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UVB irradiation induces rapid changes in galanin, substance P and c-fos immunoreactivity in rat dorsal root ganglia and spinal cord

Etemadi, Leila LU ; Pettersson, Lina M E LU and Danielsen, Nils LU (2017) In Peptides 87. p.71-83
Abstract

Recent studies have shown that UVB irradiation induces primary and secondary hyperalgesia in rats and humans peaking about 24 h after UVB exposure. In the present study we investigated the changes in galanin, substance P and c-fos immunoreactivity in rat DRG and spinal cord at the L5 level 2–96 h after UVB irradiation. UVB irradiation of the heel area in rats almost increased the skin blood flow two-fold 24 h after irradiation as measured by laser Doppler technique. UVB irradiation induced a significant reduction of the proportion of galanin positive DRG neurons for all time points, except at 12 h. In the spinal cord, UVB irradiation induced increased immunoreactivity for galanin in the dorsal horn, the area around the central canal and... (More)

Recent studies have shown that UVB irradiation induces primary and secondary hyperalgesia in rats and humans peaking about 24 h after UVB exposure. In the present study we investigated the changes in galanin, substance P and c-fos immunoreactivity in rat DRG and spinal cord at the L5 level 2–96 h after UVB irradiation. UVB irradiation of the heel area in rats almost increased the skin blood flow two-fold 24 h after irradiation as measured by laser Doppler technique. UVB irradiation induced a significant reduction of the proportion of galanin positive DRG neurons for all time points, except at 12 h. In the spinal cord, UVB irradiation induced increased immunoreactivity for galanin in the dorsal horn, the area around the central canal and interestingly also in the lateral spinal nucleus 12–96 h after exposure. For substance P the proportion of substance P positive neurons was unchanged but UVB irradiation induced increased substance P immunoreactivity in the dorsal part of the spinal cord 48 h after irradiation. UVB irradiation also induced c-fos immunoreactivity in the dorsal horn and the area around the central canal 24 and 48 h after exposure. This translational model of UVB irradiation will induce rapid changes of neuropeptides implicated in nociceptive signaling in areas known to be of importance for nociception in a time frame, about 24 h after exposure, where also neurophysiological alteration have been described in humans and rats.

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organization
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type
Contribution to journal
publication status
published
subject
keywords
Dorsal horn, Immunoreactivity, Inflammation, Lateral spinal nucleus, Neuropeptides, Pain translational model
in
Peptides
volume
87
pages
13 pages
publisher
Elsevier
external identifiers
  • scopus:85002683488
  • wos:000392684000009
ISSN
0196-9781
DOI
10.1016/j.peptides.2016.12.001
language
English
LU publication?
yes
id
ce396b55-b8b7-4d7b-a46a-c8afef872479
date added to LUP
2017-03-17 09:35:03
date last changed
2018-01-07 11:56:10
@article{ce396b55-b8b7-4d7b-a46a-c8afef872479,
  abstract     = {<p>Recent studies have shown that UVB irradiation induces primary and secondary hyperalgesia in rats and humans peaking about 24 h after UVB exposure. In the present study we investigated the changes in galanin, substance P and c-fos immunoreactivity in rat DRG and spinal cord at the L5 level 2–96 h after UVB irradiation. UVB irradiation of the heel area in rats almost increased the skin blood flow two-fold 24 h after irradiation as measured by laser Doppler technique. UVB irradiation induced a significant reduction of the proportion of galanin positive DRG neurons for all time points, except at 12 h. In the spinal cord, UVB irradiation induced increased immunoreactivity for galanin in the dorsal horn, the area around the central canal and interestingly also in the lateral spinal nucleus 12–96 h after exposure. For substance P the proportion of substance P positive neurons was unchanged but UVB irradiation induced increased substance P immunoreactivity in the dorsal part of the spinal cord 48 h after irradiation. UVB irradiation also induced c-fos immunoreactivity in the dorsal horn and the area around the central canal 24 and 48 h after exposure. This translational model of UVB irradiation will induce rapid changes of neuropeptides implicated in nociceptive signaling in areas known to be of importance for nociception in a time frame, about 24 h after exposure, where also neurophysiological alteration have been described in humans and rats.</p>},
  author       = {Etemadi, Leila and Pettersson, Lina M E and Danielsen, Nils},
  issn         = {0196-9781},
  keyword      = {Dorsal horn,Immunoreactivity,Inflammation,Lateral spinal nucleus,Neuropeptides,Pain translational model},
  language     = {eng},
  month        = {01},
  pages        = {71--83},
  publisher    = {Elsevier},
  series       = {Peptides},
  title        = {UVB irradiation induces rapid changes in galanin, substance P and c-fos immunoreactivity in rat dorsal root ganglia and spinal cord},
  url          = {http://dx.doi.org/10.1016/j.peptides.2016.12.001},
  volume       = {87},
  year         = {2017},
}