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Acoustophoresis separation of bacteria from blood cells for rapid sepsis diagnostics

Ohlsson, P. D. LU ; Petersson, K. LU ; Augustsson, P. LU and Laurell, T. LU (2013) 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 2. p.1320-1322
Abstract

We present a significantly improved acoustophoresis method to separate bacteria from red and white blood cells. As much as 98% of the bacteria were recovered, while only 0.06 % of the red and white blood cells remained. This 1600-fold relative enrichment was achieved by adjusting the center medium density, tightly controlling the chip temperature and stabilizing the fluid flows. This is a crucial sample preparation step facilitating downstream bacteria identification for sepsis diagnostics.

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author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Acoustophoresis, Bacteria, Blood, Sepsis
in
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
volume
2
pages
3 pages
publisher
Chemical and Biological Microsystems Society
conference name
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
external identifiers
  • scopus:84907358599
ISBN
9781632666246
language
English
LU publication?
yes
id
d060ebb4-ab6d-4c47-965a-9188673c4049
date added to LUP
2017-12-06 15:17:22
date last changed
2018-01-07 12:27:23
@inproceedings{d060ebb4-ab6d-4c47-965a-9188673c4049,
  abstract     = {<p>We present a significantly improved acoustophoresis method to separate bacteria from red and white blood cells. As much as 98% of the bacteria were recovered, while only 0.06 % of the red and white blood cells remained. This 1600-fold relative enrichment was achieved by adjusting the center medium density, tightly controlling the chip temperature and stabilizing the fluid flows. This is a crucial sample preparation step facilitating downstream bacteria identification for sepsis diagnostics.</p>},
  author       = {Ohlsson, P. D. and Petersson, K. and Augustsson, P. and Laurell, T.},
  booktitle    = {17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013},
  isbn         = {9781632666246},
  keyword      = {Acoustophoresis,Bacteria,Blood,Sepsis},
  language     = {eng},
  pages        = {1320--1322},
  publisher    = {Chemical and Biological Microsystems Society},
  title        = {Acoustophoresis separation of bacteria from blood cells for rapid sepsis diagnostics},
  volume       = {2},
  year         = {2013},
}