Identification, Characterization, and Antibacterial Evaluation of Five Endophytic Fungi from Psychotria poeppigiana Müll. Arg., an Amazon Plant
Mendieta-Brito, Sonia ; Sayed, Mahmoud LU
; Son, Eunjung
; Kim, Dong-Seon
; Dávila, Marcelo
and Pyo, Sang-Hyun
LU
(2024)
In Microorganisms
12(8).
- Abstract
- Endophytic fungi, residing within plants without causing disease, are known for their ability to produce bioactive metabolites with diverse properties such as antibacterial, antioxidant, and antifungal activities, while also influencing plant defense mechanisms. In this study, five novel endophytic fungi species were isolated from the leaves of Psychotria poeppigiana Müll. Arg., a plant from the Rubiaceae family, collected in the tropical Amazon region of Bolivia. The endophytic fungi were identified as a Neopestalotiopsis sp., three Penicillium sp., and an Aspergillus sp. through 18S ribosomal RNA sequencing and NCBI-BLAST analysis. Chemical profiling revealed that their extracts obtained by ethyl acetate contained terpenes, flavonoids,... (More)
- Endophytic fungi, residing within plants without causing disease, are known for their ability to produce bioactive metabolites with diverse properties such as antibacterial, antioxidant, and antifungal activities, while also influencing plant defense mechanisms. In this study, five novel endophytic fungi species were isolated from the leaves of Psychotria poeppigiana Müll. Arg., a plant from the Rubiaceae family, collected in the tropical Amazon region of Bolivia. The endophytic fungi were identified as a Neopestalotiopsis sp., three Penicillium sp., and an Aspergillus sp. through 18S ribosomal RNA sequencing and NCBI-BLAST analysis. Chemical profiling revealed that their extracts obtained by ethyl acetate contained terpenes, flavonoids, and phenolic compounds. In a bioautography study, the terpenes showed high antimicrobial activity against Escherichia coli. Notably, extracts from the three Penicillium species exhibited potent antibacterial activity, with minimum inhibitory concentration (MIC) values ranging from 62.5 to 2000 µg/mL against all three pathogens: Escherichia coli, Staphylococcus aureus, and Enterococcus faecalis (both Gram-positive and Gram-negative bacteria). These findings highlight the potential of these endophytic fungi, especially Penicillium species as valuable sources of secondary metabolites with significant antibacterial activities, suggesting promising applications in medicine, pharmaceuticals, agriculture, and environmental technologies. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/ce9281d2-4724-497a-a248-06c9f7d5792b
- author
- Mendieta-Brito, Sonia
; Sayed, Mahmoud
LU
; Son, Eunjung
; Kim, Dong-Seon
; Dávila, Marcelo
and Pyo, Sang-Hyun
LU
- organization
- publishing date
- 2024-08-05
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Microorganisms
- volume
- 12
- issue
- 8
- article number
- 1590
- pages
- 24 pages
- publisher
- MDPI AG
- external identifiers
-
- pmid:39203432
- scopus:85202675926
- ISSN
- 2076-2607
- DOI
- 10.3390/microorganisms12081590
- language
- English
- LU publication?
- yes
- id
- ce9281d2-4724-497a-a248-06c9f7d5792b
- date added to LUP
- 2024-09-29 14:22:36
- date last changed
- 2024-10-03 14:09:49
@article{ce9281d2-4724-497a-a248-06c9f7d5792b,
abstract = {{Endophytic fungi, residing within plants without causing disease, are known for their ability to produce bioactive metabolites with diverse properties such as antibacterial, antioxidant, and antifungal activities, while also influencing plant defense mechanisms. In this study, five novel endophytic fungi species were isolated from the leaves of Psychotria poeppigiana Müll. Arg., a plant from the Rubiaceae family, collected in the tropical Amazon region of Bolivia. The endophytic fungi were identified as a Neopestalotiopsis sp., three Penicillium sp., and an Aspergillus sp. through 18S ribosomal RNA sequencing and NCBI-BLAST analysis. Chemical profiling revealed that their extracts obtained by ethyl acetate contained terpenes, flavonoids, and phenolic compounds. In a bioautography study, the terpenes showed high antimicrobial activity against Escherichia coli. Notably, extracts from the three Penicillium species exhibited potent antibacterial activity, with minimum inhibitory concentration (MIC) values ranging from 62.5 to 2000 µg/mL against all three pathogens: Escherichia coli, Staphylococcus aureus, and Enterococcus faecalis (both Gram-positive and Gram-negative bacteria). These findings highlight the potential of these endophytic fungi, especially Penicillium species as valuable sources of secondary metabolites with significant antibacterial activities, suggesting promising applications in medicine, pharmaceuticals, agriculture, and environmental technologies.}},
author = {{Mendieta-Brito, Sonia and Sayed, Mahmoud and Son, Eunjung and Kim, Dong-Seon and Dávila, Marcelo and Pyo, Sang-Hyun}},
issn = {{2076-2607}},
language = {{eng}},
month = {{08}},
number = {{8}},
publisher = {{MDPI AG}},
series = {{Microorganisms}},
title = {{Identification, Characterization, and Antibacterial Evaluation of Five Endophytic Fungi from <i>Psychotria poeppigiana</i> Müll. Arg., an Amazon Plant}},
url = {{http://dx.doi.org/10.3390/microorganisms12081590}},
doi = {{10.3390/microorganisms12081590}},
volume = {{12}},
year = {{2024}},
}