A distinct Toll-like receptor repertoire in human tonsillar B cells, directly activated by PamCSK, R-837 and CpG-2006 stimulation.
(2006) In Immunology 118(Jun 16). p.539-548- Abstract
- Toll-like receptors (TLRs) recognize specific pathogen-associated molecular patterns (PAMPs), which subsequently trigger innate immunity. Recent data also suggest a role for TLRs in the direct activation of adaptive immune cells. In the present study, the expression and function of TLR1-TLR10 were characterized in purified human tonsillar B cells, focusing on differences among CD19(+) CD38(-) CD27(-) (naive B cells), CD19(+) IgD(-) CD27- [germinal centre (GC) B cells] and CD19(+) CD38(-)CD27(+) (memory B cells) cells. The study was also designed to compare the TLR expression in B cells obtained from infected and hyperplastic tonsils that served as controls. The results demonstrated a distinct repertoire of TLRs, in which TLR1, TLR2, TLR7,... (More)
- Toll-like receptors (TLRs) recognize specific pathogen-associated molecular patterns (PAMPs), which subsequently trigger innate immunity. Recent data also suggest a role for TLRs in the direct activation of adaptive immune cells. In the present study, the expression and function of TLR1-TLR10 were characterized in purified human tonsillar B cells, focusing on differences among CD19(+) CD38(-) CD27(-) (naive B cells), CD19(+) IgD(-) CD27- [germinal centre (GC) B cells] and CD19(+) CD38(-)CD27(+) (memory B cells) cells. The study was also designed to compare the TLR expression in B cells obtained from infected and hyperplastic tonsils that served as controls. The results demonstrated a distinct repertoire of TLRs, in which TLR1, TLR2, TLR7, TLR9 and TLR10 predominated. No differences were found among naive, GC and memory B cells. Tonsillar infection did not substantially alter the TLR expression profile in ex vivo-isolated B-cell subsets. Purified CD19+ B cells stimulated with Pam(3)CSK(4), R-837 and CpG oligodeoxynucleotide (ODN) 2006, via TLR1/TLR2, TLR7 and TLR9, respectively, showed an induction of interleukin-6 secretion and an up-regulated expression of human leucocyte antigen (HLA)-DR. Collectively, the present study demonstrates that B cells exhibit constitutively high levels of specific TLRs, which are not developmentally regulated during the B-cell differentiation process. Ongoing microbial infections, such as chronic tonsillitis, do not appear to affect the TLR profile in B cells. Furthermore, the distinct expression of TLRs allows B cells to.respond directly to the cognate PAMPs. This further emphasizes the role of TLRs in directly activating adaptive immune cells. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/158282
- author
- Månsson, Anne LU ; Adner, Mikael LU ; Höckerfelt, Ulf LU and Cardell, Lars-Olaf LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- tonsillar B cells, infection, PAMPs, Toll-like receptors
- in
- Immunology
- volume
- 118
- issue
- Jun 16
- pages
- 539 - 548
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000239961600011
- pmid:16780564
- scopus:33746053199
- ISSN
- 0019-2805
- DOI
- 10.1111/j.1365-2567.2006.02392.x
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Clinical and Experimental Allergy Research (013243510), Reconstructive Surgery (013240300)
- id
- d0476981-e2f1-4e7a-a362-472fd3ca13c2 (old id 158282)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16780564&dopt=Abstract
- date added to LUP
- 2016-04-01 12:14:42
- date last changed
- 2022-04-21 04:42:23
@article{d0476981-e2f1-4e7a-a362-472fd3ca13c2,
abstract = {{Toll-like receptors (TLRs) recognize specific pathogen-associated molecular patterns (PAMPs), which subsequently trigger innate immunity. Recent data also suggest a role for TLRs in the direct activation of adaptive immune cells. In the present study, the expression and function of TLR1-TLR10 were characterized in purified human tonsillar B cells, focusing on differences among CD19(+) CD38(-) CD27(-) (naive B cells), CD19(+) IgD(-) CD27- [germinal centre (GC) B cells] and CD19(+) CD38(-)CD27(+) (memory B cells) cells. The study was also designed to compare the TLR expression in B cells obtained from infected and hyperplastic tonsils that served as controls. The results demonstrated a distinct repertoire of TLRs, in which TLR1, TLR2, TLR7, TLR9 and TLR10 predominated. No differences were found among naive, GC and memory B cells. Tonsillar infection did not substantially alter the TLR expression profile in ex vivo-isolated B-cell subsets. Purified CD19+ B cells stimulated with Pam(3)CSK(4), R-837 and CpG oligodeoxynucleotide (ODN) 2006, via TLR1/TLR2, TLR7 and TLR9, respectively, showed an induction of interleukin-6 secretion and an up-regulated expression of human leucocyte antigen (HLA)-DR. Collectively, the present study demonstrates that B cells exhibit constitutively high levels of specific TLRs, which are not developmentally regulated during the B-cell differentiation process. Ongoing microbial infections, such as chronic tonsillitis, do not appear to affect the TLR profile in B cells. Furthermore, the distinct expression of TLRs allows B cells to.respond directly to the cognate PAMPs. This further emphasizes the role of TLRs in directly activating adaptive immune cells.}},
author = {{Månsson, Anne and Adner, Mikael and Höckerfelt, Ulf and Cardell, Lars-Olaf}},
issn = {{0019-2805}},
keywords = {{tonsillar B cells; infection; PAMPs; Toll-like receptors}},
language = {{eng}},
number = {{Jun 16}},
pages = {{539--548}},
publisher = {{Wiley-Blackwell}},
series = {{Immunology}},
title = {{A distinct Toll-like receptor repertoire in human tonsillar B cells, directly activated by PamCSK, R-837 and CpG-2006 stimulation.}},
url = {{https://lup.lub.lu.se/search/files/2843086/625500.pdf}},
doi = {{10.1111/j.1365-2567.2006.02392.x}},
volume = {{118}},
year = {{2006}},
}