Quantitative high-performance liquid chromatography-tandem mass spectrometry method for the analysis of free desmosines in plasma and urine
(2013) In Journal of Chromatography A 1308. p.73-78- Abstract
- A rapid method for the determination of the sum of free desmosine and isodesmosine in human plasma and urine is described. Efficient sample clean-up prior to LC-MS/MS analysis is mandatory for detection of free desmosines in plasma samples. The combination of ultra-filtration and a two-step solid phase extraction minimizes the sample complexity and ion suppression effects. The flow through from the ultra filtration is passed through a C18 resin and then the target analytes are trapped and enriched on a mixed mode solid phase extraction material. The combination of these three orthogonal sample preparation steps allows detection of endogenous free desmosines in plasma from healthy individuals. An analytical column packed with porous... (More)
- A rapid method for the determination of the sum of free desmosine and isodesmosine in human plasma and urine is described. Efficient sample clean-up prior to LC-MS/MS analysis is mandatory for detection of free desmosines in plasma samples. The combination of ultra-filtration and a two-step solid phase extraction minimizes the sample complexity and ion suppression effects. The flow through from the ultra filtration is passed through a C18 resin and then the target analytes are trapped and enriched on a mixed mode solid phase extraction material. The combination of these three orthogonal sample preparation steps allows detection of endogenous free desmosines in plasma from healthy individuals. An analytical column packed with porous graphitic carbon material enables the retention of the polar desmosine analytes, which are measured by electrospray ionization tandem mass spectrometry. Deuterium labeled isodesmosine is added as internal standard and a linear calibration curve was constructed in the range of 0.1-2.0 nmol/L for plasma samples and 5-200 nmol/L for urine samples. These results demonstrate that the described LC-MS/MS method provides sensitive, repeatable and accurate quantification of free desmosines in plasma and urine samples. (C) 2013 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4098919
- author
- Miliotis, Tasso ; Lindberg, Claes ; Semb, Kristina Forsman ; van Geest, Marleen and Kjellström, Sven LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Free desmosines, Quantification, LC-MS/MS
- in
- Journal of Chromatography A
- volume
- 1308
- pages
- 73 - 78
- publisher
- Elsevier
- external identifiers
-
- wos:000324353100009
- scopus:84883055163
- pmid:23953714
- ISSN
- 0021-9673
- DOI
- 10.1016/j.chroma.2013.06.063
- language
- English
- LU publication?
- yes
- id
- cfb38dc9-fbf7-4c78-935d-fdf5659f6521 (old id 4098919)
- date added to LUP
- 2016-04-01 14:19:13
- date last changed
- 2022-04-14 17:11:18
@article{cfb38dc9-fbf7-4c78-935d-fdf5659f6521,
abstract = {{A rapid method for the determination of the sum of free desmosine and isodesmosine in human plasma and urine is described. Efficient sample clean-up prior to LC-MS/MS analysis is mandatory for detection of free desmosines in plasma samples. The combination of ultra-filtration and a two-step solid phase extraction minimizes the sample complexity and ion suppression effects. The flow through from the ultra filtration is passed through a C18 resin and then the target analytes are trapped and enriched on a mixed mode solid phase extraction material. The combination of these three orthogonal sample preparation steps allows detection of endogenous free desmosines in plasma from healthy individuals. An analytical column packed with porous graphitic carbon material enables the retention of the polar desmosine analytes, which are measured by electrospray ionization tandem mass spectrometry. Deuterium labeled isodesmosine is added as internal standard and a linear calibration curve was constructed in the range of 0.1-2.0 nmol/L for plasma samples and 5-200 nmol/L for urine samples. These results demonstrate that the described LC-MS/MS method provides sensitive, repeatable and accurate quantification of free desmosines in plasma and urine samples. (C) 2013 Elsevier B.V. All rights reserved.}},
author = {{Miliotis, Tasso and Lindberg, Claes and Semb, Kristina Forsman and van Geest, Marleen and Kjellström, Sven}},
issn = {{0021-9673}},
keywords = {{Free desmosines; Quantification; LC-MS/MS}},
language = {{eng}},
pages = {{73--78}},
publisher = {{Elsevier}},
series = {{Journal of Chromatography A}},
title = {{Quantitative high-performance liquid chromatography-tandem mass spectrometry method for the analysis of free desmosines in plasma and urine}},
url = {{http://dx.doi.org/10.1016/j.chroma.2013.06.063}},
doi = {{10.1016/j.chroma.2013.06.063}},
volume = {{1308}},
year = {{2013}},
}