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Quantitative high-performance liquid chromatography-tandem mass spectrometry method for the analysis of free desmosines in plasma and urine

Miliotis, Tasso; Lindberg, Claes; Semb, Kristina Forsman; van Geest, Marleen and Kjellström, Sven LU (2013) In Journal of Chromatography A 1308. p.73-78
Abstract
A rapid method for the determination of the sum of free desmosine and isodesmosine in human plasma and urine is described. Efficient sample clean-up prior to LC-MS/MS analysis is mandatory for detection of free desmosines in plasma samples. The combination of ultra-filtration and a two-step solid phase extraction minimizes the sample complexity and ion suppression effects. The flow through from the ultra filtration is passed through a C18 resin and then the target analytes are trapped and enriched on a mixed mode solid phase extraction material. The combination of these three orthogonal sample preparation steps allows detection of endogenous free desmosines in plasma from healthy individuals. An analytical column packed with porous... (More)
A rapid method for the determination of the sum of free desmosine and isodesmosine in human plasma and urine is described. Efficient sample clean-up prior to LC-MS/MS analysis is mandatory for detection of free desmosines in plasma samples. The combination of ultra-filtration and a two-step solid phase extraction minimizes the sample complexity and ion suppression effects. The flow through from the ultra filtration is passed through a C18 resin and then the target analytes are trapped and enriched on a mixed mode solid phase extraction material. The combination of these three orthogonal sample preparation steps allows detection of endogenous free desmosines in plasma from healthy individuals. An analytical column packed with porous graphitic carbon material enables the retention of the polar desmosine analytes, which are measured by electrospray ionization tandem mass spectrometry. Deuterium labeled isodesmosine is added as internal standard and a linear calibration curve was constructed in the range of 0.1-2.0 nmol/L for plasma samples and 5-200 nmol/L for urine samples. These results demonstrate that the described LC-MS/MS method provides sensitive, repeatable and accurate quantification of free desmosines in plasma and urine samples. (C) 2013 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Free desmosines, Quantification, LC-MS/MS
in
Journal of Chromatography A
volume
1308
pages
73 - 78
publisher
Elsevier
external identifiers
  • wos:000324353100009
  • scopus:84883055163
ISSN
0021-9673
DOI
10.1016/j.chroma.2013.06.063
language
English
LU publication?
yes
id
cfb38dc9-fbf7-4c78-935d-fdf5659f6521 (old id 4098919)
date added to LUP
2013-10-29 09:24:36
date last changed
2019-02-20 06:30:34
@article{cfb38dc9-fbf7-4c78-935d-fdf5659f6521,
  abstract     = {A rapid method for the determination of the sum of free desmosine and isodesmosine in human plasma and urine is described. Efficient sample clean-up prior to LC-MS/MS analysis is mandatory for detection of free desmosines in plasma samples. The combination of ultra-filtration and a two-step solid phase extraction minimizes the sample complexity and ion suppression effects. The flow through from the ultra filtration is passed through a C18 resin and then the target analytes are trapped and enriched on a mixed mode solid phase extraction material. The combination of these three orthogonal sample preparation steps allows detection of endogenous free desmosines in plasma from healthy individuals. An analytical column packed with porous graphitic carbon material enables the retention of the polar desmosine analytes, which are measured by electrospray ionization tandem mass spectrometry. Deuterium labeled isodesmosine is added as internal standard and a linear calibration curve was constructed in the range of 0.1-2.0 nmol/L for plasma samples and 5-200 nmol/L for urine samples. These results demonstrate that the described LC-MS/MS method provides sensitive, repeatable and accurate quantification of free desmosines in plasma and urine samples. (C) 2013 Elsevier B.V. All rights reserved.},
  author       = {Miliotis, Tasso and Lindberg, Claes and Semb, Kristina Forsman and van Geest, Marleen and Kjellström, Sven},
  issn         = {0021-9673},
  keyword      = {Free desmosines,Quantification,LC-MS/MS},
  language     = {eng},
  pages        = {73--78},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography A},
  title        = {Quantitative high-performance liquid chromatography-tandem mass spectrometry method for the analysis of free desmosines in plasma and urine},
  url          = {http://dx.doi.org/10.1016/j.chroma.2013.06.063},
  volume       = {1308},
  year         = {2013},
}