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Cross-Linker Mediated Biofunctionalization of Single Wall Carbon Nanotubes with Glucose Oxidase

Yadav, Poonam ; Ajore, Ram LU and Bharadwaj, Lalit M (2009) In AZO NANO
Abstract
Covalent attachment of biomolecules to the surface of carbon nanotubes provides an architecture for three dimensional arrays of sensor molecules (i.e. enzymes) for potential biosensor application. Present work reports a simple two-step reaction for immobilization of glucose oxidase on single walled carbon nanotubes (SWCNTs). This method is as efficient as conventional methods for biofunctionalization of SWCNT with enzyme. Moreover, it overcomes structural losses of SWCNTs and minimizes reaction steps involved in this process previously. Cross linkers 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide and N-hydrosuccinimide were employed for omitting the acylation step through formation of stable intermediates. The inference of efficacy of the... (More)
Covalent attachment of biomolecules to the surface of carbon nanotubes provides an architecture for three dimensional arrays of sensor molecules (i.e. enzymes) for potential biosensor application. Present work reports a simple two-step reaction for immobilization of glucose oxidase on single walled carbon nanotubes (SWCNTs). This method is as efficient as conventional methods for biofunctionalization of SWCNT with enzyme. Moreover, it overcomes structural losses of SWCNTs and minimizes reaction steps involved in this process previously. Cross linkers 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide and N-hydrosuccinimide were employed for omitting the acylation step through formation of stable intermediates. The inference of efficacy of the present methodology is based on the final outcome of the reaction, in terms of the number of glucose oxidase molecules immobilized on SWCNT. Biofunctionalization of SWCNTs was characterized by fourier transform infra red spectroscopy, ultraviolet-visible spectroscopy, elemental analysis and atomic force microscopy (Less)
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published
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AZO NANO
DOI
10.2240/azojono0130
language
English
LU publication?
no
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d10b62c2-5ae6-420f-9af9-0bb0a0c71eaa
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2017-07-25 12:18:33
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@article{d10b62c2-5ae6-420f-9af9-0bb0a0c71eaa,
  abstract     = {Covalent attachment of biomolecules to the surface of carbon nanotubes provides an architecture for three dimensional arrays of sensor molecules (i.e. enzymes) for potential biosensor application. Present work reports a simple two-step reaction for immobilization of glucose oxidase on single walled carbon nanotubes (SWCNTs). This method is as efficient as conventional methods for biofunctionalization of SWCNT with enzyme. Moreover, it overcomes structural losses of SWCNTs and minimizes reaction steps involved in this process previously. Cross linkers 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide and N-hydrosuccinimide were employed for omitting the acylation step through formation of stable intermediates. The inference of efficacy of the present methodology is based on the final outcome of the reaction, in terms of the number of glucose oxidase molecules immobilized on SWCNT. Biofunctionalization of SWCNTs was characterized by fourier transform infra red spectroscopy, ultraviolet-visible spectroscopy, elemental analysis and atomic force microscopy},
  author       = {Yadav, Poonam  and Ajore, Ram and Bharadwaj, Lalit M},
  language     = {eng},
  series       = {AZO NANO},
  title        = {Cross-Linker Mediated Biofunctionalization of Single Wall Carbon Nanotubes with Glucose Oxidase},
  url          = {http://dx.doi.org/10.2240/azojono0130},
  year         = {2009},
}