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Acoustophoresis separation of bacteria from blood cells for rapid sepsis diagnostics

Ohlsson, P. D. LU ; Petersson, K. LU ; Augustsson, P. LU and Laurell, T. LU (2013) 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 2. p.1320-1322
Abstract

We present a significantly improved acoustophoresis method to separate bacteria from red and white blood cells. As much as 98% of the bacteria were recovered, while only 0.06 % of the red and white blood cells remained. This 1600-fold relative enrichment was achieved by adjusting the center medium density, tightly controlling the chip temperature and stabilizing the fluid flows. This is a crucial sample preparation step facilitating downstream bacteria identification for sepsis diagnostics.

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author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Acoustophoresis, Bacteria, Blood, Sepsis
host publication
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
volume
2
pages
3 pages
publisher
Chemical and Biological Microsystems Society
conference name
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
conference location
Freiburg, Germany
conference dates
2013-10-27 - 2013-10-31
external identifiers
  • scopus:84907358599
ISBN
9781632666246
language
English
LU publication?
yes
id
d060ebb4-ab6d-4c47-965a-9188673c4049
date added to LUP
2017-12-06 15:17:22
date last changed
2019-01-06 13:35:53
@inproceedings{d060ebb4-ab6d-4c47-965a-9188673c4049,
  abstract     = {<p>We present a significantly improved acoustophoresis method to separate bacteria from red and white blood cells. As much as 98% of the bacteria were recovered, while only 0.06 % of the red and white blood cells remained. This 1600-fold relative enrichment was achieved by adjusting the center medium density, tightly controlling the chip temperature and stabilizing the fluid flows. This is a crucial sample preparation step facilitating downstream bacteria identification for sepsis diagnostics.</p>},
  author       = {Ohlsson, P. D. and Petersson, K. and Augustsson, P. and Laurell, T.},
  isbn         = {9781632666246},
  keyword      = {Acoustophoresis,Bacteria,Blood,Sepsis},
  language     = {eng},
  location     = {Freiburg, Germany},
  pages        = {1320--1322},
  publisher    = {Chemical and Biological Microsystems Society},
  title        = {Acoustophoresis separation of bacteria from blood cells for rapid sepsis diagnostics},
  volume       = {2},
  year         = {2013},
}