Integrated acoustic immunoaffinity-capture (IAI) platform for detection of PSA from whole blood samples.
(2013) In Lab on a Chip 13(9). p.1790-1796- Abstract
- On-chip detection of low abundant protein biomarkers is of interest to enable point-of-care diagnostics. Using a simple form of integration, we have realized an integrated microfluidic platform for the detection of prostate specific antigen (PSA), directly in anti-coagulated whole blood. We combine acoustophoresis-based separation of plasma from undiluted whole blood with a miniaturized immunoassay system in a polymer manifold, demonstrating improved assay speed on our Integrated Acoustic Immunoaffinity-capture (IAI) platform. The IAI platform separates plasma from undiluted whole blood by means of acoustophoresis and provides cell free plasma of clinical quality at a rate of 10 uL/min for an online immunoaffinity-capture of PSA on a... (More)
- On-chip detection of low abundant protein biomarkers is of interest to enable point-of-care diagnostics. Using a simple form of integration, we have realized an integrated microfluidic platform for the detection of prostate specific antigen (PSA), directly in anti-coagulated whole blood. We combine acoustophoresis-based separation of plasma from undiluted whole blood with a miniaturized immunoassay system in a polymer manifold, demonstrating improved assay speed on our Integrated Acoustic Immunoaffinity-capture (IAI) platform. The IAI platform separates plasma from undiluted whole blood by means of acoustophoresis and provides cell free plasma of clinical quality at a rate of 10 uL/min for an online immunoaffinity-capture of PSA on a porous silicon antibody microarray. The whole blood input (hematocrit 38-40%) rate was 50 μl min(-1) giving a plasma volume fraction yield of ≈33%. PSA was immunoaffinity-captured directly from spiked female whole blood samples at clinically significant levels of 1.7-100 ng ml(-1) within 15 min and was subsequently detected via fluorescence readout, showing a linear response over the entire range with a coefficient of variation of 13%. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3627852
- author
- Ahmad Tajudin, Asilah LU ; Petersson, Klara LU ; Lenshof, A LU ; Swärd-Nilsson, A-M ; Åberg, Lena LU ; Marko-Varga, György LU ; Malm, Johan LU ; Lilja, Hans LU and Laurell, Thomas LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Lab on a Chip
- volume
- 13
- issue
- 9
- pages
- 1790 - 1796
- publisher
- Royal Society of Chemistry
- external identifiers
-
- wos:000316962700016
- pmid:23515524
- scopus:84875761471
- pmid:23515524
- ISSN
- 1473-0189
- DOI
- 10.1039/c3lc41269e
- language
- English
- LU publication?
- yes
- id
- ce37e203-65be-437d-a2ed-2684e1dd609d (old id 3627852)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/23515524?dopt=Abstract
- date added to LUP
- 2016-04-01 09:52:27
- date last changed
- 2023-11-30 09:52:43
@article{ce37e203-65be-437d-a2ed-2684e1dd609d, abstract = {{On-chip detection of low abundant protein biomarkers is of interest to enable point-of-care diagnostics. Using a simple form of integration, we have realized an integrated microfluidic platform for the detection of prostate specific antigen (PSA), directly in anti-coagulated whole blood. We combine acoustophoresis-based separation of plasma from undiluted whole blood with a miniaturized immunoassay system in a polymer manifold, demonstrating improved assay speed on our Integrated Acoustic Immunoaffinity-capture (IAI) platform. The IAI platform separates plasma from undiluted whole blood by means of acoustophoresis and provides cell free plasma of clinical quality at a rate of 10 uL/min for an online immunoaffinity-capture of PSA on a porous silicon antibody microarray. The whole blood input (hematocrit 38-40%) rate was 50 μl min(-1) giving a plasma volume fraction yield of ≈33%. PSA was immunoaffinity-captured directly from spiked female whole blood samples at clinically significant levels of 1.7-100 ng ml(-1) within 15 min and was subsequently detected via fluorescence readout, showing a linear response over the entire range with a coefficient of variation of 13%.}}, author = {{Ahmad Tajudin, Asilah and Petersson, Klara and Lenshof, A and Swärd-Nilsson, A-M and Åberg, Lena and Marko-Varga, György and Malm, Johan and Lilja, Hans and Laurell, Thomas}}, issn = {{1473-0189}}, language = {{eng}}, number = {{9}}, pages = {{1790--1796}}, publisher = {{Royal Society of Chemistry}}, series = {{Lab on a Chip}}, title = {{Integrated acoustic immunoaffinity-capture (IAI) platform for detection of PSA from whole blood samples.}}, url = {{https://lup.lub.lu.se/search/files/1344817/3737691.pdf}}, doi = {{10.1039/c3lc41269e}}, volume = {{13}}, year = {{2013}}, }