen conditions were tested to better understand L-arabinose metabolism. P. guilliermondii PYCC 3012 excreted considerably more arabitol from L-arabinose (and also xylitol from D-xylose) than C arabinofermentans PYCC 5603(T). In contrast to the latter, P guilliermondii PYCC 3012 did not produce any traces of ethanol in complex L-arabinose (80 g/l) medium under oxygen-limited conditions. Neither sustained growth nor active metabolism was observed under anaerobiosis. This study demonstrates, for the first time, the oxygen dependence of metabolite and product formation in L-arabinose-assimilating yeasts.}},
  author       = {{Fonseca, Cesar and Spencer-Martins, Isabel and Hahn-Hägerdal, Bärbel}},
  issn         = {{1432-0614}},
  keywords     = {{Hemicellulose; yeast; fermentation conditions; L-Arabinose; pentose; Candida arabinofermentans; Pichia guilliermondii}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{303--310}},
  publisher    = {{Springer}},
  series       = {{Applied Microbiology and Biotechnology}},
  title        = {{L-Arabinose metabolism in Candida arabinofermentans PYCC 5603(T) and Pichia guilliermondii PYCC 3012: influence of sugar and oxygen on product formation}},
  url          = {{http://dx.doi.org/10.1007/s00253-006-0830-7}},
  doi          = {{10.1007/s00253-006-0830-7}},
  volume       = {{75}},
  year         = {{2007}},
}