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A graphene-based glycan biosensor for electrochemical label-free detection of a tumor-associated antibody

Kveton, Filip ; Blsakova, Anna ; Lorencova, Lenka ; Jerigova, Monika ; Velic, Dusan ; Blixt, Ola ; Jansson, Bo LU ; Kasak, Peter and Tkac, Jan LU (2019) In Sensors (Switzerland) 19(24).
Abstract

The study describes development of a glycan biosensor for detection of a tumor-associated antibody. The glycan biosensor is built on an electrochemically activated/oxidized graphene screen-printed electrode (GSPE). Oxygen functionalities were subsequently applied for covalent immobilization of human serum albumin (HSA) as a natural nanoscaffold for covalent immobilization of Thomsen-nouvelle (Tn) antigen (GalNAc-O-Ser/Thr) to be fully available for affinity interaction with its analyte—a tumor-associated antibody. The step by step building process of glycan biosensor development was comprehensively characterized using a battery of techniques (scanning electron microscopy, atomic force microscopy, contact angle measurements, secondary... (More)

The study describes development of a glycan biosensor for detection of a tumor-associated antibody. The glycan biosensor is built on an electrochemically activated/oxidized graphene screen-printed electrode (GSPE). Oxygen functionalities were subsequently applied for covalent immobilization of human serum albumin (HSA) as a natural nanoscaffold for covalent immobilization of Thomsen-nouvelle (Tn) antigen (GalNAc-O-Ser/Thr) to be fully available for affinity interaction with its analyte—a tumor-associated antibody. The step by step building process of glycan biosensor development was comprehensively characterized using a battery of techniques (scanning electron microscopy, atomic force microscopy, contact angle measurements, secondary ion mass spectrometry, surface plasmon resonance, Raman and energy-dispersive X-ray spectroscopy). Results suggest that electrochemical oxidation of graphene SPE preferentially oxidizes only the surface of graphene flakes within the graphene SPE. Optimization studies revealed the following optimal parameters: activation potential of +1.5 V vs. Ag/AgCl/3 M KCl, activation time of 60 s and concentration of HSA of 0.1 g L−1. Finally, the glycan biosensor was built up able to selectively and sensitively detect its analyte down to low aM concentration. The binding preference of the glycan biosensor was in an agreement with independent surface plasmon resonance analysis.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Biosensor, Electrochemistry, Glycan, Graphene screen-printed electrodes, Tn antigen
in
Sensors (Switzerland)
volume
19
issue
24
article number
5409
publisher
MDPI AG
external identifiers
  • scopus:85076287141
  • pmid:31818011
ISSN
1424-8220
DOI
10.3390/s19245409
language
English
LU publication?
yes
id
d0b575e5-ddbb-4bbb-9064-296a4fa11bcd
date added to LUP
2020-04-23 12:13:52
date last changed
2020-09-16 04:44:25
@article{d0b575e5-ddbb-4bbb-9064-296a4fa11bcd,
  abstract     = {<p>The study describes development of a glycan biosensor for detection of a tumor-associated antibody. The glycan biosensor is built on an electrochemically activated/oxidized graphene screen-printed electrode (GSPE). Oxygen functionalities were subsequently applied for covalent immobilization of human serum albumin (HSA) as a natural nanoscaffold for covalent immobilization of Thomsen-nouvelle (Tn) antigen (GalNAc-O-Ser/Thr) to be fully available for affinity interaction with its analyte—a tumor-associated antibody. The step by step building process of glycan biosensor development was comprehensively characterized using a battery of techniques (scanning electron microscopy, atomic force microscopy, contact angle measurements, secondary ion mass spectrometry, surface plasmon resonance, Raman and energy-dispersive X-ray spectroscopy). Results suggest that electrochemical oxidation of graphene SPE preferentially oxidizes only the surface of graphene flakes within the graphene SPE. Optimization studies revealed the following optimal parameters: activation potential of +1.5 V vs. Ag/AgCl/3 M KCl, activation time of 60 s and concentration of HSA of 0.1 g L<sup>−1</sup>. Finally, the glycan biosensor was built up able to selectively and sensitively detect its analyte down to low aM concentration. The binding preference of the glycan biosensor was in an agreement with independent surface plasmon resonance analysis.</p>},
  author       = {Kveton, Filip and Blsakova, Anna and Lorencova, Lenka and Jerigova, Monika and Velic, Dusan and Blixt, Ola and Jansson, Bo and Kasak, Peter and Tkac, Jan},
  issn         = {1424-8220},
  language     = {eng},
  number       = {24},
  publisher    = {MDPI AG},
  series       = {Sensors (Switzerland)},
  title        = {A graphene-based glycan biosensor for electrochemical label-free detection of a tumor-associated antibody},
  url          = {http://dx.doi.org/10.3390/s19245409},
  doi          = {10.3390/s19245409},
  volume       = {19},
  year         = {2019},
}