Genes for cytochrome c and cytochrome c oxidase synthesis in Bacillus subtilis
(2001)- Abstract
- Cytochromes are heme-containing proteins that function in electron transfer reactions, e.g. respiration and photosynthesis. There are several different types of cytochromes. C-type cytochromes differ from other types of cytochromes in that the heme group is covalently attached to the polypeptide via thioether bonds to two cysteinyl residues in a consensus motif, Cys-Xaa-Yaa-Cys-His. Cytochrome c oxidase is an energy transducing membrane-bound enzyme with two heme groups and two copper centres. This enzyme reduces molecular oxygen to water and is essential for animals, plants and many micro-organisms. This thesis addresses questions about how cytochrome c and cytochrome c oxidases are synthesised in living cells. From these studies one also... (More)
- Cytochromes are heme-containing proteins that function in electron transfer reactions, e.g. respiration and photosynthesis. There are several different types of cytochromes. C-type cytochromes differ from other types of cytochromes in that the heme group is covalently attached to the polypeptide via thioether bonds to two cysteinyl residues in a consensus motif, Cys-Xaa-Yaa-Cys-His. Cytochrome c oxidase is an energy transducing membrane-bound enzyme with two heme groups and two copper centres. This enzyme reduces molecular oxygen to water and is essential for animals, plants and many micro-organisms. This thesis addresses questions about how cytochrome c and cytochrome c oxidases are synthesised in living cells. From these studies one also obtains general knowledge about the biosynthesis of other types of metal-containing proteins. The Gram-positive soil bacterium Bacillus subtilis was previously known to contain three c-type cytochromes: QcrC, the cytochrome c of the bc complex; CtaC, the subunit II of cytochrome c oxidase; and CccA, the cytochrome c550. In the thesis it is demonstrated that the cccB gene encodes a fourth cytochrome c, cytochrome c551. Furthermore it is shown that CtaC is a lipoprotein and that the processing of the polypeptide is important for the function of cytochrome c oxidase. By screening different libraries of mutants for deficiency in functional cytochrome c oxidase and lack of all cytochrome c the proteins encoded by the resB and resC genes were found to be important for cytochrome c synthesis in B. subtilis. Two genes, ctaG and ypmQ, were found to encode proteins important for synthesis of functional cytochrome c oxidase. (Less)
- Abstract (Swedish)
- Popular Abstract in Swedish
Cytokromer är hem-innehållande proteiner som transporterar elektroner. Hem är den järninnehållande molekyl som binder syre i hemoglobin i de röda blodkropparna i blodet hos t ex människor. Cytokromer har viktiga funktioner i olika typer av organismer: bakterier, växter och djur. Genom att studera vilka gener som behövs för syntesen av cytokromer i bakterien Bacillus subtilis kan vi lära oss om hur syntesen av cytokromer och andra metallinnehållande proteiner fungerar i andra organismer samt lära oss mer om hur bakterien Bacillus subtilis fungerar.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/41866
- author
- Bengtsson, Jenny LU
- supervisor
- opponent
-
- Prof Gennis, Robert B., Dept. of Biochemistry, School of Chemical Sciences, University of Illinois, USA
- organization
- publishing date
- 2001
- type
- Thesis
- publication status
- published
- subject
- keywords
- Microbiology, cytochrome, cytochrome c oxidase, Bacillus subtilis, cytochrome c synthesis, bacteriology, virology, mycology, Mikrobiologi, bakteriologi, virologi, mykologi
- pages
- 136 pages
- publisher
- Department of Microbiology, Lund University
- defense location
- Rune Grubb hall, BMC, Sölveg 21, Lund
- defense date
- 2001-10-28 10:00:00
- external identifiers
-
- other:ISRN: LUNBDS/NBNB-1040/1-75 (2001)
- ISBN
- 91-628-4902-6
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Biology building (Closed 2011) (011008000)
- id
- d02421d7-bda6-4f40-bbe4-bbee9332f709 (old id 41866)
- date added to LUP
- 2016-04-04 10:17:02
- date last changed
- 2018-11-21 20:57:53
@phdthesis{d02421d7-bda6-4f40-bbe4-bbee9332f709,
abstract = {{Cytochromes are heme-containing proteins that function in electron transfer reactions, e.g. respiration and photosynthesis. There are several different types of cytochromes. C-type cytochromes differ from other types of cytochromes in that the heme group is covalently attached to the polypeptide via thioether bonds to two cysteinyl residues in a consensus motif, Cys-Xaa-Yaa-Cys-His. Cytochrome c oxidase is an energy transducing membrane-bound enzyme with two heme groups and two copper centres. This enzyme reduces molecular oxygen to water and is essential for animals, plants and many micro-organisms. This thesis addresses questions about how cytochrome c and cytochrome c oxidases are synthesised in living cells. From these studies one also obtains general knowledge about the biosynthesis of other types of metal-containing proteins. The Gram-positive soil bacterium Bacillus subtilis was previously known to contain three c-type cytochromes: QcrC, the cytochrome c of the bc complex; CtaC, the subunit II of cytochrome c oxidase; and CccA, the cytochrome c550. In the thesis it is demonstrated that the cccB gene encodes a fourth cytochrome c, cytochrome c551. Furthermore it is shown that CtaC is a lipoprotein and that the processing of the polypeptide is important for the function of cytochrome c oxidase. By screening different libraries of mutants for deficiency in functional cytochrome c oxidase and lack of all cytochrome c the proteins encoded by the resB and resC genes were found to be important for cytochrome c synthesis in B. subtilis. Two genes, ctaG and ypmQ, were found to encode proteins important for synthesis of functional cytochrome c oxidase.}},
author = {{Bengtsson, Jenny}},
isbn = {{91-628-4902-6}},
keywords = {{Microbiology; cytochrome; cytochrome c oxidase; Bacillus subtilis; cytochrome c synthesis; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi}},
language = {{eng}},
publisher = {{Department of Microbiology, Lund University}},
school = {{Lund University}},
title = {{Genes for cytochrome c and cytochrome c oxidase synthesis in Bacillus subtilis}},
year = {{2001}},
}