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Plasticity-enhancing effects of levodopa treatment after stroke

Talhada, Daniela LU ; Marklund, Niklas LU orcid ; Wieloch, Tadeusz LU ; Kuric, Enida LU and Ruscher, Karsten LU (2021) In International Journal of Molecular Sciences 22(19).
Abstract

Dopaminergic treatment in combination with rehabilitative training enhances long-term recovery after stroke. However, the underlying mechanisms on structural plasticity are unknown. Here, we show an increased dopaminergic innervation of the ischemic territory during the first week after stroke induced in Wistar rats subjected to transient occlusion of the middle cerebral ar-tery (tMCAO) for 120 min. This response was also found in rats subjected to permanent focal ische-mia induced by photothrombosis (PT) and mice subjected to PT or tMCAO. Dopaminergic branches were detected in the infarct core of mice and rats in both stroke models. In addition, the Nogo A pathway was significantly downregulated in rats treated with levodopa (LD)... (More)

Dopaminergic treatment in combination with rehabilitative training enhances long-term recovery after stroke. However, the underlying mechanisms on structural plasticity are unknown. Here, we show an increased dopaminergic innervation of the ischemic territory during the first week after stroke induced in Wistar rats subjected to transient occlusion of the middle cerebral ar-tery (tMCAO) for 120 min. This response was also found in rats subjected to permanent focal ische-mia induced by photothrombosis (PT) and mice subjected to PT or tMCAO. Dopaminergic branches were detected in the infarct core of mice and rats in both stroke models. In addition, the Nogo A pathway was significantly downregulated in rats treated with levodopa (LD) compared to vehicle-treated animals subjected to tMCAO. Specifically, the number of Nogo A positive oligodendrocytes as well as the levels of Nogo A and the Nogo A receptor were significantly downregulated in the peri-infarct area of LD-treated animals, while the number of Oligodendrocyte transcription factor 2 positive cells increased in this region after treatment. In addition, we observed lower protein levels of Growth Associated Protein 43 in the peri-infarct area compared to sham-operated animals with-out treatment effect. The results provide the first evidence of the plasticity-promoting actions of dopaminergic treatment following stroke.

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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Dopamine, Neuronal plasticiticy, Nogo A, Nogo receptor, Oligodendro-cyte, Stroke recovery
in
International Journal of Molecular Sciences
volume
22
issue
19
article number
10226
publisher
MDPI AG
external identifiers
  • scopus:85115347699
  • pmid:34638567
ISSN
1661-6596
DOI
10.3390/ijms221910226
language
English
LU publication?
yes
id
cddbf282-f094-416f-a6c4-c653a2401411
date added to LUP
2021-10-08 13:50:28
date last changed
2024-04-20 12:44:28
@article{cddbf282-f094-416f-a6c4-c653a2401411,
  abstract     = {{<p>Dopaminergic treatment in combination with rehabilitative training enhances long-term recovery after stroke. However, the underlying mechanisms on structural plasticity are unknown. Here, we show an increased dopaminergic innervation of the ischemic territory during the first week after stroke induced in Wistar rats subjected to transient occlusion of the middle cerebral ar-tery (tMCAO) for 120 min. This response was also found in rats subjected to permanent focal ische-mia induced by photothrombosis (PT) and mice subjected to PT or tMCAO. Dopaminergic branches were detected in the infarct core of mice and rats in both stroke models. In addition, the Nogo A pathway was significantly downregulated in rats treated with levodopa (LD) compared to vehicle-treated animals subjected to tMCAO. Specifically, the number of Nogo A positive oligodendrocytes as well as the levels of Nogo A and the Nogo A receptor were significantly downregulated in the peri-infarct area of LD-treated animals, while the number of Oligodendrocyte transcription factor 2 positive cells increased in this region after treatment. In addition, we observed lower protein levels of Growth Associated Protein 43 in the peri-infarct area compared to sham-operated animals with-out treatment effect. The results provide the first evidence of the plasticity-promoting actions of dopaminergic treatment following stroke.</p>}},
  author       = {{Talhada, Daniela and Marklund, Niklas and Wieloch, Tadeusz and Kuric, Enida and Ruscher, Karsten}},
  issn         = {{1661-6596}},
  keywords     = {{Dopamine; Neuronal plasticiticy; Nogo A; Nogo receptor; Oligodendro-cyte; Stroke recovery}},
  language     = {{eng}},
  month        = {{10}},
  number       = {{19}},
  publisher    = {{MDPI AG}},
  series       = {{International Journal of Molecular Sciences}},
  title        = {{Plasticity-enhancing effects of levodopa treatment after stroke}},
  url          = {{http://dx.doi.org/10.3390/ijms221910226}},
  doi          = {{10.3390/ijms221910226}},
  volume       = {{22}},
  year         = {{2021}},
}