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Integrated acoustic immunoaffinity-capture (IAI) platform for detection of PSA from whole blood samples.

Ahmad Tajudin, Asilah LU ; Petersson, Klara LU ; Lenshof, A LU ; Swärd-Nilsson, A-M ; Åberg, Lena LU ; Marko-Varga, György LU ; Malm, Johan LU ; Lilja, Hans LU orcid and Laurell, Thomas LU (2013) In Lab on a Chip 13(9). p.1790-1796
Abstract
On-chip detection of low abundant protein biomarkers is of interest to enable point-of-care diagnostics. Using a simple form of integration, we have realized an integrated microfluidic platform for the detection of prostate specific antigen (PSA), directly in anti-coagulated whole blood. We combine acoustophoresis-based separation of plasma from undiluted whole blood with a miniaturized immunoassay system in a polymer manifold, demonstrating improved assay speed on our Integrated Acoustic Immunoaffinity-capture (IAI) platform. The IAI platform separates plasma from undiluted whole blood by means of acoustophoresis and provides cell free plasma of clinical quality at a rate of 10 uL/min for an online immunoaffinity-capture of PSA on a... (More)
On-chip detection of low abundant protein biomarkers is of interest to enable point-of-care diagnostics. Using a simple form of integration, we have realized an integrated microfluidic platform for the detection of prostate specific antigen (PSA), directly in anti-coagulated whole blood. We combine acoustophoresis-based separation of plasma from undiluted whole blood with a miniaturized immunoassay system in a polymer manifold, demonstrating improved assay speed on our Integrated Acoustic Immunoaffinity-capture (IAI) platform. The IAI platform separates plasma from undiluted whole blood by means of acoustophoresis and provides cell free plasma of clinical quality at a rate of 10 uL/min for an online immunoaffinity-capture of PSA on a porous silicon antibody microarray. The whole blood input (hematocrit 38-40%) rate was 50 μl min(-1) giving a plasma volume fraction yield of ≈33%. PSA was immunoaffinity-captured directly from spiked female whole blood samples at clinically significant levels of 1.7-100 ng ml(-1) within 15 min and was subsequently detected via fluorescence readout, showing a linear response over the entire range with a coefficient of variation of 13%. (Less)
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author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Lab on a Chip
volume
13
issue
9
pages
1790 - 1796
publisher
Royal Society of Chemistry
external identifiers
  • wos:000316962700016
  • pmid:23515524
  • scopus:84875761471
  • pmid:23515524
ISSN
1473-0189
DOI
10.1039/c3lc41269e
language
English
LU publication?
yes
id
ce37e203-65be-437d-a2ed-2684e1dd609d (old id 3627852)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/23515524?dopt=Abstract
date added to LUP
2016-04-01 09:52:27
date last changed
2023-11-30 09:52:43
@article{ce37e203-65be-437d-a2ed-2684e1dd609d,
  abstract     = {{On-chip detection of low abundant protein biomarkers is of interest to enable point-of-care diagnostics. Using a simple form of integration, we have realized an integrated microfluidic platform for the detection of prostate specific antigen (PSA), directly in anti-coagulated whole blood. We combine acoustophoresis-based separation of plasma from undiluted whole blood with a miniaturized immunoassay system in a polymer manifold, demonstrating improved assay speed on our Integrated Acoustic Immunoaffinity-capture (IAI) platform. The IAI platform separates plasma from undiluted whole blood by means of acoustophoresis and provides cell free plasma of clinical quality at a rate of 10 uL/min for an online immunoaffinity-capture of PSA on a porous silicon antibody microarray. The whole blood input (hematocrit 38-40%) rate was 50 μl min(-1) giving a plasma volume fraction yield of ≈33%. PSA was immunoaffinity-captured directly from spiked female whole blood samples at clinically significant levels of 1.7-100 ng ml(-1) within 15 min and was subsequently detected via fluorescence readout, showing a linear response over the entire range with a coefficient of variation of 13%.}},
  author       = {{Ahmad Tajudin, Asilah and Petersson, Klara and Lenshof, A and Swärd-Nilsson, A-M and Åberg, Lena and Marko-Varga, György and Malm, Johan and Lilja, Hans and Laurell, Thomas}},
  issn         = {{1473-0189}},
  language     = {{eng}},
  number       = {{9}},
  pages        = {{1790--1796}},
  publisher    = {{Royal Society of Chemistry}},
  series       = {{Lab on a Chip}},
  title        = {{Integrated acoustic immunoaffinity-capture (IAI) platform for detection of PSA from whole blood samples.}},
  url          = {{https://lup.lub.lu.se/search/files/1344817/3737691.pdf}},
  doi          = {{10.1039/c3lc41269e}},
  volume       = {{13}},
  year         = {{2013}},
}